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Biomedical subjects

B Helpap

Publications and source records attributed to B Helpap.

At least 181 records · Page 10Linked to original sources
PubMed1977

[Primary intratympanic meningioma (author's transl)].

A primary intratympanic meningioma is reported, which was histologically verified. 1 1/2 and 2 1/2 years after surgical removal, there were no signs of recurrence, neither clinically nor histologically. Sites of origin of the primary intratympanic meningiomas are discussed. Symptoms, diagnostics, differential diagnosis, therapy and prognosis are summarized in relation to nine cases of the literature.

Ear Neoplasms↗
PubMed1977

Malignant papillomatosis of the intrahepatic bile ducts.

A case of intrahepatic bile duct papillomatosis is reported which recurred several times and finally progressed into a mucin-secreting papillary adenocarcinoma. The paillomatosis predominantly involved the ductal system of the left hypoplastic hepatic lobe. The treatment consisted of a left partial hepatectomy. Hereby, a four-year survival was reached. While the prognosis of solitary papillomas in the distal choledochus and in the gallbladder is considered as good, the prognosis of the intra- and extrahepatic bile duct papillomatosis appears unfavorable. The experience in this case, as well as in the literature, shows that these papillomatoses have to be regarded as low-grade carcinomas.

Adult↗
PubMed1977

Cell-kinetical analyses of squamous cell carcinomas of the oral region and the effect of a combined therapy of 5 fluorouracil and irradiation. A contribution to the discussion about tumorcell-synchronization.

Out of 180 incubations of human biopsies 33 were from patients with malignant tumors of the oral region. In 9 cases it was possible to analyse the cell cycle of the tumor cells with the double labeling in vitro method (3H and 14C-thymidine). Histologically the tumors were keratinizing squamous cell carcinomas with high labeling indices. The generation time of the tumor cells ranged from 20 to 130 hours. The length of the DNA synthesis phase varied between 7.3 and 17.9 hours. With the knowledge of the cell-kinetic data the patients received an infusion of 5 Fluorouracil throughout the length or parts of the calculated G 1 phase. At the end of the 5 FU infusion there was a constant two-hour pause and after the duration of the calculated DNA synthesis phase the tumors were irradiated with 150 rd per session. The treatment was repeated until a total dose of 6000 rd was achieved. Under the combined therapy of 5 FU and irradiation the inoperable keratinizing highly differentiated squamous cell carcinomas regressed extraordinarily fast. The patients were free of pain after a very short time. No vital tumor tissue was visible in control biopsies. Some patients remained without any tumor recurrence now more than 2 years after onset of therapy. In the discussion it is stressed that the reason for the successful treatment of highly differentiated squamous cell carcinomas with 5 Fluorouracil and subsequent irradiation is not easily explained, because recent experimental findings and impulsecytophotometrical studies on human biopsy material suggest that both the synergistic action of thecytostatic drug and the irradiation as well as an altered recruitment of the tumor cells are responsible for the clinical success rather than a synchronisation effect.

Adult↗
PubMed1976

Wound healing of the brain of rats after cryonecrosis. Autoradiographic investigations with 3H-thymidine.

Histologic and autoradiographic studies were performed to investigate the cellular reactions during wound healing of the brain of rats after cryonecrosis. A parietal lobe was frozen for 30 s with a cryoprobe at a temperature of -196 degrees C. The survival time ranged between 12 h and 21 days. One h before killing, tritiated thymidine was injected intraperitoneally. Stripping film autoradiograms of the brain sections were made in the usual manner. A typical cryonecrosis develops 12 h after local freezing surrounded by an edematous tissue layer with activated mesenchymal and neurologlial cells. After 10 days a pseudocyst develops surrounded by highly cellular glial tissue. The pseudocyst is still recognizable 3 weeks later but without any remarkable cellular reaction. Autoradiographically the labeling indices of the fibroblasts, leptomeningeal,and endothelial cells in the periphery of the necrosis and the labeling indices of neuroglial, perivascular connective tissue, and microglial cells in the perinecrotic zone increase after 12 h and have their maximum between the 2nd and 3rd postoperative day. In the nonfrozen contralateral cerebral hemispheres, the labeling indices of neuroglial and mesenchymal cells show small peaks in the first 3 postoperative days,also. This can be explained by accompanying edema. There are no remarkable differences between frozen and nonfrozen parts of the brain 3 weeks after local freezing. The results underline the rapid repair of cryonecrosis.

Animals↗
PubMed1976

[Intracytoplasmic inclusions within cells of the breast and their diagnostic significance (author's transl)].

There were 700 tissue biopsies of the breast with and without carcinomas examined simultaneously by intraoperative histology and imprint cytology. In 20.2% of the cases with carcinoma the tumor cells showed peculiar intracytoplasmic inclusions, whereas in only 0.43% of the biopsies of the mamma without carcinoma such inclusions were to be found. Their morphologic variation and the histochemical pattern are discussed. It is pointed out that these intracytoplasmic inclusions may be a helpful histopathologic feature in the diagnosis of breast cancer.

Breast↗
PubMed1976

[Pulmonary rhabdomyosarcoma (author's transl)].

A 55-years old patient is reported who became ill with recurrent attacks of coughing and haemoptysis some 3 weeks before death. An extensive mediastinal tumor was demonstrated radiologically. Pathoanatomical examination showed a pleomorphic rhabdomyosarcoma of the lower left pulmonary lobe with metastases in the right lung and regional lymph nodes.

Autopsy↗
PubMed1976

Tissue culture, electron microscopic and enzyme histochemical investigations of extraadrenal paragangliomas.

Light and electromicroscopical as well as histochemical investigations were performed on three cases of extraadrenal paragangliomas. They were localized in the carotid body, tympanicum and cauda equina region. Tissue of two cases was cultivated in vitro in nutrient medium TCM 199. The tumours were classified as paragangliomas of the paraganglionic type with typical cell clusters, of the adenomatous and angiomatous type. The enzyme histochemistry showed a very high dehydrogenase activity. Ultrastructurally numerous typical osmiophilic granules could be observed in the cytoplasm of the tumour cells. In tissue culture only a minimal cellular proliferative activity could be detected. The few proliferating cell colonies showed mostly characteristics of epithelial tissue and sometimes a similar behaviour to cells of a ganglioneuroblastoma. The minimal proliferative activity in vitro is in good agreement with the proliferative behaviour of the extraadrenal paragangliomas in vivo.

Carboxylic Ester Hydrolases↗
PubMed1976

[Report on 2 cases of malignant hyperthermia with different clinical courses].

Two cases of malignant hyperthermia with different clinical courses are reported. The patients showed the classical signs of malignant hyperthermia consisting of tachycardia, tachypnoea, ocasional peripheral cyanosis, high body temperature as well as characteristic rise in serum enzymes. In one of the patients the symptoms were recognized early during the operation. The immediate commencement of therapy with ice-cooled. Ringer-Lactate-Solution, Procainmedication, Corticoids as well as physical body cooling favourably influenced the clinical course and the patient survived. In both cases the patients underwent succinylcholine and halothane anaesthesia, but the symptoms of the second patient appeared after the reduction of anaesthesia. In spite of vigorous therapy the hyperpyrexia resulted in heart arrest and death. Morphologically, both patients showed signs of preexistent myopathy with volumetric alterations of the muscle fibres, centralisation of the nuclei and acute muscle fibre necrosis. On the basis of the observed variable course, the various symptom complexes reported in the literature to data are reviewed. A detailed discussion of the "carrier problem" and the available treatment possibilities is also made. Realising that malignant hyperthermia is an inheritable disease, prophylactic measures such as, f.i. the issue of medical certificates to the patient and his relatives are suggested.

Adult↗
PubMed1975

[Comparative studies of simultaneous intraoperative frozen sections and imprint cytology of the mamma (author's transl)].

250 tissue biopsies of the mamma were examined simultaneously by intraoperative histology (frozen sections) and imprint cytology. In 95% of the cases there was a good agreement between the histologic and cytologic diagnoses. The reliability of the imprint cytology was tested in some complicated cases such as proliferating fibroadenomas, sclerosing adenosis and carcinomas and the significance of the imprint cytology for the diagnosis of difficult cases was pointed out in detail. The results have demonstrated, that such a cytologic examination can not be a substitute for intraoperative frozen sections - but the simultaneous cytologic examination of unfixed mamma biopsies can be a good searching method and can be helpful in the intraoperative diagnostic.

Adenocarcinoma, Mucinous↗
PubMed1975

Histological and autoradiographical findings in the immunologically stimulated spleen.

In order to estimate the role of a possible immunological coeffect on the splenic cellular proliferation during wound healing after mechanical or thermal lesions of internal organs, light microscopical and autoradiographical investigations with tritiated thymidine were performed on spleens of non-germfree rats, immunized by a single injection of sheep erythrocytes. Two days after an initial dissociation of preexisting germinal centers in the spleen a steep rise of the percentages of labeled cells in newly formed germinal centers occurs with a maximum on the fifth day. In the marginal zone an increased cell proliferation (mostly large lymphoid blasts) starts at 12 hours. In the lymphatic mantle zone a marked increase of labeled basophilic blasts (immunoblasts) can be observed with a maximum on the second day. The values are higher in the perifollicular B cell region than in the periarteriolar T cell region. In the red pulp of the spleen the highest percentages of labeled cells occur five days after the antigen injection. These findings in the spleen after a strong antigenic stimulation are characteristic for an immunological reaction of anamnestic type. In comparison with investigations of the spleen after cryolesions in internal organs such studies may be particularly helpful in judging the proportion of an unknown immunological reaction in the spleen after such an operation, which by tissue necroses and cellular destruction may have caused denaturation of self components.

Animals↗
PubMed1975

The cell proliferation of epithelial metaplasia in the prostate gland. An autoradiographic in vitro study.

In 147 autoradiographically examined prostate biopsy cylinders 5.4% squamous metaplasia and 6.0% transitional metaplasia were diagnosed. The average labeling index of squamous metaplasia was 4.3% and corresponded with values from non-keratinizing squamous epithelium in other locations. The labeling index of the transitional cell metaplasia was, in contrary, almost 10 times lower with an average value of 0.29% and corresponded with the values well known for the urothelium of the urinary bladder. Higher labeling indices were observed in 2 cases but these had chronic prostatitis. The autoradiographic results are in good agreement with the ultrastructural findings in which the metaplastic squamous epithelium corresponds with normal squamous epithelium by which the cellular proliferation takes place only in the stratum basale. On the other hand, transitional metaplasia exhibits DNA-synthesizing cells in the superficial cell layers just like in the urothelium.

Autoradiography↗
PubMed1975

[Histological, histochemical, and ultrastructural findings in malignant hyperthermia (author's transl)].

Malignant hyperthermia is a rare but severe complication of modern anesthesia, induced by halothane and succinylcholine. The syndrome is characterized by a rapid sustained and extreme rise in body temperature associated with muscular rigidity, tachycardia, tachypnoea and cyanosis. The lethality is about 60%. The present paper describes the histological, histochemical and electron microscopical findings performed on muscle biopsies of 3 patients with malignant hyperthermia (1 patient died) and a so called risk patient. In all patients morphological findings consistent with a pre-existent myopathy were found. Histologically there were acute necrotic muscular fibers as well as in types I and II, variations in the fiber diameter and centralization of the nuclei. In two cases even fibers that had a normal aspect in HE slides, showed a pathologic pattern after phosphorylase reaction. In addition to acute rhabdomyolysis, electron-microscopic investigations revealed cystic expansion of the cisterns of the sarcoplasmic reticulum with a peculiar proliferation of the sarcolemma. In a degenerating mitochondrium, a crystalline inclusion was identified. These findings support the pathogenetic concept of Britt and coworkers of a functional defect in the calcium release or binding mechanism of sarcoplasmic reticulum. Since it is known that malignant hyperthermia has a familial predilection, it seems very important that clinical, biochemical, and morphological investigations be performed such as CPK estimations and muscular biopsies not only of the patients but also of the relatives in order to rule out this type of latent myopathy.

Adult↗