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Biomedical subjects

B J Purswell

Publications and source records attributed to B J Purswell.

9 recordsLinked to original sources
PubMed1993

Response to gonadotrophin-releasing hormone by the intact male dog: serum testosterone, luteinizing hormone and follicle-stimulating hormone.

In Expt 1, gonadotropin-releasing hormone (GnRH) (Cystorelin, CEVA) was administered intramuscularly to two intact male dogs; one dog received one injection of 50 micrograms GnRH and one dog received four daily injections of 50 micrograms GnRH. Both dogs exhibited a significant and immediate rise in luteinizing hormone (LH) following GnRH administration, with a peak observed at 15 min following injection. Testosterone was increased over baseline concentrations for 5 and 7 days, respectively, after the injection. In Expt 2, eight intact male dogs were injected intramuscularly with 0.7 microgram GnRH (Factrel, Fort Dodge) kg-1. Baseline testosterone concentrations were established by daily sampling for 14 days before treatment. All dogs exhibited an LH peak 15 min and a testosterone peak 60 min after the GnRH injection. Testosterone concentrations had returned to baseline concentrations by 4 h after the injection. Testosterone tended to fall below baseline concentrations for several days following the injection of GnRH. No peak was noted for follicle-stimulating hormone. In Expt 3, five additional dogs were injected with 0.7 microgram GnRH (Factrel, Fort Dodge) kg-1. Testosterone concentrations rose in all dogs 1 h after the injection and returned to baseline concentrations by 24 h after injection. In the male dog, GnRH stimulated an LH peak 15 min and a testosterone peak 1 h after injection. Further investigations are needed to elucidate the different effects on testosterone concentration observed with two different GnRH preparations.

Animals↗
PubMed1991

Management of apparent luteal insufficiency in a bitch.

Serum progesterone concentration in a pregnant bitch with suspected luteal insufficiency was monitored by use of a commercially available ELISA kit, and exogenous progesterone was administered as needed to enable the bitch to deliver normal-term pups. Progesterone in oil was administered at a dosage of 2 mg/kg of body weight at 72-hour intervals. The bitch delivered 2 clinically normal male pups and 1 mummified female pup within 12 hours after the exogenous progesterone was calculated to have been metabolized.

Abortion, Veterinary↗
PubMed1989

Lymphocyte reactivity to mitogens and natural killer cell activity in crossbred swine during the reproductive cycle.

The effect of pregnancy and lactation on the cell-mediated immune response of first litter gilts was assessed using the response of circulating lymphocytes to in vitro mitogen stimulation and the cytotoxic activity of the circulating natural killer (NK) cells. Groups of gilts were sampled during the first, second and third trimester of gestation, weekly during lactation, at weaning and estrus following weaning of the piglets. No significant differences were found in the response of the cells from any of the groups to phytohemagglutinin A or concanavalin A stimulation. The natural killer cell activity, measured as cytotoxicity, decreased during gestation reaching a low point during the second and third week of lactation.

Animals↗
PubMed1988

Effect of levamisole on immune function and reproductive performance in first-litter gilts.

First-litter commercial cross-bred gilts were treated with levamisole (1.5, 2.5, or 3.5 mg/kg of body weight) weekly during the last 4 weeks of gestation, because similar treatment of dairy heifers had improved postpartum maternal health and neonatal survival. In the gilts, differences in reproductive performance were not found on the basis of pig survival at birth, pig survival at weaning, birth weight, or weaning weight. Also, differences between treated and control gilts were not found in response of circulating lymphocytes to mitogen stimulation (phytohemagglutinin A, concanavalin A, and pokeweed mitogen). In all gilts, the lymphocyte response to mitogen stimulation was decreased during the first week after farrowing.

Animals↗
PubMed1986

Effects of cyclosporin A administration in cats.

Cyclosporin A was administered orally to 10 cats for 28 consecutive days at a dosage of 20 mg/kg body weight daily divided into 2 equal doses. Serum trough CyA concentrations ranged from 134 to 902 ng/ml with a mean of 567 +/- 249 ng/ml (means +/- SD). Immunosuppression by CyA was suggested in 5 cats by significantly depressed lymphoblast transformation responses. Various hematologic, serum chemical, and urinalysis parameters were monitored on a weekly basis in 10 cats. Serum urea nitrogen concentration increased significantly from baseline values on days 7, 14, and 21, but not on day 28. Urine concentrating ability was unimpaired. Alanine aminotransferase activity was decreased significantly from baseline values at each sample period during CyA administration. Drug-related side effects were minor; one cat developed gingival hypertrophy which regressed within 21 days of CyA withdrawal.

Alanine Transaminase↗