Biomedical subjects

B Humphries

Publications and source records attributed to B Humphries.

12 recordsLinked to original sources

PubMed2002

The effects of timing and application of vibration on muscular contractions.

BACKGROUND: The effect of vibration stimulation on muscular strength is an emerging field of research and very little comprehensive work has been conducted at this stage. HYPOTHESIS: There will be no effects of timing or application of vibration stimulation on muscular strength and activation across isometric, isokinetic and concentric isotonic contractions. METHODS: There were 28 recreational athletes who participated in this study. Their characteristics were: (mean +/- SD) age, 22.8 +/- 5.6 yr; height, 174.1 +/- 8.8 cm; and body mass, 78.0 +/- 13.6 kg. The vibration stimulation was delivered at 50.42 +/- 1.16 Hz with an acceleration of 13.24 +/- 0.18 ms(-2). RESULTS: A series of one-way ANOVAs revealed significant (p < 0.05) improvements of 14.7 +/- 2.9% and 15.3 +/- 3.1% above normal contraction levels for concentric isotonic strength during and after the vibration stimulation, respectively. No significant improvements in isometric and isokinetic strength were evident. Concurrent measurement of electromyography (EMG) presented significant improvements during stimulation of 30.1 +/- 14.6%, 43.0 +/- 13.0%, and 107.1 +/- 44.4% in mean activation of rectus femoris (RF) for the isometric, isokinetic, and concentric isotonic contractions, respectively. Synchronous collection of vibromyography (VMG) during stimulation displayed a significant decrease of -6.4 +/- 1.5%, -5.1 +/- 1.2%, and -4.1 +/- 1.7% in mean VMG activity of RF for the isometric, isokinetic, and concentric isotonic contractions, respectively. CONCLUSIONS: Significant improvements in muscular strength and activation for concentric isotonic contractions performed during an applied vibration suggest that the optimal timing of a vibratory stimulation would be while the participant is contracting isotonically. However, further research needs to be conducted to establish the exact mechanism behind these improvements.

Adolescent↗

PubMed2001

Identifying bone mass and muscular changes.

The aim of this investigation was to examine the impact of a six-month high intensity strength-training program on lumbar bone mineral density (BMD), trunk and lower limb strength in a population of Australian women aged 50 years and over. A subject pool of 44 women were recruited and randomly allocated into either strength training (n = 19) or active control (n = 25) groups. All subjects trained twice weekly in either a 50 minute supervised strength training session that progressed from 60% one repetition maximum (1RM) to 90% 1RM or a 50 minute group walk session. Measurements included a lumbar (L2-L4) BMD scan: peak isokinetic trunk strength and a dynamic 1RM squat as a measure of lower body strength. No significant group differences in lumbar BMD were evident at the completion of training. However, a significant (p < 0.05) within group change was apparent for the active control group as lumbar BMD decreased 1.7% below baseline testing. A significant (p < 0.05) group difference was evident with the strength trained group increasing peak isokinetic trunk strength (19.3%) and 1RM squat strength (34.4%) above that of the active controls. It was concluded that strength training provides an effective means for increasing trunk and lower limb strength in women over 50 years. The impact of strength training on lumbar BMD was not conclusive in the present study.

Absorptiometry, Photon↗

PubMed2000

Kinanthropometric and physiological characteristics of outrigger canoe paddlers.

We describe the physiological characteristics of amateur outrigger canoe paddlers. Twenty-one paddlers (13 males, 8 females) were evaluated for body stature, aerobic power, muscular strength and endurance, peak paddle force, flexibility and 250 m sprint paddle performance at the end of the outrigging season. The mean variables (+/- s) for the males were: age 27 +/- 9 years, height 175 +/- 5 cm, body mass 80 +/- 5 kg, arm span 178 +/- 7 cm, sitting height 100 +/- 2 cm, aerobic power 3.0 +/- 0.4 l x min(-1), maximum bench press strength 85 +/- 19 kg, right peak paddle force 382 +/- 66 N and left peak paddle force 369 +/- 69 N. For the females, these were: age 26 +/- 6 years, height 168 +/- 5 cm, body mass 70 +/- 8 kg, arm span 170 +/- 5 cm, sitting height 97 +/- 3 cm, aerobic power 2.3 +/- 0.51 l x min(-1), maximum bench press strength 47 +/- 10 kg, right peak paddle force 252 +/- 63 N and left peak paddle force 257 +/- 60 N. Analysis of variance revealed differences (P < 0.05) between the dominant and non-dominant sides of the body for peak paddle force, isokinetic internal and external rotation, and flexion and extension torque of the shoulder joint. The outrigger canoe paddlers were generally within the range of scores found to describe participants of other water craft sports. Outrigger canoeists should be concerned with the muscular strength imbalances associated with paddling technique.

Adult↗

PubMed2000

Effect of exercise intensity on bone density, strength, and calcium turnover in older women.

PURPOSE: This study examined the effects of 24 wk of high intensity strength training or low intensity walking on lumbar bone mineral density (BMD), muscular strength, and calcium turnover in Australian women either taking hormone replacement therapy (HRT) or not taking HRT. METHODS: A subject pool of 64 women between 45-65 yr and randomly allocated into weights (N = 21), walking (N = 20), weightsHRT (N = 14), and walkingHRT (N = 9) groups completed this study. All subjects trained twice weekly in either a 50-min walking or weight-training program (60-90% IRM). Measurements included maximal isometric knee strength, IRM bench press, IRM squat, isokinetic back strength, lumbar (L2-L4) BMD, serum osteocalcin, and urinary deoxypyridinoline crosslinks (Dpd). RESULTS: No significant group differences in BMD were evident at the completion of training. However, a significant (P < 0.05) within group change was apparent for the walking group since BMD decreased 1.3% below baseline testing. Osteocalcin levels increased significantly (P < 0.05) in the walking (22%) group. Maximal bench press and squat strength improved significantly (P < 0.05) in the weights (25.8% and 37.7%) and weightsHRT (25.4% and 35.7%) groups. The weights group also increased significantly (P < 0.05) in isokinetic back strength (22.2%). CONCLUSION: It was concluded that short-term high intensity resistance training provides an effective means for increasing muscular strength in women between 45 and 65 yr. The training effects on lumbar BMD were not apparent in the present study.

Aged↗

PubMed1999

The relationship between dynamic, isokinetic and isometric strength and bone mineral density in a population of 45 to 65 year old women.

This study investigated the relationship between age, lumbar spine bone mineral density (LS BMD) and muscular strength of peri and postmenopausal women between 45 and 65 years either taking or not taking hormone replacement therapy (HRT). Ninety six women were tested for LS BMD (L2-L4), one repetition maximum (1RM) bench press and squat, maximal voluntary isometric contraction (MVC) of the knee extensors and peak torque of back extensor muscles at a speed of 30 degrees s(-1). Bone and strength data were analysed to evaluate the relationships in incrementing five year age groups and based on groups either taking or not taking HRT. ANOVA revealed significant differences in LS BMD between the 45-49 and 55-59 (F[3,92]=2.6411, p<0.05; -8%) age groups amounting to an annual bone loss of 0.8% for this Australian based population. Non significant LS BMD results were evident after controlling for the influence of age and menopausal status on the groups either taking or not taking HRT. Significant differences between the 45-49 and 60-64 (F[3,92]=2.7463, p<0.05) age groups for 1RM bench press and the 45-49 and 60-64, 50-54 and 60-64, and, 55-59 and 60-64 (F[3,92]=4.2816, p<0.05) age groups for 1RM squat amounting to an 18.8% and 37.5% loss of dynamic upper and lower body strength, respectively. Group correlation coefficients ranged between (r=-0.20 and -0.34, p<0.05) for LS BMD, strength and age. The conclusions demonstrate a concomitant decline in maximal muscle strength and bone density between women 45 and 65 years irrespective of HRT. These results also demonstrate a 50% greater decline in lower body strength compared to upper body strength between women 45 and 65 years.

Absorptiometry, Photon↗

PubMed1997

Myoelectric evidence of peripheral muscle fatigue during exercise in severe hypoxia: some references to m. vastus lateralis myosin heavy chain composition.

Integrated electromyography (iEMG) of the m. vastus lateralis was analysed during cycle ergometry in male subjects (n = 8). Two work trials were conducted, one under normoxia (N), the other under environmental normobaric hypoxia (EH in which the oxygen fraction in inspired gas = 0.116), each trial lasting 10 min. The absolute power output (180 W) was the same for both trials and was equivalent to 77 (4)% of maximum heart rate in trial N. Maximal voluntary isometric contractions were performed after each trial to assess changes in force, muscle fibre conduction velocity (MFCV), electromechanical delay (EMD), median frequency of EMG (MF) and maximal iEMG (iEMGmax). Biopsy samples of muscle were obtained from the m. vastus medialis before testing. Myosin heavy chain (MHC) differences were determined through sodium dodecyl-polyacrylamide gel electrophoresis followed by densitometric analysis. No differences in submaximal iEMG were observed between EH and N trials during the first minute of work. At the end of both work trials iEMG was significantly elevated compared with starting values, however the iEMG recorded in EH exceeded N values by 15%. At the end of the EH trials the following were observed: a decrease in isometric force, MFCV and MF with an increase in EMD and the iEMGmax/force ratio. The iEMGmax was unchanged. No differences in any of these variables were observed after the N trial. Mean (SD) lactate concentrations following EH and N trials were 9.2 (4.4) mmol x 1(-1) and 3.5 (1.1) mmol x 1(-1), respectively. Results indicate that an increased motor unit recruitment and rate coding was needed in EH to maintain the required power output. The increased motor unit recruitment and rate coding were associated with myoelectric evidence of "peripheral" muscle fatigue. Subjects with higher compositions of type II MHC accumulated more lactate and displayed greater reductions in MF and MFCV during fatigue.

Adult↗

PubMed1997

Electrophoretic separation of myosin heavy chain isoforms in the human m. vastus lateralis: references to reproducibility and relationships with force, electromechanical delay, fibre conduction velocity, endurance and electromyography.

The aim of this investigation was to determine the relationship between muscle performance and the myosin heavy chain (MHC) composition and the reliability of electrophoretically determined MHC compositions. A total of thirty-one male subjects participated in the experiments. Maximal voluntary isometric contractions (MVC) of the knee extensors were performed at an arbitrary knee angle of 90 degrees and the following variables were recorded: maximal isometric force, muscle fibre conduction velocity (MFCV), electromechanical delay (EMD), maximal rate of force development (MRFD), median frequency of EMG (MF) and iEMG. Static isometric contractions of the knee extensors were held at an angle of 90 degrees using contractile forces of 10%, 50% and 100% MVC, respectively. These tests were conducted on separate days. Muscle biopsy samples were obtained from the left m. vastus lateralis before MVC and static endurance tests. MHC protein isoform differences were determined through sodium dodecyl-polyacrylamide gel electrophoresis (SDS-PAGE) followed by densitometric analysis. Type I-MHC compositions of the m. vastus lateralis ranged from 20-68% with a mean of 49 +/- 18%, mean type IIa-MHC and type IIb-MHC percentages were 35 +/- 16% and 16 +/- 10%, respectively. MHC compositions of duplicate biopsy samples were not significantly different from that of original samples. The coefficients of variation calculated for duplicate biopsy samples suggested reasonable reproducibility for MHC isoform differentiation for type I-MHC and type-II MHC composition (CV = 12.6%). Differentiation between type IIa-MHC and type IIb-MHC was not always clear using the densitometric traces. Subjects with higher percentages of type II-MHC displayed significantly faster MFCV (r = 0.67, P < 0.1), isometric force development (r = 0.68, P < 0.1) and shorter periods of EMD (r = -0.72, P < 0.05). There was also a tendency toward faster MRFD in these subjects although results did not reach significance. Endurance times for isometric contractions held at 10%, 50% and 100% MVC to exhaustion were not correlated with MHC composition. No relationships between II-MHC composition and MF or iEMG were observed. It was suggested that surface electromyographic recordings obtained during isometric MVC did not reflect underlying differences in muscle fibre composition.

Adult↗

PubMed1992

Persistent pulmonary hypertension complicating cystic adenomatoid malformation in neonates.

Neonates with congenital diaphragmatic hernia (CDH) are known to be susceptible to stress-induced persistent pulmonary hypertension (PPHN). Congenital cystic adenomatoid malformations (CCAMs) may also present as respiratory distress in the newborn. Intubation and mechanical ventilation cause clinical deterioration because of air trapping within cystic spaces; these patients require prompt lobectomy. PPHN has not been commonly associated with CCAM. Three patients with CCAM were encountered who developed PPHN postlobectomy. Three newborns, 36 to 38 weeks' gestation, presented with respiratory distress. Two had diagnosis of thoracic tumors on fetal ultrasound (22 and 33 weeks). Chest x-ray at birth confirmed cystic intrathoracic tumors in all and they underwent immediate thoracotomy and lobectomy (1 right upper, 1 left lower, 1 left upper). The patients were stable for 4 hours to 5 days postoperatively and then developed findings consistent with PPHN by cardiac echocardiography and required extracorporeal membrane oxygen (ECMO) support. ECMO was required for 66.5 to 120 hours. Each patient was successfully weaned to conventional ventilatory support. The clinical course of these patients was similar to those with CDH who undergo immediate surgery. The stress of surgical intervention combined with hypoxia and hypercarbia stimulates a hyperactive pulmonary vasculature and the development of PPHN. ECMO provides an effective adjunct to support patients with PPHN on the basis of congenital cystic adenomatoid malformations.

Cystic Adenomatoid Malformation of Lung, Congenita↗

PubMed1992

Major surgical intervention during extracorporeal membrane oxygenation.

Of 135 patients treated with extracorporeal membrane oxygenation (ECMO) between January 1987 and December 1989, 19 (14.0%) patients underwent surgical procedures while on ECMO. Thirteen (68%) patients had operations related to hemorrhage, including cannula site (6), mediastinal (1), hemoperitoneum (3), and hemothorax (3). Six of 13 patients required repetitive operations for bleeding; 4 of 6 died. Six (35%) patients had operations for congenital pathology including patent ductus (PDA) ligation (2), repair of transposition of the great vessels (2), repair of coarctation (1), and repair of congenital diaphragmatic hernia (3). One patient had multiple simultaneous procedures performed. Of these 6 patients, 4 were decannulated immediately and 2 were decannulated within 28 hours following surgery without any bleeding complications. Fifteen of 19 patients were operated on in the neonatal intensive care unit. The 4 remaining patients required transport on ECMO to the surgical suite. Thirteen of the 19 patients requiring surgical intervention on ECMO survived. In the 13 survivors, the mean time to decannulation postoperative was 45 hours, and in those that died it was 90 hours. Our experience suggests that surgical intervention while on ECMO is technically feasible with the best results achieved when rapid discontinuation of ECMO can be accomplished postoperatively. Due to this fact major surgical intervention should be postponed if possible until near the conclusion of the ECMO therapy.

Algorithms↗

PubMed1992

Extracorporeal membrane oxygenation in lambs through umbilical vessel perfusion: cardiac and hepatic complications.

Twin lambs were delivered by ceasarean section near term, aralyzed, sedated and randomly assigned to either mechanical ventilation or umbilical arteriovenous ECMO for 48 hours. Umbilical arteriovenous ECMO provided adequate gas exchange with minimal or no ventilation of the native lungs. However, at autopsy, animals treated with umbilical ECMO showed right heart dilation and liver necrosis or hemorrhage compared to their twins treated with mechanical ventilation.

Animals↗

PubMed1991

The impact of extracorporeal membrane support in the treatment of congenital diaphragmatic hernia.

Neonates with congenital diaphragmatic hernia (CDH) treated by immediate surgical intervention and conventional ventilatory support have an overall poor survival. The potential of extracorporeal membrane oxygenation (ECMO) therapy to improve survival of infants with CDH remains controversial. Comparison was made in a single institution's pre-ECMO and post-ECMO survival statistics to establish efficacy of extracorporeal support for persistent pulmonary hypertension (PPH). This study was accomplished by stratifying patients by an oxygen index (OI). Sixty-eight patients were treated for CDH from 1977 to 1986 without ECMO. Fifty-eight patients underwent repair of CDH within the first 24 hours of life. Data could be retrieved for calculation of the OI in 46 patients. Nineteen patients developed an OI of 40 or greater; one survived (5%). Three of 27 patients with an OI less than 40 died (OIs = 34, 38, and 38). Thirty-one patients were treated from 1987 to 1989 and none were excluded from ECMO based on a minimum PO2. Fifteen had an OI less than 40 (range, 1 to 38), were treated conventionally, and 13 survived (87%). Sixteen patients had an OI greater than 40 and 13 qualified for ECMO. Nine of 13 survived (69%). Comparing pre-ECMO and post-ECMO survival for infants with an OI of 40 or greater (5% v 69%), there is a significant improvement in survival when ECMO is used (P less than .001). ECMO support offers a strong adjunct in management of neonates with CDH who develop PPH.

Extracorporeal Membrane Oxygenation↗

PubMedDate not supplied

1,1,1-trichloro-2,2-bis (p-chlorophenyl)-ethane (DDT) and reduced bone mineral density.

The organochlorine pesticide 1,1,1-trichloro-2,2-bis (p-chlorophenyl)-ethane (DDT), is a well-known and widely dispersed "environmental estrogen" (World Health Organization Criteria no. 9; Geneva, Switzerland [1979]). Kelce et al. (Nature, 1995; 375:581-85) recently identified the DDT metabolite, 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), has also recently been identified as a potent androgen receptor antagonist. The authors examined the relationship between serum levels of DDE and bone mineral density in 68 sedentary women who reported adequate dietary intake of calcium. Reduced bone mineral density was correlated significantly with age (r = -.36, p = .004), as well as with increases in the log of DDE levels in serum (r = -.27, p = .03). The authors also used multiple-regression analysis to examine the influence of other predictor variables on the relationship between log DDE and bone mineral density. The strongest model (p = .002) included log DDE (p = .018), age (p = .002), and years on hormone replacement therapy (p = .10) as predictor variables, and this model afforded prediction of 21% of bone mineral density variation. These results suggest that past community exposures to DDT may be associated with reduced bone mineral density in women.

Absorptiometry, Photon↗