Biomedical subjects
Publications and source records attributed to B Klingeborn.
Antibody to equid herpesvirus 1, which mediates single radial hemolysis, is that responsible for neutralization. Hemagglutination inhibition antibody is not necessarily involved in neutralization or hemolysis.
Explore the source record for details and available documents.
Six cases of paresis occurred in a Swedish stud with 48 mares and a stallion. Complement-fixation tests revealed a recent infection with EHV-1 in most horses of the stud. Serumneutralisation tests showed rapid antibody-titre increases during the course of the disease. This type of antibody response was interpreted as induced by reinfection or, possibly, recurrent infection. Two diseased mares were sacrificed. No virus could be isolated from their central nervous system (CNS), liver or spleen, but there is a presumptive evidence for the presence of an antigen specific to EHV-1 in the CNS and liver. Neutralising antibodies to EHV-1 were demonstrated in the liver and kidneys following elution by acidification of the tissues. No such antibodies could be demonstrated in the brain and spinal cord. A possible reason for this failure is discussed.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Twelve mares were vaccinated with attenuated equine abortion virus (EAV) strain RAC-H. Two nonvaccinated mares served as controls. In at least three mares the vaccination appeared to coincide with a natural infection. This was indicated by characterization of the EAV isolated from nasal secretions of six vaccinated mares, a nonvaccinated control, and also from the lung, spleen, and liver of a fetus aborted by a vaccinated mare. The relative sensitivity of the isolated EAV to dithiothreitol was used to distinguish the RAC-H strain and wild-type virus. Of the 10 EAV isolates, four were recognized as being the vaccine strain while six were recognized as being wild-type strains. Three of the latter originated from two vaccinated mares and a nonvaccinated control, and three were recovered from the fetus. The ability of the EAV strains to form plaques in a cloned line of L cells proved to be unsuitable for use as a marker in this study.
Explore the source record for details and available documents.
The infectivity of equine abortion (herpes) virus (EAV) was inactivated by treatment with reduced dithiothreitol (DTT). According to their susceptibility to DTT, the EAV strains could be divided into three groups. The vaccine strain RAC-H (419) proved to be more resistant to DTT than all of the other 14 strains tested. The hemagglutinin of EAV was also inactivated by DTT; no strain differences were observed in this respect.
Explore the source record for details and available documents.