[Opsonizing effect of the 19S and 7S bovine serum globulins].
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Age Factors↗
Biomedical subjects
B Jacob
Publications and source records attributed to B Jacob.
Effect of stabilising amino acids on the digestive absorption of heme and non-heme iron.
We used the Ussing chamber model to study heme iron absorption by rat duodenal mucosa. Heme iron was obtained by enzymic digestion of bovine haemoglobin and concentration of heme (HPH). Its uptake and mucosal transfer was compared to iron gluconate (Gluc), at 100 microM and 1 mM. At 100 microM iron uptake (Qtot), mucosal retention (Qm) and transfer across the mucosa (Qs) was similar for the two sources of iron. Qs was significantly higher at 1 mM for Gluc but not for HPH, and was associated with higher levels of Qm. Addition of L-histidine did not improve iron absorption and indeed it decreased it if iron was provided as Gluc. L-cysteine increased the transfer of iron of both sources. In the in vitro model using rat digestive mucosa, heme iron appeared to be an efficiently used source of iron, which might prevent its accumulation by gut when supplied in excess.
Amino Acids↗
The use of SPECT in a multidisciplinary memory clinic.
We tested the interobserver reliability of visual rating of HMPAO-SPECT imaging in 271 outpatients referred to a memory clinic, and followed over 1 year. The clinical diagnoses were Alzheimer's disease (n = 156), frontotemporal dementia (n = 47); vascular dementia (n = 21), senile dementia of Lewy body type (n = 12), anxiety/depressive disorders (n = 14) and miscellaneous memory disorders (n = 21). The interobserver agreement was good (k = 0.68). However, the heterogeneity of the patterns-independent from demographic data, age at onset and duration of the disease- and their lack of sensibility and specificity limited the contribution of SPECT for diagnostic purposes in routine practice.
Aged↗
[Ultrastructural differences of fibrin coagulation before and after cardiovascular arrest].
The reconstruction of killing offences often requires the differentiation between vital and postmortal blood traces at the sites of action and finding. Earlier investigations seemed to imply the possibility of such a differentiation depending on the different clot structure. We therefore examined clots prior and after 10 minutes of complete occlusion of the upper extremity morphologically and morphometrically with the REM. The morphologic comparison of these clots revealed narrow, round shaped fibrin fiber networks for clots of native blood, whereas clots after occlusion showed linear, rarely connected long fibrin fibers. The morphometry of the fibrin free spaces revealed a significant enlargement of the single fibrin free spaces as well as of the total fibrin free space after occlusion. For capillary blood a significant decrease in fiber diameter was observed after occlusion. The examination of clinical clotting parameters revealed alterations of thrombocyte aggregation for the collagen induction, a slightly reduced thrombocyte retention and slightly steeper flanks in the thromboelastogram after occlusion. The reason for these changes was interpreted as lack of high energetic phosphates in the retraction process as the clotting cascade itself requires a very high amount of these phosphates. Use of these results in the forensic practice will have to take factors as temperature, drying out, surface-structure and many others into account.
Blood Coagulation↗
Frequency and topographical distribution of CD68-positive macrophages and HIV-1 core proteins in HIV-associated brain lesions.
We report the neuropathological and immunohistochemical findings in the brains of 14 AIDS patients with HIV-related encephalopathy. Clinically, half of the patients presented with severe AIDS dementia complex including advanced psychomotor retardation and behavioural dysfunction. These features correlated with striking cerebral atrophy and subcortical lesions visible in CT and/or MRI scans. In 7 cases early signs of impaired memory and concentration and/or psychomotor slowing were apparent accompanied by subcortical lesions in MRI scans and normal CCTs. In order to investigate the topographical distribution of HIV-1-associated features, in every case tissue samples from the frontal, temporal, parietal, occipital cortex and subcortical white matter, the hippocampus, basal ganglia, midbrain, pons, medulla oblongata and cerebellum were studied. In all patients histological examination disclosed the typical cellular constituents of HIV encephalitis (n = 12) or leukoencephalopathy (n = 2). Antibodies against lymphocyte subsets, CD68 antigen, myelin basic protein and GFAP were used to characterize the phenotype of cells and to highlight the white matter gliosis. The distribution and degree of pathological features were analysed in a semiquantitative scale, based on the number of CD68-positive cells, and disclosed great interindividual differences concerning the affected brain regions which only in part correlated with the severity of the clinical picture. It is noteworthy, that the deep gray matter, in particular putamen and thalamus, was involved in every case, independent from the stage of the disease. In addition, quantity and topographical distribution of HIV-1 core protein p24 were studied by use of two monoclonal antibodies. It is noteworthy, that the number of immunoreactive multinucleated giant cells and microglial cells decreased gradually from the deep gray matter, especially putamen and thalamus, and deep white matter to corpus callosum, cerebellar white matter and subcortical cerebral white matter. The topographical predilection of the deep gray matter even in cases with early cognitive decline indicates that the basal ganglia are affected early in the course of the disease. This observation closely resembles the results of highly sensitive quantitative neuropsychological tests which disclosed slowing and impaired coordination of rapid extremity movements indicating basal ganglia lesions even in early stages of HIV dementia.
AIDS Dementia Complex↗