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B J Zeligs

Publications and source records attributed to B J Zeligs.

7 recordsLinked to original sources
PubMed1992

Poly I:C accelerates development of diabetes mellitus in diabetes-prone BB rat.

We developed a new experimental model of accelerated diabetes mellitus in the genetically susceptible diabetes-prone BB rat with the administration of the IFN-alpha inducer poly I:C. With this model, there was both an increased incidence and accelerated onset of insulin-dependent-diabetes in poly I:C-treated animals compared with saline-treated controls. All twelve rats administered poly I:C (5 micrograms/gm body weight 3 times/wk) developed diabetes by 57 days of age (100%) compared with 1 of 27 (3.7%) saline-treated controls. Furthermore, the development of diabetes was accelerated in the poly I:C-treated group (mean age +/- SE at onset 52.8 +/- 0.58 days) compared with saline-treated controls (89.3 +/- 2.4 days, P less than 0.01). Additionally, poly I:C-treated rats had higher mean serum IFN-alpha levels than saline-treated rats at weeks 2 and 3 of treatment (210 vs. 27 and 183 vs. 25 U/ml, respectively, P less than 0.001). Poly I:C treatment of 5 Wistar rats, the parental strain, which is not susceptible to diabetes, did not result in insulitis, diabetes, or hyperglycemia. The histopathologic findings of insulitis and decreased immunoreactive islet insulin in poly I:C-accelerated diabetic BB rats and in BB rats with spontaneous diabetes suggest a similar pathophysiology.

Analysis of Variance↗
PubMed1978

Ideal target organism for quantitative bactericidal assays.

We have developed a target organism which permits quantitative bactericidal assays. The organism is an Escherichia coli mutant which cannot grow at the temperature of the assay (37 degrees C), but retains full colony-forming potential for subsequent quantitation at 25 degrees C. We show that quantitative data on the bactericidal capacity of polymorphonuclear leukocytes and alveolar macrophages can be obtained when this mutant is used as a target. The procedure used to generate the strain is described in detail and should be applicable to many bacterial species. Characterization of the properties of the mutant indicates that it has a strong potential for use in other in vivo and in vitro investigations of host responses to microbial invasion.

Animals↗
PubMed1977

Maturation of the rabbit alveolar macrophage during animal development. I. Perinatal influx into alveoli and ultrastructural differentiation.

Rabbit alveolar macrophages (AM's) were collected by tracheobronchial lavege at sequential times during animal development. The total number of cells recovered by this technique was found to increase markedly shortly before birth (Fig. 4). This apparent influx of macrophages into the alveoli continued during the first postnatal week, and, at a reduced rate, throughout the first postnatal month of life (Fig. 3). Ultrastructurally, AM's of the prenatal period resembled their monocyte precursors, and contained modest numbers of lysosomes and mitochondria, scant lamellae of ribosome-studded endoplasmic reticulum (RER), and a small Golgi apparatus (Fig. 12). A considerable amount of phagocytosed material was observed in these AM's, and consisted largely of cellular debris and two forms of surfactant-related phospholipids, termed tubular and lamellar myelin (Figs. 12-15). The quantity of these ingested phospholipids increased dramatically shortly after birth, in correlation with the known release of similar material from type II pneumocytes at this time. (Figs. 16 and 19). During the first postnatal week AM's showed a considerable increase in number of mitochondria and in the development of the RER and Golgi apparatus (Fig. 22). Increased accumulations of lipid droplets were also noted during this period. Ingested material continued to consist largely of surfactant-related phospholipids, but was less abundant at this time. By 28 days after birth, AM's were nearly morphologically mature (Fig. 25). They showed large numbers of lysosomes and mitochondria, and well developed RER and Golgi apparatuses. Ingested phospholipid material was still visualized, and the incomplete degradation of this material appeared to give rise to the dense intraphagolysosomal whorls characteristic of the mature AM.

Animals↗
PubMed1977

Maturation of the rabbit alveolar macrophage during animal development. III. Phagocytic and bactericidal functions.

Phagocytic and bactericidal function of rabbit alveolar macrophages (AMs) lavaged from animals during the course of postnatal maturation was studied. Staphylococcus aureus and a temperature-sensitive mutant of Escherichia coli, which could not replicate at 37 degrees during the functional assays, were employed as test bacteria. Assays of the phagocytic capacity of AMs from rabbits of various age groups revealed no significant differences either in the percentage of AMs which took up bacteria (79-90%) or in the number of bacteria taken up per AM (Table 1). In contrast, bactericidal activity of AMs was found to increase with increasing animal age. No bactericidal activity was detected in AMs from newborn animals (Figs. 1 and 2), whereas AMs from 7-day-old animals exhibited at least a bacteristatic activity against S. aureus (Fig. 1) and AMs from 28-day-old rabbits showed marked bactericidal activity, essentially the same as that of AMs from adult rabbits. Adult AMs killed 75% of the S. aureus and 60% of the E. coli within 120 min (Figs. 1 and 2).

Aging↗