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Biomedical subjects

B Korych

Publications and source records attributed to B Korych.

At least 19 recordsLinked to original sources
PubMed1993

[Strategy of viral replication].

Virus replication is a multistep process which is realized mostly by virus-coded enzymes and in a small part by cell-coded enzymes. Affection of the virus-coded enzymes can be, and is, utilized to restrict virus replication without damaging the host cell. The article provides basic information on the most important steps of viral replication and processes which can be affected in the course of antiviral therapy.

Virus Replication↗
PubMed1990

Local and systemic antibody response in infants after oral administration of inactivated enteropathogenic E. coli serotype O111 and O55.

In ten infants divided into two groups (up to one month of age and at 2-7 months of age) the dynamics and formation of different antibody isotypes produced locally in the intestine and in serum after orally administered inactivated enteropathogenic E. coli strains O111 and O55 was followed during 30 d after the first and booster dose by using an indirect immunofluorescence method. Infants up to one month of age produced antibodies of IgM isotype in stool together with the IgA isotype after the first and booster dose of the vaccine against both antigens. Serum IgG antibody increased after 2 d following the first and second dose of antigens and remained higher during 5 d. The infants aged 2-7 months expressed predominantly the IgA isotype response in stool after the first and booster dose of antigens. The serum immunoglobulin levels did not change after oral antigen administration.

Adhesins, Escherichia coli↗
PubMed1983

Biological activities of enterotoxin from Vibrio cholerae non-01.

The specific antiserum 54(2) prepared in rabbits by combined intraperitoneal and intravenous immunization with the enterotoxin from Vibrio cholerae non-01 was found to neutralize the enterotoxin biologic activity expressed by rabbit skin vascular permeability and hemorrhage factors. The inhibition of biologic activity was also observed when using antiserum to choleragen, but choleragen could not be neutralized by specific serum 54(2). The enterotoxin was also inactivated when heated at 100 degrees C for 10 min, but heating at 56 degrees C for 30 min had no influence on its biologic activity. The enterotoxin from Vibrio cholerae non-01 was found to elevate concentration levels of cAMP in the epithelial cells of the rabbit intestine and this elevation was comparable with that produced by the filtrate of Vibrio cholerae 569 B culture. The culture filtrate from Vibrio cholerae non-01 exhibited a similar activity. The Vibrio cholerae non-01 enterotoxin as well as the original culture filtrate were found to be cytotoxic for Vero cells.

Adenylyl Cyclases↗
PubMed1982

Toxic factors of Aeromonas hydrophila and Plesiomonas shigelloides.

In detail, exoproducts of Aeromonas hydrophila and Plesiomonas shigelloides were studied on the pathogenesis of diarrhoea. Enterotoxin, hemolysin and a cytotoxic factor were found in a fraction of culture filtrates of Aeromonas hydrophila with a molecular weight of approximatley 60,000. Apart there from a cytotoxic factor was also found in a fraction with molecular weight above 100,000. This fraction contained also endotoxin which was hemolytic, too. Enterotoxic activity was expressed by an accumulation of fluid in the ligated ileal loop and by increased vascular permeability in the skin of rabbit. Activation of adenylate cyclase was not found. Cytotoxic activity was represented by hemorrhagic reaction in the skin of rabbit, in some cases by sanguinous and mucous fluid in the ligated ileal loop and by significant morphological alterations on Vero cells leading to the death of cells. Culture filtrates of Plesiomonas shigelloides show only very low activity in biological experiments. Separation and purification steps led to a more concentrated and active material.

Aeromonas↗