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Biomedical subjects

B López

Publications and source records attributed to B López.

50 records · Page 3Linked to original sources
PubMed1992

The administration of foot and mouth disease vaccine with oil adjuvant and its influence on the diagnosis of bovine tuberculosis.

The possible influence of vaccination with oil adjuvanted foot and mouth disease vaccines on the tuberculin response was investigated in 32 normal guinea pigs and 190 non-tuberculous bovines. Circulating anti-Mycobacterium bovis IgG antibodies were analysed by an enzyme-linked immunosorbent assay (ELISA), in order to determine the effect of the vaccination on the humoral response against mycobacteria in cattle. Control animals were either nonvaccinated or injected with aluminium hydroxide adjuvanted vaccine. Administration of foot and mouth disease vaccine had no apparent influence on the tuberculin responses of either guinea pigs or cattle, nor did it influence the level of anti-M. bovis antibodies in cattle.

Adjuvants, Immunologic↗
PubMed1991

Echocardiographic assessment of doxazosin on left ventricular mass in patients with essential hypertension.

A single daily dose of doxazosin taken during a 12-week period produced a significant reduction in blood pressure and left ventricular mass index in patients with mild or moderate hypertension. The systolic shortening coefficient was also increased and a trend in the improvement of ejection fraction, rate of circumferential fiber shortening, systolic contraction time, and preejective/ejective ratio was observed. No change in heart rate was recorded and no patients had side effects. The serum lipid profile was modified favorably, particularly with regard to the low-density lipoprotein cholesterol/high-density lipoprotein cholesterol ratio. By producing a reduction in blood pressure and left ventricular mass while favorably modifying the serum lipid profile, doxazosin produced a beneficial change in the overall coronary heart disease risk profile.

Antihypertensive Agents↗
PubMed1991

Reciprocal cellular and humoral immune responses in bovine tuberculosis.

A sandwich ELISA for the detection of gamma interferon showed higher sensitivity and specificity than an indirect ELISA for mycobacterial antibodies in the diagnosis of bovine tuberculosis. Circumstantial evidence of an inverse relationship between cellular and humoral immune responses to Mycobacterium bovis was found in cattle with natural infection.

Animals↗
PubMed1991

[Shortened preoperative preparation in diffuse hyperthyroid goiter: experience in 34 patients].

Conventional preparation of goitrous hyperthyroid patients using lugol and propranolol may take 2 weeks. This period may be shortened using sodium iopodate and dexamethasone. We used 500 mg of sodium iopodate and 1 mg dexamethasone for 4 days in 34 hyperthyroid patients. Surgical indication derived from failure to medical treatment (68%), large goiter (27%) or adverse reaction to PTU (6%). Clinical euthyroidism was achieved after 4 days in all patients. T3 levels decreased from 482 +/- 26.2 to 137.6 +/- 3.7 ng/dl and T4 from 20.6 +/- 1.04 to 15.2 +/- 0.5 micrograms/dl (p < 0.005). Surgery was uneventful in 33 patients, one subject developed supraventricular tachycardia responsive to verapamil. Electron microscopy of the removed thyroid tissue revealed marked decrease of superficial villi and large phagosomes. Thus, sodium iopodate and dexamethasone are effective and safe for preoperative preparation of hyperthyroid patients.

Adolescent↗
PubMed1990

Further evaluation of an indirect enzyme-linked immunosorbent assay for the diagnosis of bovine tuberculosis.

The sensitivity and specificity of an ELISA for the detection of bovine IgG anti-Mycobacterium bovis antibodies were 73.6% and 94.1%, respectively, as determined in 53 bacteriologically confirmed tuberculous cattle and 101 healthy cattle from a tuberculosis-free area. In addition, the results of ELISA and tuberculin tests in 149 cattle were compared with those of subsequent necropsy studies. Both tests failed to detect 2 animals with tuberculous lesions and positive culture; 3/12 cattle with M. bovis isolation and no lesions, and 2/7 with atypical mycobacterial infection reacted to tuberculin, but none had antibodies; in 128 cattle with neither lesions nor mycobacterial isolation, 6 were tuberculin reactors and 7 others had antibodies. Negative results were obtained by ELISA in 21/22 paratuberculous cattle. Antibodies were not detected in 88.9% to 96.4% of 697 cattle from two tuberculin negative herds of an endemic area. In a herd with proved M. bovis infection, distribution of seropositive animals in tuberculin and non-tuberculin reactors was similar. Antibody responses to cutaneous tuberculin stimuli were observed in 4 experimentally infected cattle, but only in 2/10 healthy controls after repeated PPD stimuli. Nine controls which had either received a single tuberculin dose or none showed no increase in antibody levels. The low sensitivity of this ELISA limits its usefulness as a diagnostic tool for bovine tuberculosis eradication campaigns. However, it could be helpful in epidemiological surveillance if its efficiency to identify infected herds is demonstrated.

Animals↗
PubMed1989

[Pathologic fracture of the femur due to primary hyperparathyroidism].

Pathologic fractures are not commonly described in primary hyperparathyroidism. A 31 year old male with primary hyperparathyroidism developed severe hypercalcemia and cystic osteitis. Bone pains and a fracture of the femur followed. A parathyroid adenoma was demonstrated by ultrasonography. After resection of the tumor, a normalization of serum Ca levels and clinical recovery took place.

Adenoma↗
PubMedDate not supplied

[The Mycobacteria Growth Indicator Tube (MGIT) is useful for anticipating the resistance of Mycobacterium tuberculosis to rifampicin but not to isoniazid].

A previously proposed MGIT protocol was assessed focussing on its reliability to test the mainstay antituberculous drugs. Isoniazid (H) (0.1 mg/l) and rifampin (R) (1 mg/l) were assayed against 109 Mycobacterium tuberculosis isolates affecting patients at high risk of multidrug-resistant tuberculosis. All isolates were simultaneously tested on Löwenstein-Jensen medium by the proportion method considered the gold standard. MGIT readings were accomplished within 2 and 22 days after inoculation, at day 10, 93.4% of the tests were completed. Unsatisfactory to evaluate H activity, the assay misclassified 8.8% (5/57) H susceptible and 7.7% (4/52) H resistant isolates. Otherwise, it yielded correct results for all 60 R susceptible isolates and 93.9% (46/49) R resistant isolates. Properly backed-up by a conventional test and targeting high risk patients, the MGIT system proved to be a useful aid to anticipate most of resistance to R. Accuracy, cost and turnaround time were competitive compared with those of semi-automated culture-based or molecular methods.

Antitubercular Agents↗
PubMedDate not supplied

[Study of Mycobacterium bovis in milk using bacteriological methods and the polymerase chain reaction].

The frequency of Mycobacterium bovis detection in milk samples obtained from infected animals was explored in an intensive dairy area in Argentina. To this end, an "in house" polymerase chain reaction (PCR) method was developed using Mycobacterium tuberculosis Complex specific INS1-INS2 primers, and its performance was compared with that of bacteriological methods. The decontamination procedures previous to culture reduced M. bovis viability. The pathogen was identified in milk samples from 1 of 143 infected cows and in none of 43 uninfected ones. Even though PCR sensitivity was found to be 2-20 times higher than that of bacteriology in experimentally inoculated milk samples, all 186 field samples resulted negative by PCR, including the bacteriologically-confirmed one. In spite of the high prevalence of bovine tuberculosis in Argentinian dairy herds, the detection of M. bovis in milk is an unusual finding.

Animals↗
PubMedDate not supplied

[False-positive cultures due to cross contamination in tuberculosis laboratories].

Fifteen episodes of Mycobacterium tuberculosis laboratory cross-contamination suspected between 1996 and 2001 at 6 laboratories in Buenos Aires City and suburbs were investigated by IS6110 RFLP. Thirteen episodes were confirmed. Even though BACTEC 460 produced the highest number of confirmed episodes in a single laboratory, the most extended one occurred while employing conventional culture procedures in solid medium. The double repetitive element-polymerase chain reaction (DRE-PCR) was applied to 8 of these episodes and produced concordant results with those of the RFLP. The DRE-PCR appears to be a valuable tool for the prompt identification of false positive cultures. The timely rectification of defects in laboratory protocols can avert false diagnoses of tuberculosis and unnecessary prolonged treatments.

Aerosols↗
PubMedDate not supplied

[Seroepidemiological study of human hydatidosis, by means of an indirect hemagglutination test in Rio Negro County, X Region, Chile, 1989-1990].

Rio Negro Country (40 degrees 48'-40 degrees 50' South lat.; 73 degrees 12'-73 degrees 13' West long.), is located in the province of Osorno, South of Chile. It has a population of 18,031 inhabitants, with 39% in rural areas. The basic economic activity is cattle raising (cows, pigs). According to reports, prevalence of human hydatidosis in 1988 was 11.1 per 100,000. In order to contribute to a better knowledge of human hydatidosis in Rio Negro County, an indirect hemagglutination test (IHAT) was performed to 509 randomized persons. Five (1.0%) individuals resulted positive.

Adolescent↗