Biomedical subjects

B H Wu

Publications and source records attributed to B H Wu.

At least 19 recordsLinked to original sources

PubMed2004

QTL analysis of quality traits in an advanced backcross between Prunus persica cultivars and the wild relative species P. davidiana.

Genetic control of the different attributes involved in peach quality has been investigated in an advanced backcross population derived from a cross between Prunus davidiana clone P1908, a wild parent with poor agronomic performance, and a commercial variety, Summergrand. A total of 24 physical and biochemical traits were investigated. Quantitative trait loci (QTLs) were detected for all the traits studied. We identified alleles from P. davidiana with agronomically favorable effects regarding fruit and stone sizes, sugar and acid concentrations and red flesh coloration, in clear contrast to its phenotype. We identified three main regions of the genome where alleles from P. davidiana had negative effects on multiple traits. In other regions, co-locations of QTLs with opposite effects on quality traits were also detected. We discuss the nature of these co-locations in the light of the probable physiological mechanisms involved. Strategies to cope with negative correlations between favorable traits and co-locations of P. davidiana alleles with negative effects on quality traits and positive effects regarding resistance to powdery mildew are discussed from a breeding point of view.

Agriculture↗

PubMed2003

Modelling citrate metabolism in fruits: responses to growth and temperature.

Citrate production and degradation during the last stage of fruit development were modelled by representing the fluxes through the enzymes of the citrate cycle and the malic enzyme, the transport of metabolites between the cytosol and the mitochondria, and the stoichiometry equations that relate these reactions. After solving the corresponding system of equations, the rate of citrate synthesis (or degradation) was expressed as a simple function of temperature, mesocarp weight, and respiration. The model was applied to peach fruit, and its parameters were estimated from the data of a 2-year field experiment. The predictions of the model were in agreement with experimental data. Simulations were made to analyse the responses to variations of temperature and fruit growth. Increasing fruit growth before stone hardening stimulated citrate production, while increasing fruit growth after stone hardening reduced it. Delaying the date at which the maximum growth rate was reached enhanced citrate production during most of the period. In the last weeks before harvest, increasing temperature depressed citrate production, while, at the beginning of the period studied, it enhanced it.

Citrates↗

PubMed2002

A study on the sectional anatomy of the oculomotor nerve and its related blood vessels with plastination and MRI.

To obtain normal images and sectional anatomical data of the oculomotor nerve and its related arteries, the optimal angles and the length of intracisternal segment of the oculomotor nerve were measured on MPR images. Meanwhile, the relationships between the nerve and the basilar, posterior cerebral, superior cerebellar and posterior communicating arteries were observed from plastination slices, original images, MPR and MIP images. MRI revealed similar results to the plastination sections. The intracisternal segment of oculomotor nerve formed an angle with the posterior plane of the brainstem. The angle was significantly smaller in individuals under 10 and over 50 years old ( P<0.05), and there was no marked difference in the angle between the oculomotor nerve and the median sagittal plane among the different groups ( P>0.05). Shift of the basilar artery was more likely to be found in aged individuals. Most of the posterior cerebral and superior cerebellar arteries were close to the nerve, and a few of them seemed to compress it; for the posterior communicating artery, only the embryonic type was close to or seemed to compress the nerve. MRI is an accurate imaging technique for determination of the relationship of the oculomotor nerve to its related arteries.

Adolescent↗

PubMed1989

[The development of otorhinolaryngology in the northern Song dynasty].

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China↗

PubMed1989

[The polypeptides of mumps virus and their synthesis in infected chick embryo cells].

Strain ME of mumps grown in chick amniotic cavity and purified by differential centrifugation combined with sucrose gradient centrifugation. The structural polypeptides of purified virion were analyzed by SDS polyacrylamide gel electrophoresis. Eleven polypeptides were found. Their molecular weight were between 35K-72K dalton. In addition polymers of HN protein and F1 the large subunit of F protein were detected. Hela, Vero and CE cells were infected with ME strain of mumps virus. The CE cell reveals the most sensitive host cell. CE cell infected with mumps virus and labeled with [35S]-Met, SDS-PAGE and examined by autoradiography. It was found at least eight polypeptides were synthesis in host cells and their molecular weight were between 26.5K to 94K dalton. The time course of the synthesis of polypeptides in the cells were studies. Palse-Chase experiments demonstrated the posttranslational process of Fo----F.

Animals↗

PubMed1988