PubMed Health⌕ Search

Biomedical subjects

B Holstein

Publications and source records attributed to B Holstein.

28 records · Page 2Linked to original sources
PubMed1979

[Simultaneous determination of lysine, lysinoalanine, furosine, and pyridosine in food and feed (author's transl)].

A method for the simultaneous separation and determination of lysinoalanine (LAL), lysine, furosine and pyridosine with an automatic amino acid analyzer using a single Column 4 buffer- and 3 temperature-programme is described. The procedure needs 160 Minutes analysis time and allows a separation and quantitative determination of LAL, when present in amounts of more than 100 ppm (semiquantitative above 10 ppm). All amino acids found in food proteins and all other ninhydrinpositive compounds tested until now do not interfere with the determination.

Amino Acids↗
PubMed1979

Gastric acid secretion and water balance in the marine teleost Gadus morhua.

Gastric acid secretion was measured in unanaesthetized codfish surgically equipped with a catheter draining the stomach. The pylorus was ligated. Without perfusion of the intestine, or during perfusion with pure sea water (100% SW) or slightly diluted (67%) SW, gastric acid secretion was low ( less than 10 mumol H+/kg.h) and the fishes became dehydrated during the experiment. Perfusion of the intestine with 50% or 3% SW enabled the fishes to compensate for the water loss to the environment and greatly enhanced gastric acid secretion. Acid secretion was elevated also in fishes in which the dehydration was prevented by an intra-muscular infusion of saline. Perfusion of the intestine (8.5 ml/h) decreased the hourly effluent volume ('drinking rate') from the stomach to almost zero within 2 h. The effect was independent of the degree of dilution of the perfusing SW, but was less pronounced when the perfusion rate was lowered. Intra-muscular saline also depressed 'drinking rate'. The acid secretion prevailing during perfusion of the intestine with SW at a dilution preventing dehydration--suggested to represent the basal acid secretion of the codfish--was inhibited by atropine, hexamethonium, and metiamide.

Animals↗
PubMed1979

Gastric acid secretion and drinking in the Atlantic cod (Gadus morhua) during acidic or hyperosmotic perfusion of the intestine.

Cods were equipped with cannulae for drainage of the stomach and for separate perfusion of the stomach and intestine. Acidity, volume, and osmolality of the gastric outflow were measured. During perfusion of the intestine with a near-isosmotic saline (1 part sea-water, 2 parts distilled water, '33% SW') and the stomach with pure ('100%') SW, gastric acid output was high and volume output slightly above the infused volume. The osmolality of the gastric perfusate decreased during passage of the stomach. It was concluded that no drinking occurred, and that the decreased osmolality was due to dilution by gastric secretions and osmotically lost water. When substituting the isosmotic intestinal perfusion to a dehydrating perfusion (100% SW), gastric acid secretion was depressed but volume output was unaffected. Also perfusion of the intestine with acidified 33% SW depressed gastric acid secretion and in addition increased volume and osmolality of the gastric outflow. The results suggest that perfusion of the intestine depress the drinking reflex and that this depression is surmounted by intestinal acidification. Possible mediators of the intestinal feed-back mechanism for the inhibition of gastric acid secretion are discussed.

Animals↗
PubMed1977

Effect of atropine and SC-15396 on stimulated gastric acid secretion in the atlantic cod, Gadus morhua.

Gastric acid secretion was measured in swimming codfish surgically equipped with a catheter draining the stomach. Gastric acid secretion was stimulated by histamine (5 or 15 microgram/kg-h) or by carbachol (5 microgram/kg-h). Pretreatment with atropine (1 mumol/kg-h) completely prevented the acid secretion induced by carbachol, but did not influence the secretion induced by histamine. Atropine had marked effects on the motor functions of the stomach, and seriously reduced the volume draining from the stomach. Infusion of phenol red indicated that the decrease in volume was due to a decrease in recovery of ingested water. SC-15396, "antigastrin", significantly depressed acid secretion induced by histamine, and reduced carbachol-stimulated secretion, although the later was statistically insignificant. The effects of SC-15396 is discussed with reference to the absence of receptors for gastrin related to gastric acid secretion in the codfish stomach.

Acetamides↗
PubMed1977

Lung function and long-term exposure to cement dust.

In a cross-sectional epidemiological survey a selected group of former and present cement factory workers and a random sample of the corresponding urban population underwent dynamic spirometry. Based upon life experience the subjects were grouped into four occupational categories. Three hundred and one men were grouped as cement factory workers, 649 were grouped as blue collar workers, 218 as white collar workers and 102 men had predominantly been occupied in farming, forestry and fishing. On the average the investigated men had spent more than 75% of their total occupational life in their main occupational category. Each occupational category was further subdivided according to smoking habits and in all categories the well-known association between tobacco consumption and decrease in ventilatory capacity was found. White collar workers, who smoke less and had experienced better housing conditions throughout life had better ventilatory lung function than any of the other three occupational categories. No significant differences in lung function between cement factory workers and other blue collar workers with comparable smoking habits could be demonstrated by use of the maximal midexpiratory flow (MMEF), or any other of the calculated indices from the dynamic spirometry, when standardized for age, height and body weight.

Aged↗
PubMed1976

Effect of the H2-receptor antagonist metiamide on carbachol- and histamine-induced gastric acid secretion in the Atlantic cod,Gadus morhua.

Gastric acid secretion was measured in swimming codfish surgically equipped with a catheter draining the stomach. Intramuscular injection of histamine (15.6 mug/kg-h) or carbachol (5 mug/kg-h) stimulated the secretion of acid, carbachol in addition caused a marked decrease in drinking rate of the fishes. Administration of 10 mumol/kg-h metiamide completely prevented the acid secretion induced by both drugs. The carbachol effect on the drinking rate, interpreted to mirror contraction of the stomach following stimulation of muscarinic receptors, was not affected by metiamide.

Animals↗
PubMed1975

Gastric acid secretion in a teleostean fish: a method for the continous collection of gastric effluence from a swimming fish and its response to histamine and pentagastrin.

Gastric acid secretion has been measured in the codfish (Gadus morhua) equipped with a catheter draining the stomach, by titrating the water swallowed by the fish. Unstimulated acid secretion was found to be very low; in most fishes not exceeding 8 mumol H+/kg h. Intramuscular injection of histamine evokes a dose-dependent secretion of gastric acid, with a maximum acid output of 300 mumul/kg h occurring with 10 mg/kg histamine dihydrochloride. No acid response was obtained with pentagastrin.

Animals↗