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Biomedical subjects

B Hines

Publications and source records attributed to B Hines.

14 recordsLinked to original sources
PubMed2001

Genetic differentiation between mallee and tree forms in the Eucalyptus loxophleba complex.

The Eucalyptus loxophleba complex comprises two tree taxa (E. loxophleba ssp. loxophleba and ssp. supralaevis), two mallee taxa (E. loxophleba ssp. lissophloia and ssp. gratiae), and three rare mallee species, of which one, E. blaxellii, is included in this study. The genetic diversity and phylogenetic relationships between the taxa, particularly in relation to habit, were assessed using anonymous nuclear RFLP loci. The level of diversity in the taxa was high and similar to that detected in other eucalypt species. The populations showed low differentiation at both the subspecies and the species levels. Phylogenetic relationships showed some genetic separation between the tree and mallee habit but no separation of the two taxa within the tree habit or within the mallee habit. The genetic analysis does not support the recognition of E. gratiae as a separate species. The geographically restricted E. blaxellii showed similar levels of diversity to populations of the other widespread taxa of E. loxophleba.

Eucalyptus↗
PubMed2001

Arterial occlusion after surgery for ovarian malignancy. A case report.

BACKGROUND: Inherent in the risks of pelvic surgery is the postoperative morbidity and mortality associated with the procedure. The gynecologic oncology patient presents further risks for several reasons, including a relative state of hypercoagulability. Anticipation of the problem and early recognition are the keys to decreasing the long-term sequelae of a complication. A well-recognized complication of gynecologic oncology surgery is venous thromboembolism. Advances allow the prophylaxis, recognition and treatment of this problem. Acute arterial occlusion is a much less common but potentially devastating complication. CASE: A woman underwent surgery for ovarian malignancy and developed bilateral arterial occlusion of the lower extremities postoperatively. CONCLUSION: Understanding the various genetic defects that predispose certain individuals to hypercoagulability will help identify patients with a particularly high risk of developing acute arterial occlusive disease.

Aged↗
PubMed2000

High-throughput SNP genotyping with the Masscode system.

QIAGEN Genomics, Inc, has developed the Masscode tagging system for DNA labeling and detection. In this application, the Masscode system is described as applied to high-throughput single-nucleotide polymorphism (SNP) genotyping. The labeling system is based on a small-molecular-weight tag that is covalently attached through a photocleavable linker to a DNA oligonucleotide. The tagged oligonucleotide is used as a primer in an allele-specific PCR SNP discrimination assay. The allele-specific amplicons are differentiated through their Masscode tag assignments. After a photolysis step to cleave the tags from the amplicon, the samples are introduced into a single quadrupole mass spectrometry detection system for analysis. Genotyping determinations are based on the relative proportions of the paired allele tags. The system has a lower limit of detection in the femtomolar range (10(-15) M). At present, 30 different Masscode tags may be used simultaneously in a multiplex fashion to routinely provide more than 40,000 SNP genotyping measurements daily. Further developments will allow for the simultaneous detection of several hundred tags.

Alleles↗
PubMed2000

Hormonal therapy for sows weaned during fall and winter.

Sows from five commercial herds were weaned in the fall and winter and used to ascertain the potential application of hormonal therapy to stimulate subsequent litter size born. At weaning sows within parity (1, 2, and 3 through 6) and lactation length classification (early weaned, < or = 14 d; conventionally weaned, > 14 d) were randomly assigned to treatment. Treatments were injection with P.G. 600 (400 IU PMSG with 200 IU hCG) at weaning or no treatment. Sows were individually observed for estrus and mated following protocol for each herd. Breeding, culling, and farrowing data were collected. Treatment with P.G. 600 did not change subsequent rebreeding performance for sows at parity 1 or parities 3 through 6. However, conventionally weaned parity-2 sows treated with P.G. 600 were more likely (P < .05) to return to estrus than conventionally weaned controls (99.0 vs 93.6%, respectively). For parity-1 and parity-2 sows, treatment did not significantly change the likelihood for farrow percentage. However, the likelihood to farrow a litter was greater (P < .05) among P.G. 600-treated sows at parities 3 to 6 that were conventionally weaned (84.4 vs 71.3%, respectively). Subsequent litter birth weight for parity-1 sows treated with P.G. 600 was lower (P < .02) than for controls (15.6 vs 16.6 kg, respectively). Subsequent litter size at birth was not affected by treatment for parity-1 or parity-2 sows compared with controls. However, early-weaned sows at parities 3 through 6 treated with P.G. 600 had more (P < .06) total number born than controls (12.4 vs 10.6, respectively). Treatment with P.G. 600 improved reproductive function within specific parity and lactation length classification for sows weaned in the fall and winter.

Animals↗
PubMed1986

Health policy on the town meeting agenda.

American Health Decisions, a movement that started in Oregon, has spread to several other states. Through the media and widespread public discussion, the projects aim to increase public awareness about the individual and societal dimensions of ethical choices in health care.

Advisory Committees↗
PubMed1985

Oregon Health Decisions. An experiment with informed community consent.

Oregon Health Decisions is a citizen-based project intended to develop statewide awareness of severe bioethical dilemmas. The project has set in motion civic means for addressing and resolving problems in Oregon's health provision system associated with personal autonomy, equity of access, prevention of illness, and humane cost containment. The process of civic involvement with consequent results is described together with the implications for future health policy in Oregon and elsewhere.

Advisory Committees↗
PubMedDate not supplied

An overview of proteinase inhibitors.

Proteinase inhibitors are proteins in the body that regulate the catalytic activity of proteinases. They are important in a large variety of physiologic processes including coagulation, digestion, tumor metastasis and immunity. Proteinase inhibitors are categorized as either nonspecific proteinase inhibitors or class-specific proteinase inhibitors. Nonspecific proteinase inhibitors are comprised soley of the alpha macroglobulins, most notably alpha2-macroglobulin. Class-specific proteinase inhibitors are subcategorized as serine proteinase inhibitors, aspartic proteinase inhibitors, metalloproteinase inhibitors, and cysteine proteinase inhibitors. Each subcategory is made up of numerous inhibitors. As the roles of individual proteinase inhibitors are determined, the therapeutic use of natural and synthetic proteinase inhibitors is also being investigated. The purpose of this article is to review the history and classification of proteinase inhibitors and their relevance to veterinary medicine.

Animal Diseases↗