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Biomedical subjects

B Joković

Publications and source records attributed to B Joković.

At least 19 recordsLinked to original sources
PubMed1996

[Epidemiology of poststreptococcal glomerulonephritis in the military population].

Acute poststreptococcal glomerulonephritis appears often in children and our interest is the incidence of the disease in young adults (aged 18 to 22 years) especially in military people. In Nephrology Clinic of the Military Medical Academy (In the period from 1978 to the end of 1993) 153 patients were treated and sporadic cases developed after respiratory infection. Only 5 patients developed the disease after skin infection with Streptococcus B hemolyticus group A. A large number of patients acquired the disease in the period from February to April and from October to December and most of them in the time interval from 5 to 7 years. A substantial, number of patients were treated in 1982 (13), followed by 22 in 1989, and the trend continued in 1990 (24 patients) while only 6 patients presented in 1993. So, the incidence of the disease in the military population is not high (10 +/- 6 patients year and it was sporadic cases and more after respiratory infection.

Adolescent↗
PubMed1993

[Modern immunologic tests in the diagnosis of Lyme borreliosis].

In this work we described the results that were obtained using various immunodiagnostic assays for the detection of lyme borreliosis. Sera of the patients that were in acute or chronical phase of the disease were analysed in indirect immunofluorescent, immunoenzyme and immunoblot assays which were prepared and carried out in our laboratory. As a control for the validity of these investigations, we used sera of the healthy people, as well as of the patients suffering of lues or rheumatoid illnesses. Results that we obtained pointed out the factors responsible for the nonspecific reactions in indirect immunofluorescent and immunoenzyme test. The advantage of the immunoblot analysis in detecting lyme borreliosis is described in this work.

Humans↗
PubMed1981

[New possibilities of diagnosing intestinal helminthiasis by flotation of ova in a honey medium].

Aqueous honey solution of 1350 specific weight, was used as a medium for flotation of intestinal helminth eggs. By the method of Lörincz 446 stools of soldiers were examined by parallel flotation in honey and glycerine. By flotation in honey solution helminth eggs were found in 168 (37,6%) and by flotation in glycerin in 96 (21,5%) which made the difference of 72 (16,1%). The most commonly were found: Trichuris trichuria eggs in honey solution in 108 (24,2%) stools and in glycerin in 86 (15,2%); Ascaris lumbricoides eggs in honey solution in 39 (8,7%) stools and, in glycerin in 10 (2,2%); while eggs of other intestinal helminths were rarely found and if they were found their number was approximately same obtained by both methods. Aqueous honey solution has shown to be a very suitable medium for flotation of intestinal helminth eggs, especially in cases when stools contain a small number of eggs and that mainly immature eggs of Ascaris lumbricoides which can be hardly found by flotation in glycerin.

Feces↗
PubMedDate not supplied

[Serotyping and pyocin typing of Pseudomonas aeruginosa in a study of intrahospital infections].

Typing of Pseudomonas aeruginosa strains is the necessary precondition for the study and control of intrahospital infection caused by this microorganism. Since O-serotype is considered to be the basic epidemiological marker we have studied presence and distribution of some O-serotypes of 235 Pseudomonas aeruginosa strains isolated from 131 patients. The following O-serotypes have been most frequently found: O11 (21%), O6 (18%) and O12 (16%). The combination of sero and pyocin typing proved the presence of epidemic strains at departments of burns and orthopedics.

Bacterial Typing Techniques↗
PubMedDate not supplied

[Typing of methicillin-resistant Staphylococcus aureus with a specific type of resistance].

Methycillin resistant Staphylococcus aureus (MRSA) is the important causative agent of intrahospital infections (IHI) which could have epidemic character. Typization of strains is necessary for control of these infections. Fifty eight strains of MRSA with specific resistance to antibacterial agents have been isolated from patients at different clinics of our hospital. The determination of resistotype, biotype and plasmid profiles of these and control strains of Staphylococcus aureus (SA) was aimed at confirmation whether identical strains were in questions. Biotypization showed no difference between the studied and control strains while determination of the plasmid profile proved the presence of an epidemic strain which caused infection in several clinics of our hospital.

Bacterial Typing Techniques↗
PubMedDate not supplied

[Reaction of the injured organism to infection].

Authors have examined degree of the immune response to experimentally inoculated agent in the early (6 hours) and late (7 days) phase of the organism response to thermal injury in BALB/c rats divided into two experimental groups and three control groups (only infected, only burnt and intact). Brucella abortus bovis of VA-19 species known as a good immune response stimulator in the host was i.p. inoculated in LD 50 (1 x 10(7) bact/ml). as the infective agent. Thermal injury-scalding was induced after the Art-hurson's method. Obtained results after observed brucella multiplication and spreading in blood, spleen, liver and lymph nodes as well as after determination of the phagocyte activation degree measured by the lumisol-dependent-chemiluminescence technique have pointed out that the general response to also confirm reliability of serologic diagnosis of toxoplasmosis by IgM antibody detecting in disease outbreaks. Acute acquired toxoplasmosis could not be reliably diagnosed on the basis of IgG-ELISA and IgG-IIF test findings. Enzyme immunoassays-ELISA are significantly more sensitive and more specific than IIF ones.

Animals↗
PubMedDate not supplied

[Use of the ELISA immunoenzyme test and indirect immunofluorescence in the diagnosis of a toxoplasmosis epidemic].

Etiologic agent of lymphadenitis outbreak in one our military environment was detected by two serologic tests: ELISA (enzyme-linked immunosorbent assay) and indirect immunofluorescence test (IIF) both specific for IgM and IgG antibody detection in toxoplasmosis diagnosis. IgM-ELISA analysis of the first serum sample taken from a group of 79 soldiers with lymphadenitis was positive in 65 examinees (82.28%) and IgG-IIF technique in 52 (65.82%). In a group of 33 soldiers from the same unit with no signs of the disease IgM-ELISA test was positive in 12 (36.36%) and IgM-IIF in 9 (27.27%). In a group of 40 officers from the unit with the disease outbreak specific IgM antibodies were found by both tests only in the head cook. IgM antibodies were not found in a control group of 102 healthy soldiers from other units of the same garrison. Results of this investigation thermal injury showed variations in immune response to infection in dependence on trauma effect duration (in dependence on the phase). In the early posttraumatic phase organism is capable to react effectively to the inoculated infective agent and in the late posttraumatic phase this reaction is much less effective. Authors conclude that these results may influence upon the thermal injury management strategy in dependence on the trauma effect duration.

Disease Outbreaks↗
PubMedDate not supplied

[Seasonal distribution of intestinal fungal infection by the genus Candida].

To follow-up seasonal distribution of intestinal fungal infection by Candida genus (CGF) in patients and healthy subjects 3786 stool samples have been examined. There were 1302(34.4%) stools of patients and 2484 of healthy subjects of which 421 (16.9%) samples were infected by CGF. In both groups of examinees infection by CGF had a seasonal character. Significantly lower incidence was found within the period January-March (the three-month average percentage of infection was 25.2%, that is, 13.9%) than within the period October-December (the three-month average percentage of infection distribution was 42.7%, that is 20.4%).

Candidiasis↗
PubMedDate not supplied

[The incidence of Staphylococcus saprophyticus in urine and its identification].

Having in mind the known fact that Staphylococcus saproplyticus is one of the most common causes of acute urinary infections, especially in females in the generative period, we have studied its incidence in 12,556 urine samples (6,374 of females and 6,182 of males) taken at the clinics and polyclinics of the Military Medical Academy. For identification of Staphylococcus saprophyticus we have used disk-diffusion test for sensitivity to novobiocin. In order to check realiability of this test we have studied morphological and biochemical characteristics in 30 novobiocin-resistant and 30 novobiocin-sensitive strains of coagulase-negative staphylococci. Of 12,556 examined urine samples we have isolated 217 strains of coagulase-negative staphylococci (10(4) and more bacteria/ml of urine in pure culture). Of this number 33 strains (15.2%) were resistent to novobiocin and we have regarded them as Staphylococcus saprophyticus. The greatest incidence of novobiocin-resistant strains we have found in the urine of female patients treated at polyclinics (19, e.i. 57.6%). Resistence to novobiocin, formation of light yellowish pigment and absence of beta-hemolysis have shown to be satisfactory criteria in diagnosis of Staphylococcus saprophyticus from urine.

Bacteriuria↗
PubMedDate not supplied

[Methicillin-resistant staphylococci. Identification and distribution in the hospital environment].

Occurrence of methicillin resistant staphylococcal strains in hospital environment is more frequently found. These strains are multiresistant so that diseases caused by them require a special therapeutical approach. They are significant causative agents of intrahospital infections which may have epidemic character. Using two methods for detection of methicillin resistant staphylococcus 335 strains were tested. Methicillin resistance was found in 36 (20.6%) of 175 strains of St. aureus and 49 (30.5%) strains of coagulase negative staphylococci. The largest number of methicillin resistant strains is isolated from materials of patients hospitalized at surgical and hematological clinics.

Drug Resistance, Microbial↗