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Biomedical subjects

B L Rogers

Publications and source records attributed to B L Rogers.

47 records · Page 3Linked to original sources
PubMed1989

School Breakfast Program and school performance.

To test the hypothesis that participation in the School Breakfast Program by low-income children is associated with improvements in standardized achievement test scores and in rates of absence and tardiness, children in grades 3 through 6 were studied in the Lawrence, Mass, public schools, where the School Breakfast Program was begun at the start of the second semester 1986-1987 school year. The changes in scores on a standardized achievement test and in rates of absence and tardiness before and after the implementation of the School Breakfast Program for children participating in the program were compared with those of children who also qualified but did not participate. Controlling for other factors, participation in the School Breakfast Program contributed positively to the 1987 Comprehensive Tests of Basic Skills battery total scale score and negatively to 1987 tardiness and absence rates. These findings suggest that participation in the School Breakfast Program is associated with significant improvements in academic functioning among low-income elementary school children.

Absenteeism↗
PubMed1989

Foodservice trends in the elderly nutrition program.

The foodservice delivery aspects of the federally-funded Elderly Nutrition Program (ENP) were examined via an original survey instrument sent to a random sample of nutrition projects nationally. In comparison to a similar survey conducted a decade ago, projects were more apt to rely on a combination of foodservice mechanisms including caterer contracts, on-site preparation, and use of central kitchens.

Aged↗
PubMed1987

Expression of the T-cell receptor gamma-chain gene products on the surface of peripheral T cells and T-cell blasts generated by allogeneic mixed lymphocyte reaction.

The gamma-chain genes of the T-cell receptors form a family of related genes that are specifically expressed and somatically rearranged in T cells. Using poly- and monoclonal anti-gamma antibodies, we studied the cell-surface expression of the gamma-chain gene products in mouse peripheral T cells as well as in the T-cell blasts generated by allogeneic mixed lymphocyte reactions. The gamma chains are expressed in the Lyt2-,L3T4- subsets of these T-cell populations as disulfide-linked heterodimers. Whereas the electrophoretic mobility and the N-glycosylation of the spleen and lymph-node gamma chains are indistinguishable from those of the reported thymocyte gamma chain, a minor fraction of the T blasts generated by allogeneic stimulation of B10 lymph-node T cells with B10.BR spleen cells seems to express gamma chains with distinct properties. This suggests that the mixed lymphocyte culture conditions exert a selective effect on the expression of gamma chains among peripheral T-cell populations.

Animals↗
PubMed1986

Transcriptional enhancer within the human placental lactogen and growth hormone multigene cluster.

Human placental lactogen (hPL) and human growth hormone (hGH) are members of a multigene family that share amino acid sequence homology and similarity in gene structure and nucleotide sequence, but differ in both function and expression. To determine the sequence requirements for tissue specific expression recombinant plasmids containing the members of the hPL-hGH multigene family and flanking regions were analyzed by both transient and stable transfection assays. We have identified a transcriptional enhancer in a 1.0 kb region located 2.0 kb downstream of the hPL3 structural gene. This enhancer sequence is not strictly cell-type specific since it functions in cell lines of both placental (JEG-3) and pituitary (18-54,SF) origin. However, its efficiency is several fold higher in placental cells than in pituitary cells.

Base Sequence↗
PubMed1986

Synthesis and biological activity of derivatives of ubiquinone: photoaffinity analogues containing the 4-azido-2-nitroanilino group.

The photoaffinity analogues of ubiquinone 2,3-dimethoxy-5-methyl-6-[2-[1-oxo-3-(4-azido-2-nitroanilino) propoxy]-3-methylbutyl]-1,4-benzoquinone (2'-ANAP-Q-1) and 2,3-dimethoxy-5-methyl-6-[3-[1-oxo-3-(4-azido-2-nitroanilino) propoxy]-3-methylbutyl]-1,4-benzoquinone (3'-ANAP-Q-1) have been synthesized. The required intermediate alcohols 2,3-dimethoxy-5-methyl-6-(2-hydroxy-3-methylbutyl)-1,4-benzoquinone and 2,3-dimethoxy-5-methyl-6-(3-hydroxy-3-methylbutyl)-1,4-benzoquinone were prepared in good yield from ubiquinone 1 by hydration of the side-chain double bond via hydroboration or acid catalysis, respectively. These alcohols were then coupled with 3-(4-azido-2-nitroanilino)propanoic acid, with p-toluenesulfonyl chloride in dry pyridine, to give 2'- and 3'-ANAP-Q-1. The synthetic methods presented should be of general utility in the preparation of derivatives of ubiquinone in which a reactive or reporter group is relatively close to the ubiquinone ring. By use of membrane vesicles prepared from a ubi-men-strain of Escherichia coli described previously [Wallace, B., & Young, I. G. (1977) Biochim. Biophys. Acta 461, 84-100], it has been shown that 2'- and 3'-ANAP-Q-1 substitute for ubiquinone 8 in the NADH, succinate, and D-lactate oxidase systems. Thus, these compounds may be of value in labeling respiratory chain proteins that interact with ubiquinone.

Affinity Labels↗
PubMed1984

Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli.

The structural gene for the respiratory D-lactate dehydrogenase of Escherichia coli, a membrane-bound flavoenzyme, has been subcloned from a 7 X 10(3)-base-pair chromosomal HindIII fragment containing the gene [Young, I. G., Jaworowski, A., and Poulis, M. (1982) Biochemistry 21, 2092-2095]. The complete nucleotide sequence of the 2340-base-pair PstI-SmaI subclone has been determined on both strands by the dideoxy chain termination method. A single large open reading frame is present in the nucleotide sequence. The reading frame is preceded by a good ribosome binding site and numerous possible promoter sequences, and is followed by a typical rho-independent termination sequence. The reading frame predicts that the D-lactate dehydrogenase polypeptide consists of 571 amino acids (including the initiating methionine residue) with Mr = 64613. The protein does not have a low overall polarity, nor does it contain unusually hydrophobic stretches. It appears to contain a short repeat which is homologous with the well characterized L-lactate dehydrogenases in the vicinity of the 'essential' cysteine residue. Apart from this, homology with other proteins of known sequence has not been detected.

Amino Acid Sequence↗
PubMed1981

Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon.

The nucleotide sequence of the structural gene coding for the respiratory NADH dehydrogenase of Escherichia coli has been determined by the chain-termination method. The reading frame for the protein starts with the unusual initiation codon UUG and predicts an amino acid sequence of 434 residues (Mr = 47 304). The reading frame was confirmed by protein chemical studies including determination of the N-terminal sequence of the protein. The product made in vivo was found to have threonine as its N-terminal residue, indicating that the initiating N-formylmethionine had been removed by post-translational processing.

Amino Acid Sequence↗