Biomedical subjects
Publications and source records attributed to B L Lamberts.
Root surfaces which have lost their attachment due to chronic inflammatory periodontal disease present significant increases in calcium, magnesium, phosphorus, and fluoride. The clinical significance of this effect is not known in regards to wound healing, but it may be helpful in control of cervical sensitivity.
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Intervase from extracellular culture fluids of S. mutans strain SL-1 was shown to have the same characteristics as intracellular invertase from the same strain. The data indicate that intracellular invertase is released into the culture fluids primarily during the late log and stationary phases of growth.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
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The solubilities of extracellular polysaccharide fractions produced by 14 oral streptococcal strains were compared in water, aqueous lithium and guanidine salt solutions, dimethyl sulfoxide (DMSO), and a 9:1 DMSO-water mixture. The best results for solubilizing the fractions were obtained with the DMSO-water mixture procedure, which used the water and DMSO in sequence. By this method all fo the fractions were solubilized.
This study explored the use of acrylamide gel electrophoretic methods to determine the numbers and types of extracellular sucrose-metabolizing enzymes produced by particular strains of S mutans. Strains HS-6, SL-1, FA-1, and NCTC 10449 were cultured in a chemically defined medium and the extracellular proteins elaborated by the organisms were isolated and subjected to acrylamide gel electrophoresis. Patterns of protein components and sucrose-metabolizing enzymes were then delineated. Three types of sucrose-metabolizing enzymes were observed. One type was involved in the synthesis of polysaccharides that were insolubilized in the gels. Another type was involved in the synthesis of water-soluble polysaccharides. A third type was involved in the splitting of sucrose into reducing sugars without polysaccharide synthesis. Each pattern was distinctive with regard to the numbers, proportions, and types of enzyme components present and their migratory characteristics. From two to at least six components were observed amont the enzyme activity patterns per strain. Extracellular protein patterns showed from 12 to 20 components per strain. Comparative data on growth in the chemically defined medium and Todd-Hewitt broth were also presented. Better growth levels were obtained in all instances with the chemically defined medium over comparable periods of time.
Enamel solubility rates were determined in vivo to depths of 1 to 2 mum in the teeth from two samples of naval recruits that had been grouped, respectively, according to DMFT scores and visual indexes of oral hygiene status. No positive correlation between enamel solubility rates and DMFT scores were observed; rather there was a tendency for caries-resistant participants to have a highest apparent enamel solubility rates, even though mean differences among the DMFT groups were not generally significant. Intra-subject measurements on 12 teeth per participant for eight of the recruits showed, on the average, only about one half the variability founl incisors for more than 70 of the recruits. The least variability was found for measurements on paired maxillary central incisors. It was concluded that factors such as tooth morphology and, in particular, tooth-surface films could considerably affect enamel solubility rates measured in vivo.