Biomedical subjects

B J Ducro

Publications and source records attributed to B J Ducro.

12 recordsLinked to original sources

PubMed2006

Optimization of dairy cattle breeding programs for different environments with genotype by environment interaction.

Dairy cattle breeding organizations tend to sell semen to breeders operating in different environments and genotype x environment interaction may play a role. The objective of this study was to investigate optimization of dairy cattle breeding programs for 2 environments with genotype x environment interaction. Breeding strategies differed in 1) including 1 or 2 environments in the breeding goal, 2) running either 1 or 2 breeding programs, and 3) progeny testing bulls in 1 or 2 environments. Breeding strategies were evaluated on average genetic gain of both environments, which was predicted by using a pseudo-BLUP selection index model. When both environments were equally important and the genetic correlation was higher than 0.61, the highest average genetic gain was achieved with a single breeding program with progeny-testing all bulls in both environments. When the genetic correlation was lower than 0.61, it was optimal to have 2 environment-specific breeding programs progeny-testing an equal number of bulls in their own environment only. Breeding strategies differed by 2 to 12% in average genetic gain, when the genetic correlation ranged between 0.50 and 1.00. Ranking of breeding strategies, based on the highest average genetic gain, was relatively insensitive to heritability, number of progeny per bull, and the relative importance of both environments, but was very sensitive to selection intensity. With more intense selection, running 2 environment-specific breeding programs was optimal for genetic correlations up to 0.70-0.80, but this strategy was less appropriate for situations where 1 of the 2 environments had a relative importance less than 10 to 20%. Results of this study can be used as guidelines to optimize breeding programs when breeding dairy cattle for different parts of the world.

Animals↗

PubMed2005

Selection strategies for body weight and reduced ascites susceptibility in broilers.

Ascites syndrome is a metabolic disorder in broilers. Mortality due to ascites results in significant economic losses and has a negative impact on animal welfare. It has been shown that genetic factors play a considerable role in susceptibility of birds to ascites, which offers perspectives for selection against this syndrome. The aim of the present study was to evaluate the consequences of alternative selection strategies for BW and resistance to ascites syndrome using deterministic simulation. In addition to the consequences of current selection (i.e., selection for increased BW only) alternative selection strategies including information on different ascites-related traits measured under normal or cold conditions and the consequences of having information on the underlying genes (i.e., MAS) were quantified. Five different breeding schemes were compared based on the selection response for BW, ascites susceptibility, and the rate of inbreeding. Traits investigated in the index as indicators for ascites were hematocrit value (HCT) and ratio of right ventricle to the total ventricular weight of the heart (RV:TV). The results indicated that by ignoring ascites susceptibility in the breeding goal, the gain for BW is 130 g and the birds will become more susceptible to ascites. Testing 50% of the birds under cold temperature conditions and including information of ascites related traits (HCT and RV:TV) measured under normal and cold conditions makes it possible to achieve a relatively high gain for BW (111.4 g) while controlling the genetic level for ascites susceptibility (selection response was 0). The results of scenarios including QTL information of ascites susceptibility showed that QTL information could be used very effectively in controlling ascites susceptibility.

Animals↗

PubMed2005

Genetic parameters for carcass composition and pork quality estimated in a commercial production chain.

Breeding goals in pigs are subject to change and are directed much more toward retail carcass yield and meat quality because of the high economic value of these traits. The objective of this study was to estimate genetic parameters of growth, carcass, and meat quality traits. Carcass components included ham and loin weights as primal cuts, which were further dissected into boneless subprimal cuts. Meat quality traits included pH, drip loss, purge, firmness, and color and marbling of both ham and loin. Phenotypic measurements were collected on a commercial crossbred pig population (n = 1,855). Genetic parameters were estimated using REML procedures applied to a bivariate animal model. Heritability estimates for carcass traits varied from 0.29 to 0.51, with 0.39 and 0.51 for the boneless subprimals of ham and loin, respectively. Heritability estimates for meat quality traits ranged from 0.08 to 0.28, with low estimates for the water holding capacity traits and higher values for the color traits: Minolta b*(0.14), L* (0.15), a* (0.24), and Japanese color scale (0.25). Heritability estimates differed for marbling of ham (0.14) and loin (0.31). Neither backfat nor ADG was correlated with loin depth (r(g) = 0.0), and their mutual genetic correlation was 0.27. Loin primal was moderately correlated with ham primal (r(g) = 0.31) and more strongly correlated with boneless ham (r(g) = 0.58). Backfat was negatively correlated with (sub)primal cut values. Average daily gain was unfavorably correlated with subprimals and with most meat quality characteristics measured. Genetic correlations among the color measurements and water-holding capacity traits were high (average r(g) = 0.70), except for Minolta a* (average r(g) = 0.17). The estimated genetic parameters indicate that meat quality and valuable cut yields can be improved by genetic selection. The estimated genetic parameters make it possible to predict the response to selection on performance, carcass, and meat quality traits and to design an effective breeding strategy fitting pricing systems based on retail carcass and quality characteristics.

Adipose Tissue↗

PubMed2004

Genetic and phenotypic correlations between feather pecking behavior, stress response, immune response, and egg quality traits in laying hens.

The objective of the current study was to estimate genetic and phenotypic correlations among feather pecking (FP) behavior and stress response, immune response, and egg quality parameters. These traits have been measured in an F2 cross, coming from a cross between a high and a low FP line of laying hens. Heritabilities (h2) of stress response (32 wk), primary immune response to keyhole limpet hemocyanin (KLH) (36 wk) and Mycobacterium butyricum (39 wk), and egg quality parameters (35, 44, and 50 wk of age) were calculated. The h2 was 0.05 +/- 0.05 (SE) for stress response, 0.15 +/- 0.07 for antibody response to KLH, and 0.08 +/- 0.06 for antibody response to M. butyricum. The h2 for egg quality traits were in the range of 0.12 to 0.30. Significant phenotypic correlations were found between gentle FP in adult hens and stress response, egg weight at 44 and 50 wk, and egg deformation at 50 wk. Significant additive genetic correlations were found between severe FP in adult hens and antibody response to KLH (0.79 +/- 0.35), and between ground pecking in adult hens and egg deformation at 50 wk (0.63 +/- 0.26), and between ground pecking and eggshell strength at 35, 44, and 50 wk of age (-0.86 +/- 0.29, -0.81 +/- 0.20, -0.76 +/- 0.24, respectively).

Aging↗

PubMed2004

Rapid chilling cannot prevent inferior pork quality caused by high preslaughter stress.

The present experiment investigated whether increasing chilling rate could improve meat quality in pigs exposed to either minimal or high stress immediately preslaughter. Pigs (n = 192) were offspring of halothane-free lines. On various days, four groups of 48 pigs were processed at a commercial plant. Within each group, half the pigs were exposed to either minimal or high preslaughter stress. Before entering the cooler at 45 min postmortem, carcasses of both minimal and high preslaughter stress treatments were allocated randomly to either conventional (+4 degrees C for 22 h) or rapid (three-phase chilling tunnel: -15, -10, and -1 degrees C for 15, 38, and 38 min, respectively, followed by storage at 4 degrees C until 22 h postmortem) chilling. Temperature and pH were measured in the blood at exsanguination and in the longissimus lumborum (LL) and semimembranosus (SM) muscle at 0.5, 2.5, 4.5, 6.5, and 22 h postmortem. Meat quality attributes (water-holding capacity and objective color measurements) were assessed on the LL. Preslaughter stress level affected pH and temperature in both blood and muscle, with lower (P < 0.001) pH values and higher (P < 0.001) temperatures for pigs exposed to high vs. minimal stress. Rapid chilling led to a faster (P < 0.001) temperature decline regardless of preslaughter stress level. Rapid chilling did not (P > 0.05) influence the rate of pH decline in the LL muscle, but reduced (P = 0.061) pH decline in the SM. Rapid chilling, as opposed to conventional chilling, decreased (P < 0.05) electrical conductivity in the LL, regardless of preslaughter stress; however, it could not compensate for the detrimental effect (P < 0.05) of stress on drip loss, filter paper moisture absorption, and meat color (L* value). Results from the present study indicated that increasing chilling rate is not a suitable method to resolve pork quality problems caused by inadequate preslaughter handling.

Animals↗

PubMed2004

Preslaughter stress and muscle energy largely determine pork quality at two commercial processing plants.

The objective of the present experiment was to study physiological changes elicited in response to stress in the immediate preslaughter period and to link them to pork quality characteristics. Crossbred, halothane-free pigs (n = 192) were processed in eight groups (24 pigs per group) on various days at one of two commercial processing plants operating different stunning systems (electrical and CO2 stunning in Plants A and B, respectively). In each group, half the pigs were exposed to either minimal or high preslaughter stress. Blood samples were taken at exsanguination, and lactate, cortisol, and catecholamines, as well as blood pH and temperature, were assessed and linked to various longissimus muscle quality attributes. Additionally, muscle pH and temperature were measured 30 min postmortem, and muscle glycolytic potential was determined 22 h postmortem. At both processing plants, high preslaughter stress resulted in higher (P < 0.05) blood cortisol and lactate; however, the effects of preslaughter stress on catecholamines and blood pH were believed to be biased by the different stunning methods employed at the plants. High preslaughter stress increased (P < 0.05) blood temperature at Plant A but not at Plant B. At both plants, high stress increased (P < 0.05) 30-min muscle temperature and decreased (P < 0.05) 30-min muscle pH. Ultimate pH was increased (P < 0.05) and muscle glycolytic potential was decreased (P < 0.05) by high preslaughter stress. At both plants, high stress resulted in inferior pork quality attributes (P < 0.05), including reflectance, electrical conductivity, filter paper moisture, drip loss, and L* value. The effect of stress was greater on water-holding capacity than on pork color, with drip losses increased by 56%. Of all stress indicators measured at exsanguination, only blood lactate was strongly correlated with pork quality attributes. Regression analyses revealed that blood lactate and glycolytic potential accounted for 52 and 48% of the variation in drip loss and L* value, respectively. In combination with high preslaughter stress, high glycolytic potentials were related to increased drip losses. We conclude that high preslaughter stress leads to impaired pork quality, with high muscle energy levels aggravating the negative effects of preslaughter stress. Monitoring stress level by blood lactate measurement in combination with strategies to control muscle energy present at slaughter may help to improve meat quality.

Animals↗

PubMed2001

Evaluation of between-line variation for within-line selection against ascites in broilers.

Within-line opportunities for selection against ascites were studied in a data set comprising a 10-line comparison. The study attempted to reveal whether contrasts between lines provide reliable candidate traits for within-line selection. Mortality was chosen as the reference trait. As no pedigree information was available, a trait was required that related mortality to the nonmortality data. By principal component analysis, such a trait (ASC_INDIC = ascites indicator) was developed from pathology data. The composite trait ASC_INDIC ranked lines well for their mortality figures (r = 0.96), from which it was concluded that ASC_INDIC represents an underlying continuous ascites trait. Between lines, blood gas traits seemed to be the most promising traits. Within lines, performance traits appeared to be highly correlated to ascites. Comparison of within-line variation to between-line contrasts revealed considerable differences. The high correlation of the blood gas traits with mortality was not present within lines. However, although the magnitude was considerably reduced, the nature of the blood gas traits in their relationship to ascites was similar within and between lines. The study primarily demonstrates that contrasts between lines carry systematic but limited information for within-line coherence. Therefore, line contrasts must be interpreted with care when aiming to study genetic variation and coherence within lines.

Animal Husbandry↗

PubMed2001

Genetic parameters for cystic ovarian disease in dutch black and white dairy cattle.

Cystic ovarian disease (COD) is one of the most frequently diagnosed gynecological findings in dairy cattle. It causes temporary infertility and is likely to affect reproduction as well as production parameters in cows. The objectives of this study were to investigate the heritability of COD in a Dutch Black and White population and to estimate the genetic and phenotypic relationships with milk production traits. In the data set used, the overall incidence of COD was 7.7% (1204 COD diagnoses in 15,562 lactations). The farm incidence varied between 1.9 and 11.3%. The estimated heritabilities on the underlying and observable scales were 0.102 and 0.087, respectively. The genetic correlations between COD and 305-d milk, fat, and protein yields were 0.345, 0.379, and 0.441, respectively. We concluded that a genetic predisposition for COD exists in Dutch Black and White dairy cattle. The genetic correlations between COD and yield traits indicate that ongoing selection for production will increase the incidence of COD.

Animals↗

PubMed1998

Diphasic allometric growth of some skeletal bones and the digestive tract in White Leghorn pullets consuming ad libitum and restricted diets.

Growth data of some bones (shank, tibia, and keel) and the digestive tract in White Leghorn pullets, which consumed ad libitum and restricted diets, were analyzed by mono- and diphasic allometric functions. Fat-free plucked empty body mass (FFEBM) or a functional entity was used as the independent variable in the equations. Pullets had been fed a low-lysine diet or a daily restricted amount of an adequate diet, from 0 to 6 or 7 to 18 wk of age. An additional group of pullets consumed ad libitum a control starter and grower diet. Relative growth of the skeletal bones and parts of the digestive tract, vs FFEBM, was described most accurately by a diphasic model. For each constituent, allometric slopes of the first growth phase (beta1) vs FFEBM were smaller than 1 (beta1 varied from 0.39 to 0.43 for shank and tibia, from 0.48 to 0.73 for the keel, from 0.89 to 0.98 for the total digestive tract, and from 0.80 to 0.84 for the gizzard, separately). These results suggest that each of the assessed organs matured earlier than the FFEBM. Except for the keel, which grew relatively faster than FFEBM if an early nutrient restriction had been applied, beta1 of all other assessed structures was similar for all treatments. If the allometric slope of the second growth phase (beta2) was estimated to be not different from zero, then the breakpoint between both phases was defined as the moment of attainment of maturity for the respective constituent. The attainment of maturity of the different body structures confirmed the classical growth sequence studies of the Hammond School. No differences in mature weights for the assessed organs between the feeding regimens were observed. The results indicated that in studies in which a nutritional deficiency had been applied, the weights of several body structures, most often expressed in terms of weight per 100 g BW, are strongly related to the decrease in growth rate of the fat-free body. It was concluded that most of the reported effects on growth retardation as a result of nutrient restrictions are primarily a consequence of the mobilization of fat per se.

Animal Feed↗

PubMed1997

Diphasic allometric growth of body components in white Leghorn pullets fed ad libitum and restricted diets.

Growth patterns of crude fat (cfat; ether extract), CP, ash, and water in White Leghorn pullets that ate ad libitum and restricted (feed or lysine) diets were analyzed by mono- and diphasic allometric functions, with plucked empty body mass (EBM) or fat-free EBM (FFEBM) as the independent variates. In general, the diphasic model provided a better fit to the data than the monophasic model, indicating that the allometric slope (beta) changes at a certain level of development. Data demonstrate that, under an ad libitum feeding regimen, cfat growth is proportionally (beta = 1) related to growth of the fat-free body, up to about 500 g FFEBM (pullets aged 8.5 wk). From 500 g FFEBM onwards, the beta changed to about 2, illustrating the late maturation of cfat compared to FFEBM. For feed-restricted groups, slopes and transitions for cfat growth varied from those of the control group, according to changes in dietary supply. Diphasic relationships between each fat-free body component and EBM in each treatment had different slopes for first and second phases (about 1 and 0.70). Differences, however, were mainly due to the diphasic growth pattern of cfat per se, and diminished largely if FFEBM instead of EBM was chosen as the independent variable (about 1 and 0.95). Incorrect statements on body component growth in restricted birds, due to the "masking" role of varying fat deposition rates, can be avoided if relative growth was expressed as a function of the fat-free body instead of BW or EBM. Diphasic growth relationships among the fat-free body components were not affected by feeding regimen, but by age. The beta 1 were about unity for the relationship between each fat-free component and FFEBM, suggesting a constant composition of the fat-free body in the first phase. In the second phase, CP accrued relatively faster than ash and water (beta 2 of 1.10 vs 0.94 and 0.91). Results indicate that the changes in growth pattern of fatty and fat-free tissue, both relative to the fat-free body, had not been revealed if a monophasic allometric model instead of a diphasic had been used.

Aging↗

PubMed1995

Onset of lay related to multiphasic growth and body composition in White Leghorn pullets provided ad libitum and restricted diets.

Growth of the body as a whole and of parts of the reproductive tract of White Leghorn pullets that ate ad libitum and restricted diets were analyzed by a multiphasic growth function. Parameter estimates were related to onset of lay. Chemical body compositions at defined stages of pubertal growth were related to the development of the reproductive organs. Data of pullets that had been restricted in one of two rearing periods (0 to 6 and 7 to 18 wk of age) were used. In each period, pullets were fed a low-lysine diet or a daily restricted amount of feed, on a pair-gain basis. In all mathematical fits, a well distinguishable "pubertal body growth spurt" at around 19 wk of age was found. About 40 to 70% of total growth within this phase consisted of growth of the reproductive tract (the ovary covered 19 to 35%, the oviduct 15 to 23%, and the uterus 7 to 11% of total growth). Age at maximum gain of the pubertal body growth spurt was 20.1 wk for lysine-restricted pullets and 19.6 wk for feed-restricted pullets. This difference reflected differences in onset of lay (50% rate of lay) between lysine- and feed-restricted birds (22.3 and 21.6 wk, respectively). An interval of 14 to 15 d existed between maximum gain of the pubertal body growth spurt and onset of lay. It was suggested that the occurrence of the pubertal body growth spurt can serve as a predictor for subsequent onset of lay. Body composition at defined moments during the pubertal body growth spurt was calculated by interpolation from chemical analyses of Weeks 15, 18, 20, 22, and 24. Crude fat content in the body at start of the pubertal body growth spurt varied considerably between the feeding regimens (112 to 179 g). The fat-free body (consisting of CP, ash, and water) showed less variation between the treatments at this stage (807 to 870 g). It is concluded that a particular amount of fat-free tissue is critical in pullet development and may be required before sexual organ development starts.

Age Factors↗

PubMed1993

Multiphasic analysis of growth of the body and its chemical components in White Leghorn pullets.

Data of White Leghorn pullets consuming feed ad libitum were used to study multiphasic growth during rearing and early lay. Two mathematical functions were used. Body weight gain and growth of the chemical components DM, CP, crude fat (Cfat), and ash (Ash) were described as a function of age, using a multiphasic growth function. To describe relative growth between CP and DM, Cfat and DM, and Ash and DM, a multiphasic allometric function was applied. Body weight gain from hatching to 24 wk of age was estimated most accurately by a tetraphasic growth function. The first two growth spurts represented 82% of mature BW. Growth in these two phases seemed to be related to the development of bones, muscles, and essential metabolic organs. Body growth was further characterized by a third phase at 19 wk of age, which was related to growth of the reproductive tract. The fourth phase consisted mainly of body fat deposition. Both multiphasic functions showed that the nature of component growth was diphasic, with a transition area between the two phases at around 11 wk of age. On the basis of the duration of growth and of allometric growth coefficients, growth of CP, Cfat, and Ash in the first phase seemed to be functionally related to each other and consisted of muscle growth, intermuscular fat deposition, and skeletal growth, respectively. After 11 wk of age, protein deposition is presumably a function of sexual maturity, i.e., the development of the reproductive tract; whereas fat growth is mainly abdominal fat deposition. The observed relationships indicate that the use of multiphasic functions might be helpful in the interpretation of changes in body growth and development of pullets.

Animals↗