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Biomedical subjects

B L Beard

Publications and source records attributed to B L Beard.

17 recordsLinked to original sources
PubMed2006

Thermal inactivation studies of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes in ready-to-eat chicken-fried beef patties.

Thermal inactivation studies were used to determine the D- and z-values of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes in ready-to-eat chicken-fried beef patties. Inoculated meat was packaged in sterile bags, which were immersed in a circulated water bath and held at 55, 57.5, 60, 62.5, 65, 67.5, and 70 degrees C for different lengths of time. D- and z-values were determined with a linear regression model. Average D-values at temperatures 55 to 70 degrees C were 27.62 to 0.04 min for E. coli 0157:H7, 67.68 to 0.22 min for Salmonella, and 81.37 to 0.31 min for L. monocytogenes. The z-values were 5.2 degrees C for E. coli O157:H7, 6.0 degrees C for Salmonella, and 6.1 degrees C for L. monocytogenes. The results of this study can be used by food processors to validate their processes and help eliminate pathogenic bacteria associated with chicken-fried beef products.

Animals↗
PubMed2004

Thermal process validation for Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes in ground turkey and beef products.

At 55 to 70 degrees C, thermal inactivation D-values for Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes were 19.05 to 0.038, 43.10 to 0.096, and 33.11 to 0.12 min, respectively, in ground turkey and 21.55 to 0.055, 37.04 to 0.066, and 36.90 to 0.063 min, respectively, in ground beef. The z-values were 5.73, 5.54, and 6.13 degrees C, respectively, in ground turkey and 5.43, 5.74, and 6.01 degrees C, respectively, in ground beef. In both ground turkey and beef, significant (P < 0.05) differences were found in the D-values between E. coli O157:H7 and Salmonella or between E. coli O157:H7 and L. monocytogenes. At 65 to 70 degrees C, D-values for E. coli O157:H7, Salmonella, and L. monocytogenes were also significantly (P < 0.05) different between turkey and beef. The obtained D- and z-values were used in predicting process lethality of the pathogens in ground turkey and beef patties cooked in an air impingement oven and confirmed by inoculation studies for a 7-log (CFU/g) reduction of E. coli O157:H7, Salmonella, and L. monocytogenes.

Animals↗
PubMed2003

Determination of thermal lethality of Listeria monocytogenes in fully cooked chicken breast fillets and strips during postcook in-package pasteurization.

Fully cooked chicken breast fillets and strips were surface inoculated with a cocktail of Listeria monocytogenes culture. The inoculation level was 10(7) to 10(8) CFU/g meat. The inoculated products were vacuum packaged and pasteurized at 90 degrees C with a pilot-scale steam or hot water cooker. After heat treatment, the survivors of L. monocytogenes were enumerated. No significant difference was found on survivors of L. monocytogenes between steam- and hot water-treated products. To achieve a 7-log10 (CFU/g) reduction, approximately 5, 25, and 35 min were needed for single-packaged fillets, 227-g package strips, and 454-g strips, respectively. The results from this study were subsequently verified by a computer model that could predict the thermal lethality of pathogens in fully cooked meat and poultry products during postcook in-package pasteurization.

Animals↗
PubMed2003

Thermal inactivation of Listeria monocytogenes on ready-to-eat turkey breast meat products during postcook in-package pasteurization with hot water.

The inactivation of Listeria monocytogenes during postcook in-package pasteurization was evaluated for fully cooked turkey breast meat products (4-kg packages). The products were surface-inoculated to contain 10(7) CFU of L. monocytogenes per cm2 of product surface. The inoculated products were vacuum-packaged in different thicknesses (0.08 to 0.33 mm) of packaging films and treated with hot water at 96 degrees C. After heat treatment, the products were immediately cooled in an ice water bath at 0 degrees C. The relationship between heating time and product surface temperature was determined for different thicknesses of packaging films. The effectiveness of heat treatment for inactivating the pathogen was affected by product surface roughness. About 50 min of heating time was needed to achieve a thermal kill of 7 log10 CFU/cm2 on products with surface roughness up to 15 mm in depth. The cooling time needed after a heat treatment increased with an increasing endpoint temperature of the heated product and the heat penetration depth reached in the product. The cooling time needed to cool the product from 71 degrees C to 4 degrees C was about 2.5-fold the heating time.

Animals↗
PubMed2000

Strontium isotope composition of skeletal material can determine the birth place and geographic mobility of humans and animals.

The Sr isotope composition measured in skeletal elements (e.g., bone, teeth, or antlers) can be used to infer the geographic region that an animal or human inhabited, because different regions tend to have distinct Sr isotope compositions, and natural variations in the relative abundance of Sr isotopes are not changed as Sr is processed through the food chain. Therefore, an organism that ingests Sr from one region can have a Sr isotope composition that is different than that of an organism that ingests Sr from another region. The Sr isotope composition of skeletal elements is a reflection of the concentration-weighted average of dietary Sr that was ingested while that skeletal element was produced. Because different skeletal elements grow and exchange Sr at different stages during the life times of organisms, Sr isotope analysis of different skeletal elements can be used to infer changes in geographic location at different stages in an organism's life. The Sr isotope composition measured in human teeth will reflect the average Sr isotope composition that was ingested as a child, due to the immobile nature of Sr and Ca in teeth after formation, whereas the Sr isotope composition of bone will reflect the average isotopic composition over the last ten years of life, due to continuous biological processing of Sr and Ca in bone. Inferring the average isotopic composition of dietary Sr is best done by analyzing skeletal fragments from control groups, which might be animals that have the same feeding habits as the animal in question, or, in the case of humans, analysis of close family relatives. In cases where it is not possible to construct a Sr isotope database from control groups, it becomes necessary to estimate the isotopic composition of dietary Sr based on geologic principles. We present three case studies from our research that illustrate a range of approaches: (1) results from a criminal case where a deer was illegally harvested and the location of the deer was important to establish, (2) a pilot study of commingled human remains from a burial in Vietnam, associated with the Vietnam Conflict, and (3) a study of 13th and 14th century migration of peo ple from an archeological site in the Southwest United States.

Adult↗
PubMed1999

Iron isotope biosignatures.

The (56)Fe/(54)Fe of Fe-bearing phases precipitated in sedimentary environments varies by 2.5 per mil (delta(56)Fe values of +0.9 to -1. 6 per mil). In contrast, the (56)Fe/(54)Fe of Fe-bearing phases in igneous rocks from Earth and the moon does not vary measurably (delta(56)Fe = 0.0 +/- 0.3 per mil). Experiments with dissimilatory Fe-reducing bacteria of the genus Shewanella algae grown on a ferrihydrite substrate indicate that the delta(56)Fe of ferrous Fe in solution is isotopically lighter than the ferrihydrite substrate by 1.3 per mil. Therefore, the range in delta(56)Fe values of sedimentary rocks may reflect biogenic fractionation, and the isotopic composition of Fe may be used to trace the distribution of microorganisms in modern and ancient Earth.

Earth, Planet↗
PubMed1999

Detection in fixed and random noise in foveal and parafoveal vision explained by template learning.

Foveal and parafoveal contrast detection thresholds for Gabor and checkerboard targets were measured in white noise by means of a two-interval forced-choice paradigm. Two white-noise conditions were used: fixed and twin. In the fixed noise condition a single noise sample was presented in both intervals of all the trials. In the twin noise condition the same noise sample was used in the two intervals of a trial, but a new sample was generated for each trial. Fixed noise conditions usually resulted in lower thresholds than twin noise. Template learning models are presented that attribute this advantage of fixed over twin noise either to fixed memory templates' reducing uncertainty by incorporation of the noise or to the introduction, by the learning process itself, of more variability in the twin noise condition. Quantitative predictions of the template learning process show that it contributes to the accelerating nonlinear increase in performance with signal amplitude at low signal-to-noise ratios.

Artifacts↗
PubMed1998

Orientation dependency for foveal line stimuli: detection and intensity discrimination, resolution, orientation discrimination and vernier acuity.

Thresholds were measured for five tasks: line detection, intensity discrimination, two-line resolution, vernier acuity and line-orientation discrimination. For each task, 30 arcmin lines were presented foveally in eight retinal meridians to assess similarities in orientation anisotropies across tasks in the same observer. Three observers were tested. The pattern of the orientation anisotropy differs across tasks. Meridional anisotropies exist in detection, increment discrimination thresholds, and vernier acuity but the classical oblique effect is consistently found only in orientation discrimination.

Discrimination, Psychological↗
PubMed1997

Vernier acuity with non-simultaneous targets: the cortical magnification factor estimated by psychophysics.

The eccentricity at which peripheral thresholds double their foveal value (E2) may relate to the visual system's anatomical organization. Using a variety of experimental approaches, previous estimates of E2 for vernier acuity have ranged from less than 0.1 deg to greater than 15.0 deg. This broad range of values seems to challenge the usefulness of E2 for determining visual topography. We explain that the varying contributions from at least two different regimes, spatial filter and local sign, may explain the broad range of E2 values found previously. We attempt to limit responses to the local sign regime, where it may be possible to determine the psychophysical analog to the gradient of the cortical spatial grain. In our experiments we measure how vernier task performance falls off with eccentricity. We hypothesize that if the vernier features are adequately separated in time, they will fall outside of the spatial filter's temporal integration span and the local sign regime would then predominate for precise positional processing. Using an interstimulus interval ranging from 20 to 200 msec between the two vernier features, we estimate that vernier thresholds in the local sign regime double at about 0.8 +/- 0.2 deg eccentricity, which is similar to anatomical estimates of the eccentricity at which the linear spacing of human cortical units doubles.

Contrast Sensitivity↗
PubMed1997

Motion thresholds can be predicted from contrast discrimination.

The detection thresholds for oscillatory motion and flicker were compared across a wide range of pedestal contrasts, spatial frequencies, and temporal frequencies in both foveal and peripheral vision. Motion and flicker stimuli were both generated by summation of a counterphase test grating with a static pedestal grating. For the oscillatory motion task the test and the pedestal were presented 90 deg out of phase to each other, whereas for flicker the two gratings were presented in phase. Since detection of both stimuli would depend on the same test stimulus, detection thresholds could be similar even though the tasks differ. Although there was a slight elevation in flicker detection thresholds compared with motion thresholds, our main finding is that oscillatory motion and flicker thresholds for suprathreshold sinusoidal gratings are similar. This finding supports the idea that motion and flicker have a common underlying detection mechanism. Our finding that flicker thresholds are slightly higher than jitter thresholds indicates that the contrast gain control (or saturating energy transducer) has a weak phase dependence. The ability to discriminate motion from flicker was elevated relative to their detection thresholds, particularly at high temporal frequencies. We offer two models to account for this behavior. The discrimination of motion from flicker may require a temporal comparison of the outputs of directionally selective filters tuned to opposite directions or to the population statistics of a bank of separable mechanisms.

Contrast Sensitivity↗
PubMed1997

Image discrimination models predict detection in fixed but not random noise.

By means of a two-interval forced-choice procedure, contrast detection thresholds for an aircraft positioned on a simulated airport runway scene were measured with fixed and random white-noise masks. The term fixed noise refers to a constant, or unchanging, noise pattern for each stimulus presentation. The random noise was either the same or different in the two intervals. Contrary to simple image discrimination model predictions, the same random noise condition produced greater masking than the fixed noise. This suggests that observers seem unable to hold a new noisy image for comparison. Also, performance appeared limited by internal process variability rather than by external noise variability, since similar masking was obtained for both random noise types.

Discrimination, Psychological↗
PubMed1995

Perceptual learning in parafoveal vision.

The present study tests the effects of practice on parafoveal vernier and resolution acuity. By measuring task specificity, transfer of training to other retinal locations in the trained eye and transfer of training to the untrained eye, we directly address whether improvement on these tasks is the result of changes in the underlying physiological processes or simply the development of new cognitive strategies. We found that: (1) significant learning can occur for both vernier and resolution acuity in many (but not all) individuals; (2) there were significant individual differences in the degree and time-course of learning: (3) learning transfers to the untrained task; and (4) learning transfers to the other eye particularly when the visual pathway leads to the trained hemisphere. These results suggest that both physiological and cognitive processes contribute to the improvement seen after repetitive practice on these visual tasks.

Adolescent↗
PubMed1994

Spatial scale shifts in amblyopia.

We used a masking paradigm to uncover the properties of the mechanisms engaged by the amblyopic visual system for vernier acuity and line detection. Line vernier and line detection thresholds were measured in the presence of one-dimensional noise masks varying in orientation, spatial frequency content or contrast. Our results reveal that in both normal and amblyopic eyes, there is a bimodal orientation tuning function for vernier acuity, i.e. vernier acuity is most strongly masked by mask orientations approx. +/- 10 deg on either side of the target lines. In contrast, in both normal and amblyopic eyes, line detection is most strongly masked when the mask and line target have the same orientation. In the normal fovea, the spatial frequency tuning is bandpass, with a peak spatial frequency of about 10 c/deg. In the amblyopic eyes, the spatial tuning is similar in specificity; however the peak is shifted to lower spatial frequencies, suggesting a shift in the scale of spatial processing of line stimuli. For all of the amblyopic eyes, the increased line detection thresholds are approximately proportional to the shift in spatial scale. In anisometropic amblyopes, the (unmasked) vernier threshold is elevated in proportion to the shift in spatial scale; however in some amblyopes with constant strabismus the shift in spatial scale is not sufficient to account for the degraded vernier acuity. The "extra" increase in vernier thresholds associated with strabismus may be a consequence of a high degree of positional uncertainty which adds noise at a stage following the combination of filter responses.

Adult↗
PubMed1994

Age differences in spatial contrast sensitivity are not the result of changes in subjects' criteria or psychophysical performance.

PURPOSE: To analyze separately the sensory and nonsensory aspects of reductions in contrast sensitivity with age. METHODS: Two-alternative forced choice psychometric functions were fitted with a three-parameter Weibull function, which independently estimated threshold, slope, and upper asymptote of the functions. RESULTS: There were no changes in slope or upper asymptote with age, but contrast sensitivity decreased with age. CONCLUSIONS: Most studies of decreased visual function in the elderly are difficult to interpret because possible nonsensory effects are ignored. This paper illustrates a method that can be used to demonstrate a true sensory decline with age.

Adult↗
PubMed1994

Contrast detection and discrimination in young and older adults.

PURPOSE: To determine if age differences in contrast detection thresholds extend to suprathreshold contrast discrimination. METHODS: Psychophysical contrast detection and discrimination thresholds were determined in 56 adults ranging in age from 22 to 72 years. In experiment 1, thresholds were measured using a two-interval forced-choice procedure across a range of pedestal grating contrasts. In experiment 2, detection and discrimination thresholds were measured at two spatial frequencies and two luminance levels. RESULTS: When normalized to the contrast detection threshold, contrast discrimination thresholds were similar in young and older adults. This result is akin to previous findings for clinical patients with contrast detection deficits. In addition, contrast discrimination in the elderly is independent of mean display luminance as has been found in young adults. CONCLUSIONS: Normalized contrast discrimination functions have the same shape in young and older adults and show no change with a 4-fold reduction in luminance.

Adult↗
PubMed1988

Age and visual search: expanding the useful field of view.

The useful field of view is defined as the visual area in which information can be acquired within one eye fixation. We studied visual search within this context and found a reduction in the size of the field as a function of age. This loss, however, was recovered partially with practice. Standard acuity and perimetric tests of visual field, although diagnostic of disease, underestimate the degree of difficulty experienced by visually healthy older adults in everyday activities requiring the use of peripheral vision. To aid in predicting such performance, a model incorporating the effects of distractors and secondary task demands was developed.

Adult↗