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Biomedical subjects

B King

Publications and source records attributed to B King.

At least 127 records · Page 7Linked to original sources
PubMed1982

Bovine coronavirus structural proteins.

The tissue culture-adapted strain (Mebus) of bovine coronavirus was grown in the presence of isotopically labeled amino acids, glucosamine, or orthophosphate for the purpose of analyzing the virion structural proteins. Five species of polypeptides were identified when purified virions were solubilized in urea and sodium dodecyl sulfate and resolved by polyacrylamide gel electrophoresis. Four species were glycosylated and had apparent molecular weights of 140,000, 120,000, 100,000, and 26,000. The glycoproteins were susceptible to proteolytic cleavage and enzymatic iodination when intact virions were studied and are thus at least partially external to the virion envelope. The 140,000-molecular-weight glycoprotein is apparently a dimer of 65,000-molecular-weight glycopolypeptides held together by disulfide linkages. Species 5 was phosphorylated and had an apparent molecular weight of 52,000. In the intact virion, it was unaffected by protease and was not enzymatically iodinated. It is therefore apparently an internal protein.

Coronaviridae↗
PubMed1979

The use of Doppler shift radar to monitor physiological and drug induced activity patterns in the rat.

Doppler shift radar was used to monitor circadian activity patterns in the rat and to study the behavioural effects of p-chloroamphetamine and d-amphetamine. Activity was classified in two ways:-(a) slow (non-locomotory) and high (locomotory) speed movements. (b) the number of starts of activity, within either the slow or high speed zones of activity during a pre-set time. p-Choloramphetamine (5 mg/kg) produced a biphasic activity response; an initial increase in continuous non-locomotory activity followed by a longer lasting increase in exploratory locomotion containing regular starts of activity. d-Amphetamine (2.5 mg/kg) produced an increase in both non-locomotory and locomotory movements but a marked reduction in starts of activity (i.e. continuous non-exploratory activity). The combination of information on the amount and pattern (starts) of activity allows a more detailed analysis of the effects of drugs on activity to be made than with existing automated methods.

Animals↗
PubMed1977

Antibody-dependent, complement-mediated homologous and cross-cytolysis of togavirus-infected cells.

The appearance of viral induced antigen(s) on Sindbis and Semliki Forest (SF) virus-infected chick embryo cells is detected with homologous mouse anti-viral antibody-dependent, complement-mediated cytolysis in a 51Cr release assay. Such antigen(s) is detected within 3 to 4 hr after infection whereas viral cytopathologic effects do not occur until 10 to 12 hr. In addition, significant heterologous cytolysis (anti-Sindbis on SF virus-infected cells, or, anti-SFV on Sindbis-infected cells) could be observed with serum samples tested. These sera were assayed for neutralizing and hemaggluatination-inhibition activities. Heterologous cytolysis cannot be correlated with hemagglutination-inhibition antibody titers nor is cross-neutralizing antibody detected. The possibility is discussed that another previously undefined antigen on infected cell membranes may be involved in heterologous cytolysis.

Animals↗
PubMed1976

The diabetic day-care unit. I. Development of an index to evaluate diabetes control.

In order to measure the effect of a diabetic day-care unit on diabetes control two scoring scales were constructed, one for insulin-dependent diabetics and the other for patients managed by diet or oral agents or both. Both scales were based on observations of blood and urine glucose concentrations, ketonuria, symptoms of diabetes and deviation from ideal weight. The scale for insulin-dependent diabetics also included the frequency and severity of insulin reactions and frequency of hospital admission for acidosis. Scores for 45 insulin-dependent patients and for 55 diabetics treated by diet with or without oral agents in the unit indicated significant improvement in diabetes control in both groups.

Administration, Oral↗
PubMed1976

Cross-sectional-type weight reference values for village children under five years in Lesotho.

Cross-sectional-type reference values for weight attained are described for village children under five years in rural Lesotho (formerly Basutoland). Weight measurements derive from observations on 1317 children attending an Under-Fives clinic; it is estimated that 60-70% of the children under five in the catchment area were represented. 4585 weighings on boys and 4826 weighings for girls are included in the analysis. Figures of weight-for-age of boys and girls are given separately as centile distributions suitable for use on Growth Charts. Lesotho 50 centile approximates to 3 centile of British children and slightly exceeds 80% Harvard standard. Weight attained for age is similar, in both sexes, to reports from other less-priviledged urban and rural areas, emphasizing the relative importance of environmental as compared to genetic influences in determining weight-for-age in early childhood. It is suggested that the construction of locally-derived growth reference values is both appropriate and practicable.

Age Factors↗