PubMed Health⌕ Search

Biomedical subjects

B Herrmann

Publications and source records attributed to B Herrmann.

At least 127 records · Page 7Linked to original sources
PubMed1980

Selective guanosine phosphate deficiency in hepatoma cells induced by inhibitors of IMP dehydrogenase.

Inhibition of IMP dehydrogenase in AS-30D hepatoma cells in suspension culture resulted in a pronounced and selective reduction of guanine nucleotide pools. Total acid-soluble guanine nucleotides decreased to 40% and the content of GTP and GDP dropped to about 20% of control within 4 h when mycophenolate or ribavirin were used as the inhibitors. Induction of GTP deficiency was associated with a 50% rise in UTP and other uracil nucleotides. Guanosine rapidly reversed both the reduction of guanine nucleotide pools and the elevation of cellular UTP contents. Enzymatic nucleotide analyses in cell and tissue extracts after treatment with ribavirin indicated that ribavirin 5'-triphosphate was an effective substrate for yeast hexokinase, yeast phosphoglycerate kinase, and nucleosidediphosphate kinase from yeast or bovine liver. These results were confirmed in detail by the use of synthetic ribavirin 5'-triphosphate and 5'-diphosphate. The latter nucleotide analog was also a substrate of pyruvate kinase from muscle. Mycophenolate-induced GTP deficiency was associated with an arrest of hepatoma cell growth in suspension culture. Ribavirin, at an equimolar concentration, was much less effective in this respect. None of the two inhibitors had a detectable effect, however, in vivo when guanine or uracil nucleotides were assayed in liver. This indicated that an inhibition of de novo guanylate synthesis in vivo can be compensated by salvage pathway synthesis.

Animals↗
PubMed1978

[The use of childbirth alterations of the pubic bone for identification (author's transl)].

This paper discusses whether parturition or the number of parturition events can be determined from certain gross alterations, which are caused by pregnancy and parturition, found around the symphysis area of the pubic bone. The study is based on the analysis of 49 pairs of pubic bones, for which the number of parturitions and/or abortions were known. No criteria were found to be useful for a clear forensic diagnosis. However, this investigation did show a certain probability of correlation between the extension of grooves on the posterior pubic cortex and the number of parturitions. Examples of remarkable misleading conclusions in special cases are given.

Adult↗
PubMed1978

[Production of influenza virus recombinations].

Described in this paper is the preparation of influenza virus recombination partners, H3N2 and H3N1, by poly-infection of primary embryonic fowl cells with influenza virus A/Greifswald/6/74 (H3N2) or with A/Greifswald/1/76 (H3N2) and A/PR/8/34 (H0N1). Purification was based on four consecutive plaque isolations. The productivity of all recombination partners isolated in the embryonated fowl egg was higher than that of parent strain, H3N2. A recombination partner of H0N2 was obtained from poly-infection of isolated chorio-allantois membranes (CAM) with inactivated influenza virus A/PR/8/34 (H0N1) and A/Greifswald/6/74 (H3N2), the whole process being exposed to the action of serum anti-H3N1. Purification was undertaken via plaque passages, as well. The recombination partners could be characterised by determination of haemagglutination titres in the embryonated fowl egg and by reference to plaque diameters.

Allantois↗
PubMed1978

[Differentiation of matrix proteins of influenza A viruses using enzyme immunoassay].

Matrix protein is known as a type-specific structural protein of influenza viruses. An attempt has been made to find out whether or not strain-specific components could be detected from matrix protein, in addition to its type-specific antigen determinants. The technique of enzyme immune assay was chosen as the optional method to differentiate between matrix proteins of various influenza-A viruses. Antigen titration was undertaken of several matrix proteins, using two specific anti-matrix-protein sera in each case. Information regarding serological relationships between the tested matrix proteins of various influenza-A viruses was obtained from a quotient between the titres of one antigen, on the one hand, and the two anti-matrix-protein sera used in titration, on the other. Two matrix protein sub-types were established in the context of the influenza-A viruses tested. Sub-type M1 was attributable to older strains (A/PR/8 and A/FM/1), whereas the matrix protein of sub-type M2 was found to be present in more recent strains (A/Hongkong and A/Port Chalmers).

Antigens, Viral↗
PubMed1977

[Dependence of shrinkage on the mineral content of experimentally burned bones].

Quantitative changes of the human femur compacta have been studied by dilatometer investigation. Furthermore it was found that increasing mineral content of cortical bone is significantly correlated with a decreasing value of thermally induced shrinkage. Therefore, cortical thickness should be a useful criterion for sexing cremated remains.

Bone and Bones↗
PubMed1976

[Some new aspects in the study of human cremations (author's transl)].

Thermally induced reactions of bone mineral are outlined. The shrinkage of cremated bones is due to sintering of bone mineral. In an electron microscopic study changes of bone structure caused by thermal treatment and sintered mineral units are described. The problems of estimating the shrinkage factor and the use of cremation weight for identification and discussed. There is a significant difference between mean weights of cremations of senile males and females. The absolute difference indicates, that cremation weight is not a useful criterion for identification. Even in cremated bones, cuts and saw marks can be detected.

Age Factors↗
PubMed1976

Haemodialysis in imipramine poisoning? An experimental study.

In the last several years an increasing number of severe imipramine intoxications have been observed. Though standard principles for the treatment of acute tricyclic poisoning have been established, nonetheless there still exists doubt on the most effective method of tricyclic removal in cases of massive overdose. Haemodialysis was successfully employed until now but has not found general acceptance as only insignificant amounts of imipramine could be recovered from the dialysate. An experimental clearance study was undertaken using radiolabelled imipramine (14C-I) to obtain insight into the usefullness of haemodialysis in imipramine poisoning. 14C-I clearances which were calculated in a closed circuit dialysis system ranged between 18 ml/min and 48 ml/min depending on the constitution of the dialysate, i.e. aqueous or lipid solution. Surprisingly a rapid and significant uptake of imipramine by the plastic material (polyvinylchloride) of the extracorporeal blood line system was detected. This escape of imipramine from the blood into the tubings explains the poor recovery of tricyclics from the dialysate, which discredited haemodialysis as a therapeutical method in imipramine poisoning. The results of our experiments may offer a new method of rapid tricyclic elimination in severe imipramine intoxications.

Absorption↗
PubMed1976

[Radiological investigations of over-modelled skulls from New Ireland (Melanesia) (author's transl)].

The authors examined a series of overmodelled and decorated skulls from New Ireland which had been used as cult objects for ritual dances. The results are described briefly with a number of examples. Radiological examination provided no evidence that these had been chosen specifically according to age or sex. Of particular significance for ethnology is the finding of two skulls in which the jaws have been replaced before modelling by adapted pigs' mandibles. The non-destructive radiological investigation of artistic and cultural objects has a particularly long tradition in Berlin (eg. Baessler). With these examples we should like to stimulate further similar work.

Adult↗