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Biomedical subjects

B J Rosenberg

Publications and source records attributed to B J Rosenberg.

11 recordsLinked to original sources

The work environment impact assessment: a methodologic framework for evaluating health-based interventions.

BACKGROUND: A new conceptual framework is needed to evaluate health-based interventions based on the premise that like the environment, workplaces are complex ecologies. The proposed Work Environment Impact Assessment (WEIA) is analogous to Environmental Impact Assessment (EIA), a concept and method developed 30 years ago in the environmental policy arena to evaluate potential consequences of human activity for the natural environment. WEIA entails identifying and evaluating both intended and unintended consequences, or outcomes, associated with a particular intervention. Because the workplace is a complex ecology, changes in one aspect may lead to changes in other aspects. WEIA calls for a systematic and comprehensive approach to the total work environment. METHODS: To illustrate the utility of WEIA we use this approach to evaluate an intervention to reduce the public's exposure to the pesticide Alar, which had been used on apples until it was withdrawn from the market in 1989. RESULTS: While this intervention did indeed reduce the public's exposure to Alar, it also led to other unintended consequences, namely new ergonomic hazards for apple pickers, increased stress levels in the orchards for both pickers and growers, as well as new worker, and perhaps public, exposure to potent neurotoxins. CONCLUSIONS: The goal of using WEIA is not to engage in a risk-risk debate that stalls worthwhile interventions. Rather, we propose that by conducting a Work Environment Impact Assessment, all possible positive and negative "ripple" effects stemming from an intervention can be considered, so that the intervention can be designed to achieve maximum benefit.

Adult↗

A bittersweet report from a New Orleans sugar refinery: working conditions and labor-management relations deteriorate together.

At the invitation of the president of United Food and Commercial Workers (UFCW) Local #1101, which represents workers at the Domino sugar refinery in Arabi, Louisiana, we met with several union members in the fall of 1998. Our purpose was to investigate workers perceptions of health and safety conditions in the plant and offer assistance in the amelioration of problems. We conducted in-person interviews, each lasting approximately 30 minutes, with the local union president and twelve members selected by the president.

Journal Article↗

A radioreceptor assay in which iodocyanopindolol is used to determine propranolol and its active metabolites in unextracted serum or plasma.

This rapid, sensitive, simple radioreceptor assay (RRA) for l-propranolol and its active metabolites in unextracted samples requires 5 microL of sample, a beta-adrenergic antagonist, 125-l-labeled (-)cyanopindolol (125ICYP), and turkey erythrocyte membrane receptors (Kd = 40 pmol/L). Equal volumes (100 microL) of diluted sample and 125ICYP are incubated with 500 microL of erythrocyte membranes for 30 min. Cold isotonic saline (2.5 mL) is added, and the mixture is centrifuged. The concentration of drug that inhibited receptor binding by 50% (IC50) was l-propranolol, 1.5 micrograms/L; d-propranolol, 243 micrograms/L; and 4-hydroxypropranolol, 8.8 micrograms/L. Analytical recovery of propranolol added to serum was 93 to 120%; results for 138 clinical samples tested by this method and by liquid chromatography correlated well (r = 0.96). Results correlated strongly (r = 0.98) for 23 clinical samples tested by RRA and analyzed for both propranolol and 4-hydroxypropranolol by liquid chromatography. The sensitivity of this RRA was 0.3 micrograms/L, and both intra- and interassay CVs were less than 12%. Modifications of this method could permit testing of other nonselective beta-blockers.

Adrenergic beta-Antagonists↗

Plasma levels of thiothixene by radioreceptor assay: clinical usefulness.

Thirty-four newly admitted schizophrenic patients were treated with a fixed dose of thiothixene (0.44 mg/kg) for 4 weeks. Thiothixene and its active metabolites were measured by a new radioreceptor assay. Improvement occurred over the entire range of recorded plasma levels, but the chances of substantial improvement appear greater above 40 neuroleptic units (n.u.). The data do not support the notion of a "therapeutic window", in that higher plasma levels were not associated with side effects or clinical deterioration (although at extreme plasma levels this must of course be so). In 11 nonresponders dosage could not be increased because of side effects. If a non-responder with troublesome side effects has a low plasma level (less than 40 n.u.), it would seem prudent to switch to another antipsychotic drug.

Adult↗

Influence of blood sampling methods on dopamine-receptor-blocking activities as determined by a radioreceptor assay.

The influence of blood collection methods on dopamine-receptor-blocking activities as determined by a radioreceptor assay kit was investigated. Thirty-one patients treated with one of six neuroleptic drugs (thioridazine, trifluoperazine, haloperidol, chlorpromazine, thiothixene, or fluphenazine) participated in this study. Blood samples were drawn from each patient into five different evacuated blood collection tubes made by the same manufacturer (red-stoppered tube containing no additives, lavender-stoppered tube containing EDTA, green-stoppered tube containing heparin, dark blue-stoppered tube containing no additives, and dark blue-stoppered tube containing heparin). The results show that for five drugs (chlorpromazine, fluphenazine, haloperidol, thiothixene, and trifluoperazine), the dark blue-stoppered tubes without additives resulted in significantly higher dopamine-receptor-blocking activities than the red-, lavender-, or green-stoppered tubes. For thioridazine, the green-stoppered tubes resulted in significantly higher blocking activities than the blue- and red-stoppered tubes. The possible effect of tris(2-butoxyethyl) phosphate, a plasticizer, on dopamine-receptor-blocking activities by neuroleptic drugs is discussed.

Antipsychotic Agents↗

Comparative effects of a series of prolactin inhibitors, 17beta-estradiol and 2alpha-methyldihydrotestosterone propionate, on growth of 7,12-dimethylbenz(a)anthracene-induced rat mammary carcinomas.

Eight ergot alkaloids and ergoline derivatives, effective prolactin inhibitors, were tested for activity against DMBA-induced rat mammary carcinomas. Compounds were administered daily, 5 times/week for 4 weeks, and rats were observed for an additional 4 weeks. Groups treated with androgen and estrogen were used as positive controls. Those ergot compounds and ergolines that proved to be highly effective in reducing tumor size or in inducing regression of tumors to nonpalpability were Deprenon (D-6-methyl-8-ergolin-I-ylacetic acid amide) and ergocryptine; effective to an intermediate degree were Dironyl [N-(D-6-methyl-8-isoergolin-I-yl)-N',N'-diethylurea], ergocornine, and Lysenyl [N-(D-6-methyl-8-isoergolenyl)-N',N'-diethyl-urea]; and effective to a minimal degree were Lergotrile (2-chloro-6-methylergoline-8beta-acetonitrile), CB-154, and 6605-VUFB (D-6-methyl-8-cyanomethylergolin-I). Remission of many individual carcinomas was brief, and duration of complete regression (all tumors in the rat were nonpalpable) was less than 10 weeks.

9,10-Dimethyl-1,2-benzanthracene↗

Direct analysis of dehydroisoandrosterone in plasma.

DHA (1) has been measured in plasma by a radioimmunoassay procedure using an antiserum to DHA-7-BSA whose specificity is such that the procedure is carried out directly on diluted, unextracted plasma. The method has been used to obtain plasma DHA secretory patterns and mean concentrations and the data are in accord with those determined by related but more laborious techniques.

Adrenalectomy↗

24-Hour secretory pattern of dehydroisoandrosterone and dehydroisoandrosterone sulfate.

Dehydroisoandrosterone (DHA) and cortisol were measured by radioimmunoassay and protein binding techniques respectively in plasma from blood taken at 20-min intervals over 24-h periods in 3 normal men, 2 women with Stein-Leventhal syndrome and a man with a benign adrenocortical adenoma. In all subjects but the latter, DHA and cortisol were episodic and synchronous throughout the entire day; in this patient, continuous secretion of cortisol by the tumor apparently abolished stimulation of the contralateral adrenal, and DHA production was negligible. Dehydroisoandrosterone sulfate analysis in plasma displayed a pattern which, probably because of its origin both by secretion and sulfation and its long half-life showed less synchronicity with DHA and cortisol and less fluctuation than did the free hormones.

Adenoma↗