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Biomedical subjects

B Huttner

Publications and source records attributed to B Huttner.

17 recordsLinked to original sources

[Infectious spondylitis].

Infectious spondylitis usually involves osteomyelitis in two adjacent vertebral bodies and the intervertebral disc (spondylodiscitis). The most common location is the lumbar spine, followed by the thoracic spine. Symptoms are nonspecific, leading to a delay in diagnosis, in many cases, of several weeks. A large number of infectious agents can cause vertebral osteomyelitis, usually reaching the vertebra by hematogenous spread. The most commonly isolated agent is Staphylococcus aureus. Spondylitis remains the most common skeletal manifestation of tuberculosis. As with other forms of osteomyelitis, microbiological diagnosis is essential for the choice of adequate therapy. The majority of cases can be cured with antibiotic therapy alone.

Anti-Bacterial Agents↗

A PEST-like element in FREQUENCY determines the length of the circadian period in Neurospora crassa.

FREQUENCY (FRQ) is a crucial element of the circadian clock in Neurospora crassa. In the course of a circadian day FRQ is successively phosphorylated and degraded. Here we report that two PEST-like elements in FRQ, PEST-1 and PEST-2, are phosphorylated in vitro by recombinant CK-1a and CK-1b, two newly identified Neurospora homologs of casein kinase 1 epsilon. CK-1a is localized in the cytosol and the nuclei of Neurospora and it is in a complex with FRQ in vivo. Deletion of PEST-1 results in hypophosphorylation of FRQ and causes significantly increased protein stability. A strain harboring the mutant frq Delta PEST-1 gene shows no rhythmic conidiation. Despite the lack of overt rhythmicity, frq Delta PEST-1 RNA and FRQ Delta PEST-1 protein are rhythmically expressed and oscillate in constant darkness with a circadian period of 28 h. Thus, by deletion of PEST-1 the circadian period is lengthened and overt rhythmicity is dissociated from molecular oscillations of clock components.

Amino Acid Sequence↗