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Biomedical subjects

B Klinge

Publications and source records attributed to B Klinge.

At least 73 records · Page 4Linked to original sources
PubMed1989

Location of the mandibular canal: comparison of macroscopic findings, conventional radiography, and computed tomography.

Four mandibular specimens were radiographically examined bilaterally to locate the mandibular canal. The following radiographic techniques were used: periapical and panoramic radiography, hypocycloidal tomography, and computed tomography (CT). The distance from the crest of the alveolar process to the superior border of the mandibular canal was measured in millimeters on all radiographs. The specimens were then sectioned, and the location of the mandibular canal (as measured on contact radiographs of the sections) was compared with measurements made on the other radiographs. The results showed that CT gave the most accurate position of the mandibular canal and is therefore probably the best method for preoperative planning of the implant surgery involving the area close to the mandibular canal.

Humans↗
PubMed1988

Long-term retention of TiF4 and SnF2 after topical application to dentin in dogs.

As part of an evaluation of the potential of topical application of TiF4 in the prevention or arrest of root caries, the longevity of the titanium-rich glaze and the long-term retention of titanium and fluoride in exposed dentin surfaces were examined in dogs. For comparison, the effect of fluoride in combination with another cation, tin, was studied as well. Facets of exposed dentin were prepared in two beagles and treated for 1 or 4 min with either a TiF4 or an SnF2 solution (1.1 M F, native pH). After 3, 7, or 21 wk, the experimental surfaces were analyzed for fluoride, titanium, tin, and calcium using an electron microprobe. Dentin surfaces treated with TiF4 for 1 min showed fluoride concentrations ranging from less than 0.15% to 1.03%, while those treated for 4 min with SnF2 showed values from 0.22% to 1.28%. The calcium analyses revealed partial surface demineralization in one half of the SnF2-treated specimens, while this was a rare finding in the TiF4 group. The findings show that a brief application of TiF4 to exposed dentin results in approximately the same increase in fluoride content as a 4-min application of SnF2, with, however, less surface demineralization. In addition, the presence of a surface glaze may impart an added benefit to the use of TiF4 as a topical agent in the prevention of root caries.

Animals↗
PubMed1987

Effects of bone wax on rabbit cranial bone lesions.

The present study was designed to elucidate the reactions of cranial membranous bone to bone wax. In ten young rabbits, twenty parietal bone defects were created by drilling, the edges of which were partly extended using rongeur forceps to enable investigation of eventual thermal effects. Half of the marginal bone surrounding the lesions was covered by bone wax, the remainder serving as control. The animals were sacrificed 1 and 7 weeks after surgery, and block specimens prepared for light microscopy. Merely slight tissue reactions to the bone wax were discerned. Bony regeneration occurred mainly from the dura mater and the pericranium, but also from the bony rim. Reskeletalization was markedly impaired by the presence of bone wax. Heat generated by drilling caused reduced bone formation despite constant irrigation peroperatively. Clinical consequences are discussed.

Animals↗
PubMed1987

New views on the construction of human gingival epithelium.

The effects on the basal and spinous layers of human keratinized oral epithelium of 2 glutaraldehyde-based fixatives with buffers hypoosmolar and isoosmolar to blood, respectively, have been investigated. The first-mentioned solution produced an electron microscopic image corresponding to the classical view, that is an epithelium consisting of closely-packed cells having short and stubby membrane projections and separated by an extremely narrow intercellular space. In the Langerhans cells, the specific granules appeared racket-shaped and had a unilaminar limiting membrane with a periodic structure along its internal face. There are strong reasons to believe that these morphological characteristics are swelling artifacts. The last-mentioned fixative produced keratinocytes provided with numerous microvilli and membrane ruffles and disk-shaped Langerhans cell granules surrounded by a trilaminar membrane. Concomitantly, the intercellular space appears very wide and there is evidence for the view that this is a more realistic picture of the in vivo situation and not a gross distortion caused by shrinking during the tissue processing. Broad interstices can explain certain basic events as rapid cell motility within the epithelium and offer efficient pathways for rapid transport of substances.

Adult↗
PubMed1985

Effect of periodic tooth displacement on healing of experimental furcation defects in dogs.

This study compared the healing response, following reconstructive surgery, between teeth subjected to periodic displacement and teeth which were splinted during the postoperative healing period. Through-and-through furcation defects were created in the mandibular second, third, and fourth premolars of 6 beagle dogs. Bone was surgically removed from the furcation and around each root to 3 different levels: 3, 6, and 9 mm. Reconstructive surgery included demineralization of the root surface and coronally positioned flaps. During the postoperative healing period, the premolars on one side were subjected to periodic mesio-distal displacement by orthodontic elastics attached to splints. Corresponding teeth on the contralateral side were splinted to serve as non-mobile controls. The results demonstrated that reattachment occurred in 12 of 14 displaced teeth and in 14 of 16 splinted teeth. There was no difference in the amount of interradicular bone fill or frequency of ankylosis between displaced and splinted teeth. Our findings indicate that the healing response was not affected by tooth displacement in this dog furcation model.

Alveolar Process↗
PubMed1985

Effect of crown-attached sutures on healing of experimental furcation defects in dogs.

This study evaluates whether suturing of replaced flaps with crown-attached sutures, following reconstructive surgery, counteracts postoperative recession of the gingival margins and facilitates new attachment. Through-and-through furcation defects were created in the mandibular premolars of 5 beagle dogs. Bone was surgically removed from the furcation and around each root to a level 3 to 4 mm below the cemento-enamel junction. Steel wires were placed in the furcations for 6 weeks to enhance plaque formation. After 12 weeks, reconstructive surgery that included acid conditioning of root surfaces was performed. On 1 side of the mandible, the flaps were replaced and secured by bonding the sutures to the teeth with composite resin. On the other side, the replaced flaps were sutured interdentally with single sutures. The results demonstrated that the margins of flaps secured with interdental sutures shifted apically to the fornix of the furcations after 1 week, and histologically all 10 furcations were found to be lined with epithelium. Margins of flaps secured with crown-attached sutures remained an average of 0.5 mm coronally to the fornix, and 13 of the 14 teeth showed new attachment. The results indicate that a suturing technique which counteracts gingival recession, favours the formation of new attachment in this beagle dog model.

Animals↗
PubMed1985

Bone regeneration pattern and ankylosis in experimental furcation defects in dogs.

This study reports on the pattern of interradicular bone formation and the frequency and localization of ankylosis following regenerative surgery of experimentally-induced furcation defects in 12 beagle dogs. Observations were made in 39 specimens showing healing with connective tissue attachment in the full circumference of the furcation. The findings indicate that bone regeneration first occurs adjacent to the root surfaces, followed by a subsequent fill of the central portion of the interradicular area. Ankylosis, observed in 24 of the 39 specimens, was more frequent in large defects than in small defects. The incidence of ankylosis increased with increased distance from the base of the original wound. These findings suggest that coronal bone apposition may require or be facilitated by the presence of a root surface, and that undisturbed coronal regeneration may be dependent upon cellular or some other activity from the intact periodontal ligament.

Animals↗
PubMed1985

Effect of implants on healing of experimental furcation defects in dogs.

The present study was performed to find out whether implants placed around periodontally involved teeth during reconstructive surgery would support replaced flaps and prolong gingival coverage of the treated root surfaces facilitating new attachment. Through-and-through furcation defects were made in the mandibular second, third, and fourth premolars of 10 beagle dogs. Bone was surgically removed from the furcation and from the circumference of each tooth to a level 3 to 4 mm apical to the cementoenamel junction. Steel wires were passed through the furcations and ligated to enhance plaque formation. At 6 weeks, the wires were removed. At 12 weeks, during reconstructive surgery, 4 dogs received implants of autogenous intraoral cancellous bone, 2 of autogenous iliac cancellous bone and marrow, 2 of Proplast, and 2 of Avitene. The results demonstrated that none of 12 teeth implanted with Avitene or Proplast showed new attachment. Only 2 of 6 teeth implanted with autogenous iliac bone showed new attachment, combined with extensive root resorption and ankylosis. Of 12 teeth implanted with autogenous intraoral bone, 6 showed new attachment. Our findings suggest that flap support by intraoral cancellous bone implants may facilitate new attachment. A more predictable technique, however, needs to be developed.

Animals↗
PubMed1985

Effect of dental amalgam restorations on the mercury content of nerve tissues.

In an autopsy study in two men and an experimental study performed on three female beagles the mercury burden of nerve tissues was determined. Nerve tissues from the head and face region and from three peripheral nerves were analyzed for mercury content with the aid of atomic absorption spectroscopy. In the dogs dental amalgam restorations were placed so as to investigate the possible influence from the amalgam on the mercury content of the tissues under study. The mercury content in man and dogs differed widely from one nerve to another, with no apparent relation to the number, type, or location of tooth restorations.

Aged↗
PubMed1984

The effect of citric acid on repair after delayed tooth replantation in dogs.

An experimental study of maxillary incisors in dogs was carried out to test the hypothesis that demineralization of denuded root surfaces by citric acid to expose collagen matrix fibrils might improve the rate of periodontal regeneration after replantation of traumatically avulsed and dried teeth, provided that the root surface is not traumatized during replantation or postoperatively. Experimental teeth (group 4) were allowed to dry in air for 45 min. A mid-portion of the root was then root-planed extensively, treated with citric acid for 3 min, rinsed with saline, and replanted. The apical region and a cervical collar were not instrumented, ensuring precise reseating and stability of the tooth during the postoperative period. Control teeth were either replanted immediately (group 1), bench-dried for 45 min and replanted (group 2), or bench-dried and the entire root surface root-planed and acid-treated before replantation (group 3). Histological examination of block specimens after 21 days of healing showed complete periodontal regeneration in group 1 and, by contrast, a high rate of ankylosis, inflammatory resorption, and surface resorption in group 2. Root planing and acid treatment of bench-dried teeth (group 3) did not improve the healing response, whereas the mid-root region of teeth in group 4 showed an absence of inflammatory resorption and less ankylosis than groups 2 and 3. These preliminary observations indicate that the frequency of adverse healing results after delayed replantation can be reduced by removing nonvital soft tissue remnants, demineralizing the root surface, and preventing mechanical trauma to the root surface in the postoperative period.

Animals↗
PubMed1983

Histopathology of initial gingivitis in humans. A pilot study.

The present study concerns the inflammatory alterations in the gingival margin during initial gingivitis in 11-13 year old human subjects. At day 0 of the experiment, all participants had clean teeth and healthy gingiva. All active oral hygiene measures were excluded for 4 days. From upper and lower premolars, which were extracted for orthodontic reasons, contralateral gingival biopsies, including the tooth and the adjacent gingiva, were obtained on days 0 and 4. The presence of inflammatory cells in the junctional epithelium and the adjacent connective tissue was determined quantitatively in semi-thin sections. The collagen content of the gingival margin was also determined. From day 0 to day 4 there was only a slight increase in the number of neutrophilic granulocytes in the junctional epithelium and adjacent connective tissue, while a more pronounced increase was found in the number of mononuclear leukocytes. A loss of collagen was noticed in 4 of the subjects, while 2 did not show any changes in collagen content. The inflammatory reaction seen in the present study differs somewhat from that observed in adult humans and adult dogs. The results correspond more to the reaction seen in juvenile dogs.

Adolescent↗