The binding of metal ions by the outer membrane of Escherichia coli K-12 strain AB264 was investigated by using outer membrane obtained after Triton X-100 extraction of purified cell envelopes. Binding studies, conducted under saturating conditions, indicated a selective trapping of certain metallic ions. Low-dose electron microscopy of metal-loaded samples revealed an aggregative deposition of lead on one surface of the membrane which suggests that at least one distinctive binding site is asymmetrically arranged in these outer membrane vesicles.

Binding Sites↗

PubMed1980

Breast-feeding and food intake among children with acute diarrheal disease.

To quantitate reduced food intake during diarrhea and to assess possible means of promoting such intake, the 24-hr food and breast milk intakes of 41 children 6 to 35 months divided into three groups were measured. The energy and protein intake of 15 children hospitalized with acute watery diarrhea averaged 75 kcal/kg and 0.96 g/kg, respectively. The energy and protein consumption of another group of 15 children with diarrhea whose mothers received intensive education to promote food intake during hospitalization averaged 60.9 kcal/kg and 0.70 g/kg, respectively. These intake levels were significantly lower than 129.9 kcal/kg and 1.89 g/kg observed among healthy control children. These results suggest that child anorexia may be an important cause of reduced food intake during diarrhea. Anorexia was not overcome with intensive educational efforts. Breast milk was found to be important nutrient source with breast-fed children better protected against reduced intake during diarrhea.

Acute Disease↗

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