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Biomedical subjects

B Jiang

Publications and source records attributed to B Jiang.

216 records · Page 12Linked to original sources
PubMed1991

Nonlinear algorithm for identification of a fiducial marker for various cardiac events.

We report on a nonlinear algorithm which identifies R-wave peaks on the surface electrocardiogram, consistent reference points on the left ventricular pressure waveform and the initiation of the QRS complex on the epicardial electrogram. The algorithm has been used to evaluate data from horses, ponies, dogs and humans at rest and during exercise. It permits rapid, accurate evaluation of data on a beat-by-beat basis even with noisy signals and varying waveform configurations. The algorithm facilitates the acquisition of detailed information previously difficult or impossible to obtain by more conventional methods of analysis.

Algorithms↗
PubMed1991

The therapeutic and biological effects of total lymph node irradiation and autologous bone marrow infusion on malignant lymphoma patients.

Forty patients with malignant lymphoma were treated by 60Co total lymphoid irradiation (TLI): 21 cases received 6 Gy and 19 received 8 Gy. Ten also received autologous bone marrow infusion (ABMI). Acute radiation damage with digestive tract reaction and hemopoietic and immunological depression was observed. Bone marrow was depressed. WBC and platelets decreased rapidly. Lymphocytes showed quantitative and qualitative changes even at the early stage. All these symptoms subsided within 40 days. TLI accompanied by irradiation of the tumor site could result in effective control. The 1-, 3-, and 5-year survival rates of malignant lymphoma patients were 30/40 (75%), 14/24 (58%) and 4/12 (33%), respectively, while in those with Hodgkin's disease alone, the 1- and 3-year survival rates were 10/13 (76%) and 5/7 (71.4%), respectively. ABMI hastened hemopoietic reconstitution, which recovered relatively quickly after TLI.

Adolescent↗
PubMed1991

Digital signal analysis of cardiac events in horses and ponies.

We have developed a digital signal analysis technique which can be used to evaluate various cardiac events in ponies and horses at rest and during exercise. The algorithm is designed to identify R-wave peaks on the surface electrocardiogram, consistent reference points on the left ventricular pressure waveform and the initiation of the QRS complex on the epicardial electrogram. We have used the technique to evaluate data from 10 horses and ponies at rest, during strenuous exercise and during experimentally-induced coronary artery occlusion. The technique provided rapid and accurate beat-by-beat evaluation of data, even with relatively noisy signals and under conditions in which the waveform configurations were not consistent. The benefits afforded by this technique make it especially useful for extracting information which previously was difficult or impossible to obtain using more conventional methods of analysis.

Algorithms↗
PubMed1991

[Analysis of main transmission factors of hepatitis A].

A case control study (1:2) of 182 pairs of Hepatitis A was carried out in Weng-ling Zhe-jiang during April 1988. The results showed that adjusted odds ratio (aOR) had a dose-response relationship with exposed different food factors.

Case-Control Studies↗
PubMed1990

Structural requirements of ATP for activation of basal and atrial natriuretic factor-stimulated guanylate cyclase in rat lung membranes.

ATP has been reported to increase basal and atrial natriuretic factor (ANF)-stimulated guanylate cyclase activity. The structural features of ATP involved in the activation of guanylate cyclase were examined by employing a variety of ATP analogs with modification either at the phosphate chain or at the ribose moiety. Among the natural adenine nucleotides, ATP and ADP were able to increase both basal and ANF-stimulated guanylate cyclase activities in rat lung membranes. AMP had no effect. ATP was more effective than AMPPCP (the non-hydrolyzable analog of ATP), and ADP was more effective than ADP beta S and AMPCP (the hydrolysis-resistant analogs of ADP) to increase basal and ANF-stimulated guanylate cyclase activities. Removal of the oxygen atom from the ribose moiety of ATP or ADP significantly reduced their potency. Thus, the length of the phosphate chain and the hydroxyl groups at the ribose moiety are both determinants for nucleotide mediated guanylate cyclase activation.

Adenosine Diphosphate↗
PubMed1990

Caged ATP potentiates guanylate cyclase activity stimulated by atrial natriuretic factor in rat lung membranes.

ATP increases both basal and atrial natriuretic factor (ANF)-stimulated guanylate cyclase activities. The present studies were designed to compare the effect of adenosine-5'-triphosphate, P3-1-(2-nitrophenyl)-ethyl ester (caged ATP), a photolabile derivative of ATP, with ATP. Caged ATP increases both basal and ANF-stimulated enzyme activities in a concentration-dependent manner, but is more potent than ATP. The effect of caged ATP significantly decreases after it is photo-converted to ATP by UV irradiation. These observations suggest that the caged group introduces a hydrophobic interaction thereby facilitating guanylate cyclase activation.

Adenosine Triphosphate↗
PubMed1990

Characterization of ATP-stimulated guanylate cyclase activation in rat lung membranes.

Many of the effects of ANP are mediated through the elevation of cellular cGMP levels by the activation of particulate guanylate cyclase. While the stimulation of this enzyme is receptor-mediated, the molecular mechanism of activation remains unknown. In this study we present evidence that ATP as well as its analogues adenosine-5'-O-(3-thiotriphosphate) (ATP gamma S) and adenylylimidophosphate (AMPPNP) activates guanylate cyclase from rat lung membranes and markedly potentiates the effect of ANP on the enzyme. The order of potency is ATP gamma S greater than ATP greater than AMPPNP. The enzyme activation by adenine nucleotide and ANP together is much more than the sum of the individual activations, suggesting that ATP may be the physiological component essential for the ANP-stimulated guanylate cyclase activation. The ATP gamma S-stimulated guanylate cyclase activity diminishes in the presence of various kinds of detergents, suggesting either that the conformation of an ATP binding site in guanylate cyclase is altered by detergents or that protein-protein interaction may be involved in the activation of guanylate cyclase by ATP. Guanylate cyclase from rat lung membranes is poorly activated by ANP and/or ATP gamma S after removing the cytosolic and weakly membrane-associated proteins or factors by centrifugation. Pre-incubation of the membranes with ATP gamma S retains enzyme activation after membrane washing. These results suggest either that ATP gamma S stabilizes the conformation of nucleotide binding site in guanylate cyclase from denaturation by membrane washing, or that the stimulatory effect of ATP on guanylate cyclase activity may be mediated by accessory proteins or non-protein cofactors which are lost during membrane washing, but remain bound to membranes by ATP gamma S pretreatment.

Adenosine Triphosphate↗
PubMed1990

Expression of a fast fiber enzyme profile in the cat soleus after spinalization.

This study was designed to determine the effects of reduced neuromuscular activity on the expression of proteins associated with contractile and metabolic functions and the size of single muscle fibers in the cat soleus. Adult cats were spinalized (Sp) at T12-T13 and maintained in a healthy condition for 6 months. Some of the cats were trained to weight-support (Sp-WS) for 30 minutes per day beginning one month posttransection. Cross-sectional area (CSA), succinate dehydrogenase (SDH), alpha-glycerophosphate dehydrogenase (GPD), and myofibrillar adenosine triphosphatase (ATPase) activities were determined in a population of single fibers identified in frozen serial cross-sections. Each fiber was categorized as either light or dark based on its staining density for qualitative myosin ATPase, alkaline preincubation (pH 8.75). The Sp (45%) and Sp-WS (31%) groups had significantly higher percentages of dark ATPase fibers than control (less than 1%). All dark ATPase fibers were shown to react positively for a fast myosin heavy chain monoclonal antibody, while some of these fibers showed a reaction to both fast and slow myosin heavy chain antibodies. Overall mean fiber CSA were significantly smaller (approximately 25%) than control in both Sp groups. In the Sp-WS, but not the Sp cats, the dark fibers were larger than the light fibers (P less than 0.05), suggesting a preferential effect of postural training on the ATPase converted fibers. There were no significant differences among the three groups in any of the mean enzyme activities for either ATPase type fiber. However, there was a general tendency for the Sp cats to have elevated GPD and ATP activities per muscle; this appeared to be directly related to the percentage of fibers staining darkly for myosin ATPase. These data indicate that 6 months after spinalization some of the fibers of the slow muscle developed fast myosin staining patterns and oxidative and glycolytic enzyme profiles that are normally exhibited in fast fatigue-resistant motor units. Periods of daily weight-support appear to ameliorate some of these adaptations to spinalization. Further, the observation that SDH activities are maintained at control values in spinalized adult cats as well as in spinalized kittens (unpublished observations) suggest that, at least in the soleus, skeletal muscle fibers can maintain their oxidative potential even though there is a marked reduction in neuromuscular activity for 6 months.

Adenosine Triphosphatases↗
PubMed1990

Enzymatic plasticity of medial gastrocnemius fibers in the adult chronic spinal cat.

The metabolic plasticity of single fibers in adult cat medial gastrocnemius (MG) 6 mo after complete spinal cord transection (Sp) at T12-T13 was studied. Some Sp cats were trained to weight support (Sp-WS) 30 min/day beginning 1 mo posttransection. Cross-sectional area, succinate dehydrogenase (SDH), alpha-glycerophosphate dehydrogenase (GPD), and myofibrillar adenosinetriphosphatase (ATPase) activities were determined in fibers identified in frozen serial sections. Fibers were categorized as light or dark based on myosin ATPase staining, alkaline preincubation. The percentage of dark ATPase fibers was higher in Sp and Sp-WS (approximately 85%) than in control (approximately 60%). All dark ATPase fibers reacted positively to a fast myosin heavy chain monoclonal antibody. In both spinal groups, a higher percentage of dark ATPase fibers reacted to both fast and slow myosin heavy chain antibodies than in controls. Neither Sp nor Sp-WS cats showed fiber atrophy. Compared with control, SDH activity was decreased in both fiber types of Sp cats. Daily weight-support training ameliorated this adaptation. There were no differences among the three groups in mean GPD and ATPase activities for either fiber type. There was a slight tendency, however, for spinal cats to have higher GPD and ATPase activities (independent of type) than control, probably reflecting the larger proportion of dark ATPase fibers in these cats. These observations indicate that 6 mo after spinalization in adult cats, some of the fibers of a fast muscle became "faster" and developed oxidative and glycolytic enzyme profiles that normally are exhibited in fast fatigable motor units.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenosine Triphosphatases↗
PubMed1990

[Studies and comparisons on chemical components of essential oils from Clematis hexapetala Pall. and Inula nervosa Wall].

Chemical components of the essential oils from clematis hexapetala and Inula nervosa were analyzed by using GC-MS-DS. The result shows that the major components of the essential oil from Inula nervosa are thymol and thymol isobutyrate, while the major components of the essential oil from Clematis hexapetala are palmitic acid and 3-hydroxy-4-methoxyl benzaldehyde.

Drugs, Chinese Herbal↗
PubMed1990

Allogeneic bone marrow transplantation for the treatment of leukemia.

Eighteen patients with leukemia have received HLA-identical allogeneic bone marrow transplantation (BMT) at our hospital since 1981. Fifteen of these patients have been living without relapse. for prophylaxis of GVHD, MTX was used in 8 patients, and cyclosporine (CSP) together with MTX in 6 patients, 3 received multiple agents at much smaller dosage, including monoclonal antibody. All patients received intravenous placental gamma-globulin, and 16 received garlic extract. Three patients died. One, who neither received MTX, nor CSP died of hyperacute GVHD, one who did not receive garlic extract died of GMV pneumonia, and the third one died of tuberculosis 18 months after BMT.

Adolescent↗
PubMed1990

[Observations of the tracheal microvasculature in the rat].

Blood vessels of the trachea and microcirculation of tracheal wall in rats were investigated using vascular casts and ink injection. The tracheal blood vessels were found to be distributed segmentally. The longitudinal tracheo-esophageal arteries sent out transverse vessels to the soft tissue between the cartilages. These transverse vessels were anastomosed in the midline of the anterior tracheal wall and continued to divide into smaller branches to supply areas covered by cartilaginous rings. The blood bed in the inner aspect of tracheal cartilage was made of very rich capillaries and dense venous sinuses. The arterial branches to the posterior membranous portion of the trachea came from esophageal and tracheal arteries.

Animals↗
PubMed1989

Structural and functional analysis of murine skeletal muscle after castration.

A previous histochemical and morphometric study demonstrated that castration caused a significant decrease in myofiber and muscle size in the extensor digitorum longus (EDL) muscles of male ReJ 129 mice. The objective of the present study was to examine the effects of castration on the morphology and physiology of the EDL. Muscle wet weight was significantly decreased in the castrated group. The morphological survey demonstrated a significant degree of degeneration in the EDL muscles from castrated animals, consisting of disruption of normal myofilament arrangement and necrosis, invasion of active macrophages, and atrophic myofibers. These findings were significantly correlated with the decrease in peak tension observed in the castrated groups. No significant difference was detected in the time to peak tension and half-relaxation time in the castrated groups compared with the control. Thus, alterations of the morphological integrity and myofiber size of skeletal muscle affect muscle strength in castrated animals.

Animals↗
PubMed1988

A system for deriving revertants of oncogene-transformed human cells.

We have previously shown that some transformed derivatives of the human osteosarcoma-derived cell line HOS are killed by treatment with 1 microM ouabain at pH 8.2, whereas their nontransformed counterparts are relatively unharmed by the same conditions. HOS cells transformed by v-Ki-ras and RAS, v-fms, or MET are susceptible to 1 microM ouabain while those transformed by v-fes are not. Here we describe the adaptation of this differentially cytotoxic effect as a method to enrich for cells which revert to a nontransformed phenotype. We have optimized parameters which increase the differential cytotoxicity, including pH and potassium concentration during and subsequent to ouabain treatment. The efficiency of this procedure was tested in mixed cell experiments where model populations were constructed consisting of HOS cells mixed with an excess of v-Ki-ras-transformed HOS cells. Two successive OAK treatments (ouabain/alkaline/K+-free) were sufficient to recover nontransformed cells free of ras-transformants as indicated by genetic markers and morphology. This HOS/ouabain system is currently being used to derive revertants of ras-transformed human cells and could facilitate the isolation of genes interacting in the pathways by which these cells are transformed.

Cell Line, Transformed↗
PubMed1982

An analysis on the equivalence of the eye to a system with aberration.

Computed transfer functions for epsilon = 0.60 and 0.75 annular pupils of 2.67 mm o.d. show that absorption of the average spherical aberration of the eye is still incomplete but if the eye is so designed as to adjust its focal plane in order to achieve a best Strehl ratio, it would show a transfer function approaching that of a diffraction limited system, i.e. the optical defect of the eye is overcompensated. However, it is argued that a varying amount of focusing adjustment of the eye is needed in order to account for the transfer function of the eye of different pupil sizes, though not always to the extent of achieving a best Strehl ratio. Such a property of the eye is referred to variously as the antidefocusing, deblurring or contrast constancy phenomenon and, when operationally defined, this is equivalent to saying that the eye has a greater depth of focus than that defined for a physical system based upon a fixed demodulation criterion. The significance of the physiological depth of the eye is discussed.

Accommodation, Ocular↗
PubMedDate not supplied

Beneficial effect of hepatic stimulatory substances on the survival of intrasplenically transplanted hepatocytes.

Intrasplenic hepatocyte transplantation has been demonstrated to have a tentative role in treating experimental liver disease, but methods for promoting the rapid proliferation of intrasplenic hepatocytes are still quite limited. In this study, hepatic stimulatory substances (HSS) obtained from regenerating porcine livers were injected directly into the subcutaneously translocated spleens of recipient rats that had received intrasplenic hepatocyte transplantation. The clusters of intrasplenic hepatocytes contained more than 100 cells, and formed cord structures at 2 wk after transplantation, and the hepatocytes still survived at 6 wk in the HSS-treated rats. In contrast, the clusters contained less than 10 hepatocytes at 2 wk after transplantation, and no surviving hepatocytes was observed at 4 and 6 wk in control rats. Additionally, marked proliferation of bile ductular-like structures appeared around the clusters of surviving hepatocytes in the splenic red pulp of the HSS-treated rats, but were not found in control rats at 4 and 6 wk after transplantation.

Animals↗