Biomedical subjects

B J Deeb

Publications and source records attributed to B J Deeb.

21 records · Page 2Linked to original sources

PubMed1985

Toxoplasma gondii infection of cats in Beirut, Lebanon.

The prevalence of serum antibodies to Toxoplasma gondii was determined by the indirect fluorescent antibody (IFA) test in cats of Beirut, Lebanon from September 1980 to July 1983 (35 months). Testing revealed that 253/324 (78.1%) cats had antibodies in the IFA test. While the prevalence of Toxoplasma antibodies in stray cats was considerably greater than in owned cats at the beginning of the study, this difference diminished over time, and prevalence was similar by the end of the study. Faecal shedding of Toxoplasma-like oocysts was found in 31/313 (9.9%) of cats, while 38/313 (12.1%) shed other Isospora spp. oocysts. The occurrence of dual infections was statistically significant.

Animals↗

PubMed1967

Characterization of Mycoplasma pulmonis variants isolated from rabbits. I. Identification and properties of isolates.

Mycoplasma showing at least two colony types were isolated from the nares and oropharynx of New Zealand white rabbits. Two strains were purified by single-colony passages and characterized. Morphology by phase-contrast and electron microscopy was typical of Mycoplasmataceae. Both grew anaerobically as well as aerobically, caused hemolysis of guinea pig, sheep, and horse red blood cells, and fermented glucose. These characteristics are shared by members of the species M. pulmonis, commonly isolated from the respiratory tracts of laboratory rats and mice. By use of the growth-inhibition test and agar-gel double-diffusion tests, the two strains were found to be serologically related to each other and to M. pulmonis ATCC 14267 but not to other representative Mycoplasma species from man and animals.

Animals↗

PubMed1967

Characterizion of Mycoplasma pulmonis variants isolated from rabbits. II. Basis for differentiation of antigenic subtypes.

The antigen composition of Mycoplasma pulmonis variants was studied by complement-fixation, agar-gel diffusion, and growth-inhibition tests. Two classes of complement-fixing antigens were demonstrated for M. pulmonis strains 47 and 63: (i) cross-related, heat-labile, water-soluble antigens, and (ii) high-titered, subtype-specific, heat-stable, water-soluble antigens. Lipid antigens prepared by organic solvent fractionation were low-titered antigens and showed little specificity. With the aid of agar-gel double-diffusion plates, the subtype-specific antigens were found to be precipitated by trichloroacetic acid and to be stable to periodate, but they were inactivated by pronase. Pronase-stable, periodate-labile precipitating antigens were observed as common components between the two variants. Antisera prepared with boiled antigens were found to be serologically active on gel diffusion but lacked neutralizing ability in growth-inhibition tests. Each of three strains of M. pulmonis (47, 63, ATCC 14267) could be identified as a variant because each strain possessed immunologically distinct heat-stable subtype-specific antigen(s).

Antigens↗