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Biomedical subjects

B Grassi

Publications and source records attributed to B Grassi.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

Cell-mediated immunity in male fertility. Immunological infertility in man.

The leukocyte migration inhibition test on agarose was performed on 210 patients with different andrological disorders: oligozoospermia following primarily testicular disorders, oligozoospermia following primarily non testicular disorders and idiopathic oligozoospermia. This test was most applicable in disorders involving testicular damage and in cases of varicocele with altered blood flow. It would appear that cell-mediated immunity is involved in the pathogenesis of the consequences of these disorders.

Adult↗
PubMedDate not supplied

Low-dose ARA-C and 1(OH) D3 administration in acute non lymphoid leukemia: pilot study.

BACKGROUND AND METHODS: Vitamin D3 metabolites have been shown to be able to induce monocytic differentiation both in vitro and in vivo. In this paper we report the preliminary results of an uncontrolled clinical trial where low doses of ARA-C and 1(OH)D3 were administered to patients affected by acute non lymphoid leukemia. The achievement of complete or partial remission was recorded. Morphological and cytochemical studies were performed in order to control the blastic populations under therapy. Immunocytochemical studies were also performed in some patients in order to detect the presence of 1,25(OH)2D3 receptors in the blast population. Seventeen percent reached complete remission and 45% reached only a partial remission. RESULTS AND CONCLUSIONS: The results are in line with those showing that low doses of ARA-C are an effective treatment in this type of leukemia. In some cases (7/11), a monocytic/monoblastic shift was detected. The demonstration of 1,25(OH)2D3 receptors in some blasts is also reported. Thus it is possible to suggest that the vitamin D metabolite displays "in vivo" the differentiating activity already shown "in vitro".

Adult↗
PubMedDate not supplied

Synergistic effects of alpha interferon and 1,25 dihydroxyvitamin D3: preliminary evidence suggesting that interferon induces expression of the vitamin receptor.

BACKGROUND: To active metabolite of vitamin D3-1,25(OH)2D3-is a well-known differentiation inducer. The addition of this metabolite to sensitive cell cultures inhibits proliferation and induces monocytic-macrophagic differentiation. Alpha interferon may also inhibit proliferation and increase the expression of some surface antigens in some neoplastic cells. In the present report, we describe the synergistic activity of these two drugs on U-937 and on cultured cells from a leukemic patient. METHODS: Proliferation was studied by 3H-thymidine incorporation; differentiation markers were evaluated immunologically by monoclonal antibodies and by cytochemical tests. Phagocytosis and NBT reduction test were also performed in order to confirm the differentiating properties of these drugs. Finally, the expression of the 1,25(OH)2D3 receptor was evaluated by immunochemical methods. RESULTS: After culturing these cells for 72 hours in the presence of 1,25(OH)2D3, cell proliferation was reduced and the expression of some phenotypic and functional markers suggested monocytic-macrophagic differentiation. Alpha interferon and 1,25(OH)2D3 synergistically inhibit the proliferation of U-937 cells. Alpha interferon increased the expression of the 1,25(OH)2D3 receptor in U-937 cells. CONCLUSIONS: The reported results confirm the synergistic activity of INF and 1,25(OH)2D3 on cell proliferation in monoblastic cells. The possible role of the increased expression of the vitamin receptor in cells cultured in the presence of INF is discussed.

Calcitriol↗
PubMedDate not supplied

A case of B-CLL expressing P-glycoprotein in peripheral leukocytes. Methodological considerations.

P-glycoprotein, the molecular marker of multidrug resistance, was investigated by means of the specific monoclonal antibody C219 in circulating lymphocytes obtained from a patient affected by B-CLL, who had undergone chemotherapy with chlorambucil and CVP. Dot-immunobinding and immunoblotting revealed the presence of P-glycoprotein in the cell lysate, but the immunoperoxidase method and indirect immunofluorescence carried out with a fluorescence microscope and a cytofluorimeter failed to detect the protein. Some methodologic problems are discussed.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMedDate not supplied

P-glycoprotein expression in multiple myeloma.

P-glycoprotein expression was investigated in ten drug resistant patients suffering from multiple myeloma. The immunoperoxidase method on bone marrow plasma cells and immunoblotting were performed by using the monoclonal antibody C219. Five patients resistant to protocols including vincristine, melphalan and doxorubicin were found to express P-glycoprotein. The immunoperoxidase method appeared to be sensitive and suitable for P-glycoprotein detection in bone marrow samples.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMedDate not supplied

P-glycoprotein and drug resistance in acute leukemias and in the blastic crisis of chronic myeloid leukemia.

The aim of this work is to evaluate the relationship between P-glycoprotein expression in circulating blasts and clinical response in patients suffering from acute lymphoblastic leukemia, acute non-lymphoblastic leukemia, and chronic myeloid leukemia in either lymphoid or myeloid blastic crisis. The results obtained show that: a) patients whose blasts express P-glycoprotein are resistant towards protocols including Doxorubicin, Daunorubicin, Etoposide, Mithramycin, Vincristine; b) P-glycoprotein can be expressed constitutively in some cases; c) P-glycoprotein does not appear to be the only mechanism responsible for resistance towards anthracyclines and Etoposide.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMedDate not supplied

Automatic differential blood counting in plasma cell leukemia.

The authors report a case of primary plasma cell leukemia and two cases of multiple myeloma with a large number of circulating plasma cells. The display of Technicon H-1 shows a very high "cloud" in the area of large unstained cells. A massive invasion of the basophil area was not found. Differences between these results and those found in different lymphoid leukemias are discussed.

Aged↗
PubMedDate not supplied

Rubidium is a possible supporting element for bone marrow leukocyte differentiation.

In the present paper the possible role of rubidium (Rb) on leukocyte differentiation is evaluated. When the metal was added to human mononuclear bone marrow cells cultured for 14 days in soft agar, it was able to increase the number of clusters and to reduce the number of colonies. This activity depended upon the presence of conditioned medium. Rb induced a faster differentiation of HL 60 cells cultured in a medium able to promote granulocyte differentiation. The metal did not show any activity on continuously cultured HL 60. These data suggest that Rb is a supporting element for cell differentiation. Toxicity tests on rats are also reported.

Animals↗