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Biomedical subjects

B Heinz

Publications and source records attributed to B Heinz.

At least 19 recordsLinked to original sources
PubMed1999

A dynamic and direct visualization model for the study of nasal airflow.

OBJECTIVE: To evaluate nasal airflow characteristics during physiologic breathing in normal and pathologic conditions. DESIGN: The choana of an anatomical human model was connected to a pump that simulated physiological pressure changes in the upper airway system. Normal ambient air was used as medium. The airstream was marked with aerosolized water particles, and was observed through an exact but translucent replica of the original nasal septum. RESULTS: In physiologic conditions the airflow is mixed. Turbulence is clearly visible even with low flow velocities. There is less turbulence with lower flow rates. The nasal airflow follows a triphasic pattern of acceleration, near-steady state, and deceleration. Turbulence is prominent in the first and third phases. The main flow stream passes through the middle meatus at all rates. Hypertrophic mucosal membranes and turbinates increase the proportion of air passing the middle meatus. With decongested turbinates, flow distribution is more even. After turbinectomy there is a significant amount of airflow passing along the floor of the nose. The olfactory region is aerated only toward the end of inspiration and during the entire expiration phase. CONCLUSIONS: This model allows the investigation of airflow distribution and turbulence under physiologic conditions and the examination of the influence of pathologic conditions on these parameters. Overzealous trimming of turbinates results in an unphysiologic distribution of airflow.

Airway Resistance↗
PubMed1998

[Epistaxis strategy--experiences with the last 360 hospitalizations].

BACKGROUND: The purpose of this study was to analyze the therapy concept used in the management of severe epistaxis in order to optimize the quality of our treatment and to arrive at an optimal therapeutic concept. MATERIAL AND METHODS: The study was performed as a retrospective analysis of the charts of all 335 patients treated as inpatients for epistaxis over a period of 6 years. RESULTS: An increased rate of complications was found in the following three situations: 1. Age > 65 years, 2. chronic obstructive pulmonary disease, 3. several systemic illnesses. Recurrence was increased with septal perforations and Osler's disease. CONCLUSIONS: Localization of the site of bleeding is the first priority and allows treatment of bleeding with bipolar electrocautery. If the bleeding vessel is not found, insertion of a Merocel pack is recommended. Patients with persistent or recurrent bleeding are treated in the operating theater under general anesthesia if they are at an elevated risk for complications or have a septal deviation which is clinically significant. The nose is systematically visualized with the endoscope, bleeding vessels are coagulated under endoscopic control, and, where appropriate, a septoplasty is performed. In the remaining cases an epipharynx balloon is inserted, combined with an anterior pack. Angiography with superselective embolization, external ligation of the ethmoidal arteries, and endonasal obliteration of the sphenopalatine artery is reserved for cases where the measures discussed above have failed.

Adult↗
PubMed1998

Treatment of gingival recession with titanium reinforced barrier membranes versus connective tissue grafts.

It was the aim of this study to compare barrier membrane therapy with connective tissue grafts in the treatment of recession defects. Fifteen patients with 2 paired vestibular recession defects participated in the study. After thorough scaling and root surface conditioning with tetracycline-HCl, defects were randomly assigned to 1 of 2 treatment modalities. In one of the defects, a trapezoidal mucoperiosteal flap was prepared, and a titanium reinforced expanded polytetrafluoroethylene membrane was adapted to the defect and secured by a sling suture. The membrane was subsequently covered by a coronally positioned flap secured with crown attached sutures. The other defect was treated with a connective tissue graft employing the "envelope technique." Twelve months after therapy, both treatment modalities showed significant root coverage and probing attachment gain. Gingival recession decreased from 3.6+/-0.7 mm to 0.5+/-0.5 mm for membrane treated defects and from 3.6+/-1.0 mm to 0.5+/-0.5 mm following use of grafts, corresponding to mean root coverages of 87.1% and 86.9%, respectively. Mean gains of keratinized tissue of 1.5 mm (membrane) and 2.5 mm (graft) could be observed. Thus, the results of this study demonstrate that both titanium reinforced barrier membranes and connective tissue grafts can be successfully used to treat periodontal recession defects.

Adult↗
PubMed1997

Constant detection of CD2, CD3, CD4, and CD5 in fixed and paraffin-embedded tissue using the peroxidase-mediated deposition of biotin-tyramide.

Immunohistochemical methods are widely used for diagnostic purposes in histopathology. However, the use of most monoclonal anti-leukocyte antibodies is limited to frozen tissues. Initially, it was believed that formalin fixation in particular, which is the gold standard for morphological tissue preservation, destroys most of the antigen binding sites. In recent years, protease digestion and the introduction of microwave techniques have significantly enhanced the sensitivity of immunohistochemical techniques, and a variety of hidden antigen sites in formalin-fixed tissue have been retrieved for initially unreactive antibodies. It therefore became clear that many of the leukocyte antigens are not irreversibly destroyed but are most probably masked during the fixation process. We developed a technique combining optimized pretreatment of formalin-fixed tissue with a dramatic enhancement of the immunohistochemical sensitivity and named it the ImmunoMax method. The ImmunoMax method proves that by optimizing the technique at the following three levels it is possible to detect formalin-sensitive leukocyte antigens: (a) standard fixation of the tissue; (b) sufficient antigen unmasking; and (c) increasing the substrate turnover by multiplication of binding sites with subsequent enhancement of the immunohistochemical reaction. Using this optimized ImmunoMax method, we were able to detect CD2, CD3, CD4, and CD5 with conventional monoclonal antibodies in formalin-fixed, paraffin-embedded tissue specimens of various lymphoid tissues.

Antigens, CD↗
PubMed1996

Fabrication and strategic significance of a special resin composite splint in advanced periodontitis.

The usefulness of splinting of severely loosened teeth in advanced periodontitis has been discussed extensively, both positively and negatively. Retention of patients own teeth through the aid of this simple and inexpensive resin composite splint provides a satisfactory solution for many affected patients. The fabrication and placement techniques for splints for the anterior and posterior regions are described, as are additional indications for such splints, which are fabricated from resin composite and glass fiber bundles. Such splinting can be provided even for severely loosened, periodontally diseased teeth without danger of intraoperative tooth loss. Long-term retention of such teeth can be ensured only through a risk-oriented postoperative program of oral hygiene and regular examination of the splint.

Adolescent↗
PubMed1994

Nuclear accumulation of p53 in response to treatment with DNA-damaging agents.

A number of agents which damage DNA also trigger the nuclear accumulation of the tumor suppressor protein p53. Here we show the correlation with different p53 detection methods. As an example we investigated the effects of the cancer therapy drug mitomycin C on different mammalian cell lines. Our findings demonstrate that either the immunofluorescence techniques (indirect immunofluorescence staining or flow cytometric analysis) or ELISA or immunoblot assays are useful methods in detecting p53 accumulation. Simultaneously we measured DNA damage with the terminal deoxynucleotidyl transferase assay. Compatible data were obtained. Thus p53 accumulation may be used as indicator of DNA injury.

3T3 Cells↗
PubMed1991

[Use of autologous ear cartilage in eyelid surgery].

Autologous ear cartilage is relatively easy to procure in suitable form and sufficient quantity. At the donor site, there are, as a rule, no complications to be expected. Autologous ear cartilage without conjunctival coverage is of good use for replacing and lengthening the inner layer of an eyelid, not only to reconstruct large eyelid-defects, but also to give additional support and length. The cartilage graft will grow on well, provided the wound is well vascularized. This must not be expected with scarring or poor blood perfusion. The brittleness of the cartilage demands an adapted technique. Precautions to protect the cornea and to prevent adhesions in the fornix are to be taken.

Adolescent↗