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B Hagmar

Publications and source records attributed to B Hagmar.

At least 55 records · Page 3Linked to original sources
PubMed1986

Metastasizing myxopapillary ependymoma of the sacrococcygeal region. A clinico-pathologic, light- and electronmicroscopic, immunohistochemical, tissue culture, and cytogenetic analysis of a case.

A case of recurrent and metastasizing subcutaneous myxopapillary ependymoma of the sacrococcygeal region in a 44-year-old man is reported. The tumor was characterized light microscopically by numerous papillary projections, lined by epithelium-like cells, with a variable degree of polymorphism. Histochemical analysis relating to glucosaminoglycans indicated the presence of hyaluronic acid and chondroitin-4- and/or 6-sulfate. Using immunoperoxidase techniques, glial fibrillary acidic protein (GFAP) and S-100 protein were demonstrated within the tumor cells. Ultrastructurally, the tumor cells were characterized by an abundance of intermediate cytoplasmic filaments, prominent interdigitating cytoplasmic projections, the formation of desmosomes and external lamina-like material. The growth pattern in the tissue culture of this tumor is described, and the ultrastructural appearance of the cultured cells revealed features similar to the primary and recurrent tumor. Chromosome analyses by the G-banding technique of early generations of cultured tumor cells revealed a normal diploid stemline without gross chromosomal deviations. Among the different variant cells and clones recorded, those with X chromosome deviations were of special interest since gonosomal deviations have previously been observed in other types of glioma. The differential diagnosis against adenopapillary carcinoma, chordoma and malignant teratoma is briefly discussed.

Adult↗
PubMed1986

Characterization of two murine tumor sublines selected purely in vivo for specific metastatic patterns.

We have attempted to clone an ascites tumor, MCB 21-AA, which was originally a fibrosarcoma, by transplantation of spontaneous metastases from the lungs or a lymph node to a subcutaneous site, and by repetition of this procedure ten times. We isolated two tumor sublines, LU (from lung) and LN (from lymph node), which differ from the original tumor in several cell characteristics. When tested for the formation of experimental metastasis, these sublines disseminate in the same manner as the original tumor. We interpret this finding as an argument against the widely held view that metastasis is a highly selective cloning process.

Animals↗
PubMed1985

Dissemination pattern of two immunologically induced transplanted malignant lymphomas in C57 B1/6J mice.

The need for syngenic tumors to elucidate factors in lymphoma dissemination prompted us to induce new lymphomas in a series of inbred C57B1/6J mice. The method of induction, immunosuppression and simultaneous immunostimulation, had a high mortality rate, but gave us 2 transplantable lymphomas. These tumors are described and compared in the present paper, with emphasis on their dissemination patterns. Both tumors disseminated rapidly from tail transplants to lymphoid organs, lungs and liver. They were characteristically dissimilar, however, in their s.c., i.p. and i.v. transplantabilities.

Animals↗
PubMed1985

Altered experimental metastasis pattern by proteolytic cell treatment.

We have studied some cellular characteristics and the transplantability of a newly induced squamous cell carcinoma, Sq1-SC, in comparison with the ascites-transformed subline of the same tumor, Sq1-AA. We could demonstrate that the AA tumor differed from the SC tumor in the pattern of intravenously induced 'experimental metastases'. The SC tumor preferentially gave rise to extrapulmonary tumor colonies ('metastases'), while the AA tumor exclusively gave rise to lung colonies. Comparison with the ascites tumor growing in solid form subcutaneously (AS tumor) shows that the enzymatic treatment, which is necessary to bring solid tumors into viable and dissociated suspensions, can have a decisive influence on tumor cell lodgement in vessels and metastasis.

Animals↗
PubMed1984

Effects of fine-needle aspiration and other biopsy procedures on tumor dissemination in mice.

Using five syngeneic tumor-host systems in mice, we studied the influence of fine-needle aspiration biopsy, incisional biopsy, and excisional biopsy on tumor death rates. Guided by results from pilot studies a very strict test protocol was developed which made possible a comparison between the tumor systems in spite of their differences in tumor growth rates and dissemination patterns. The compiled results from the experiments did not reveal any differences in tumor death rates, neither between the test groups and their controls, nor between the three biopsy methods: fine-needle aspiration biopsy, incisional biopsy, and excisional biopsy. Additional experiments confirmed, however, earlier observations that tumor cell seeding and tumor outgrowth by way of the aspiration needle track may occur under extreme test conditions.

Animals↗
PubMed1984

The tumor-cell-releasing machine.

We describe the construction of a machine for the injection of 10- to 100-microliter volumes of viable cells in suspension. The cells are maintained in a tissue culture environment and can be injected according to a preprogrammed schedule. Preliminary results show that the tumor incidence and metastasis pattern of TA3Ha cells injected intravenously into mice are the same if the cells are injected in a single dose, or administered in 20 fractions during 3 h.

Animals↗
PubMed1984

The elimination of i. v.-injected tumor cells. Studies with 125IUDR labelled cells in 4 syngeneic murine tumor systems.

We have studied the elimination of radiolabelled (125IUDR) i.v. injected tumor cells. We have used four different murine tumors with different abilities to metastasize and with different metastasis patterns. The elimination of the tumor cells was determined by gamma-countings from different organs. The results were compared with the elimination of heat killed and x-ray sterilized tumor cells. We found a poor correlation between on the one hand the clearance of tumor cells from the blood and distribution of radiolabelled cells and on the other hand the ability of the tumors to metastasize and the metastasis distribution upon i.v. injection. The radioactivity outside the first capillary bed seemed mainly to come from dead cells.

Animals↗
PubMed1984

Benign solitary neurilemoma (Schwannoma). A correlative cytological and histological study of 28 cases.

A correlative histological and cytological study of 28 cases of solitary neurilemoma is presented. The typical histological mixture of Antoni type A and B tissue correlated well with the cytological findings. In the smears, the Antoni type A tissue was represented by tissue fragments with a fibrillar ground substance and slender, spindle-shaped cells forming obvious Verocay bodies in 24 cases, and the Antoni type B tissue by loose, microcystic fragments with single, elongated nuclei sometimes looking like fish-hooks and with indistinct, slender, cytoplasmic processes. The ancient neurilemomas, a variant of the ordinary neurilemomas, in this series were characterised both histologically and cytologically by nuclear hyperchromasia and polymorphism, which it is important to be aware of in order to avoid an erroneous diagnosis of sarcoma or probable sarcoma. The differential diagnosis is discussed and it is concluded that diagnosis of neurilemoma by fine-needle aspiration biopsy is possible, particularly when Verocay bodies and/or a fibrillar ground substance are present in the smears. It is stressed that fine-needle aspiration biopsy is an important aid to pre-operative diagnosis of neurilemoma, and thus to planning of the operative treatment.

Adolescent↗
PubMed1984

Diagnosis and prognosis in malignant pleural mesothelioma: a prospective study.

In a prospective study of 336 consecutive patients with long-term pleural effusions, 32 cases of malignant mesothelioma were found. Microscopic examination of pleural tissue specimens, preferably selected at thoracoscopy, proved superior to pleural fluid analysis as an aid to correct diagnosis. The epithelial proved to be the most common type of malignant mesothelioma. As an example of the mesothelial cells' multipotent ability, malignant cells were seen transforming into fat-like cells with lipid-containing vacuoles. In the fibrous tumour type, cytological examination of pleural fluid revealed only normal cells. Cells with malignant features were seen in fluid samples from epithelial and biphasic tumour types. The malignant cells often formed tubuli-like aggregates which could be mistaken for adenocarcinoma. Hyaluronic acid was more frequently detected in tissue specimens than in the pleural fluid samples. The morphological type and the patient's age had an impact on the survival time, whereas sex and extensive surgical treatment seemed less important.

Aged↗
PubMed1983

Local tumour cell seeding by fine-needle aspiration biopsy. A semiquantitative study.

We have performed fine-needle biopsies from solid or ascites-growing mouse tumours. The biopsies were taken via striated muscle or the peritoneal cavity of healthy mice syngeneic with the tumours and the tumour-bearing animals. The incidence of tumour takes in these new mice were compared with the intramuscular or intraperitoneal transplantability of the tumours. In order to estimate more accurately the number of seeded cells, we also performed aspiration biopsy through the leg muscles of healthy animals into ascites-growing tumours labelled with 125IUDR. The resulting radioactivity in the leg muscle was compared with the radioactivity of the ascites tumour cells. The studies indicate that one may seed 10(3)-10(4) cells, and sometimes more, into the needle track with fine-needle aspiration biopsy from extremely cellular and dedifferentiated tumors.

Animals↗
PubMed1982

125IUDR labelling efficiency and cytotoxicity for murine tumours in vivo and in vitro.

We have reviewed the literature on 125IUDR (5-iodo-2-deoxyuridine), the DNA label of choice for cell distribution studies. In previous studies, the cytotoxicity in relation to labeling efficiency has often been inadequately investigated or reported. We have studied four syngeneic mouse tumours and compared in vitro with in vivo labelling procedures. Ascites tumours could be effectively labelled in vivo by IP injections of 60-80 muCi per mouse during 24 h, without apparent cytotoxicity. Comparable in vitro labelling was also effective, but caused a dose-related cytotoxicity, as measured by growth rate and transplantability. Comparison with 'cold' IUDR disclosed that the toxicity was not chemical, but radiological. Our attempt to label a solid lymphoma in vivo was unsuccessful, obviously because the tumour grows too slowly. We conclude that 125IUDR-labelling is suitable for cell distribution studies, but in view of labelling efficiency versus cytotoxicity the procedure has to be adjusted to each new tumour individually.

Animals↗
PubMed1982

Alveolar rhabdomyosarcoma: a cytogenetic and correlated cytological and histological study.

A correlative, histologic and cytologic study of seven cases of alveolar rhabdomyosarcoma and chromosome analysis using the G-banding technique (in case 1) is presented. The histologic pattern was characterized by pseudo-alveolar structures, separated by fibrous septa. Primitive, dark, round cells predominated, but differentiated rhabdomyoblasts were found in all cases, and so were multi-nucleated giant cells. Unequivocal cross striation was seen in two cases. Cytologically, the tumors were highly cellular and small, undifferentiated tumor cells predominated. Large polymorphous, sometimes multi-nucleated, cells often with an elongated cytoplasm and eccentric nucleus were observed in all cases. The cell-types found could be well correlated to those identified in the histologic sections. The cytogenetic study revealed a karyotype profile, showing three 2q markers and only one chromosome type No. 13. The observations are different from the karyotype profile of embryonal rhabdomyosarcoma. However, further studies are necessary to elucidate this question.

Adolescent↗
PubMed1981

Malignant giant cell tumor of soft tissues: a clinicopathologic, cytologic, ultrastructural, angiographic, and microangiographic study.

A clinical, angiographic, pathologic-anatomic, and cytologic study of 12 cases of malignant giant cell tumor of soft tissues is presented. Preoperative angiography was performed in five cases, one of which was also studied by microangiography. Aspiration cytologic examination was performed in five cases and electron microscopy in one case. In nine cases the tumors were deeply situated and in three they were subcutaneous. In six cases the tumors were located within the lower extremity, in four cases in the upper extremity and shoulder, in one case retroperitoneally and in one case in the neck. Except for one case, the follow-up period was 1--14 years. Seven of the patients had died from metastasizing tumor disease at the time of follow-up. High vascularity and rapid circulation characterized the tumors at preoperative angiography. Conventional histology, aspiration cytology, and electron microscopy disclosed histiocyte-like cells, fibroblast-like cells, and multinucleated giant cells of both osteoclast-like benign and pleomorphic malignant type, in varying proportions. The histiocyte-like cells and the giant cells exhibited phagocytic activity at these examinations. The finding of phagocytosing pleomorphic malignant cells and giant cells of osteoclast type in aspiration cytologic material strongly favors the diagnosis of malignant giant cell tumor of soft tissues.

Adult↗
PubMed1981

Alteration of a tumor cell distribution pattern by cytochalasin B.

Cytochalasin B pretreatment of tumor cells alters the distribution pattern of metastases after i.v. injection. We now have studied Cytochalasin B cell pretreatment affects the i.v. distribution and elimination of 125IUDR-labeled TA3-Ha tumor cells. We found no differences between control and CB-treated cells in their initial distribution. but 4-8 hours after the injection, in the redistribution phase, there were more CB cells than control cells extrapulmonally. Rather than a consequence of CB paralysis, we interpret the results in terms of altered surface properties of CB cells during their recovery.

Animals↗
PubMed1981

Leiomyosarcoma of the soft tissue. A correlative cytological and histological study of 11 cases.

Leiomyosarcoma of the soft tissues is a well-defined and characteristic entity histologically but correlative cytomorphological studies are lacking. A correlative histological and cytological study of 11 cases is presented. The leiomyosarcomas were characterized histologically by elongated tumour cells arranged in bundles intersecting each other at wide angles. The nuclei were elongated and often blunt-ended, tending to be aligned in tandem-position or in rows. The smears from leiomyosarcoma were poor in tumour cells and most cells were arranged in clusters of strands. The characteristic blunt-ended cells and nuclei of leiomyosarcoma could be identified in the smears and the nuclei were focally arranged in rows and sometimes in parallel. Nuclear atypia permits diagnosis of malignancy, although the atypia was generally not very pronounced. We consider that a knowledge of the corresponding histological features is mandatory for differentiating cytologically between various soft tissue sarcomas, including leiomyosarcomas. Further correlative cytological and histological studies of other soft tissue sarcomas are necessary for a more valid interpretation of the smears from leiomyosarcomas.

Adult↗