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Biomedical subjects

B Lamy

Publications and source records attributed to B Lamy.

At least 37 records · Page 2Linked to original sources
PubMed1991

[A prospective study of 180 percutaneous catheterizations of the axillary vein during resuscitation].

Central venous catheterization via the axillary vein was performed in 180 comatose patients with a success rate of 91%. The scarcity of serious nervous (none), thrombotic (6%), hemorrhagic (9.4%), infectious (1.8%), or thoracic (none) complications, the preservation of cerebral venous drainage, a longer distance between puncture site and tracheostomy or intubation tube make this safe and easy procedure perfectly convenient for comatose patients.

Adolescent↗
PubMed1990

[Qualitative approach of DNA content analysis by flow cytometry].

One step DNA staining procedures are very easy to use. Reduced staining time and small amount of needed components explain the world wide use of these protocols. The heterogeneity of the results obtained with these technics during a study of different mice organs has let the authors to think that there was a close relationship between this heterogeneity and DNA staining technics. The authors have observed the same discordances with some human tissues (parathyroid, prostatic adenoma...). They disappear when the same cells are stained with a procedure that associates to propidium iodide and RNAse a pretreatment of nuclei by trypsin and spermin. These molecules are DNA stabilizing components. So one can develop the hypothesis that the abnormalities observed with the one step procedures are due to a decondensated state of DNA that allows the fixation of a higher amount of stain. The main point will be to find a direct relationship with different physiological or pathological states. Now, it is necessary to sensibilise users against the risk of false abnormal DNA content detection through the one step procedures.

Animals↗
PubMed1990

[Neurogenic pulmonary edema, complication of meningeal hemorrhage: report of 4 cases].

Neurogenic pulmonary edema (NPE) observed in 4 patients admitted in Neurosurgical Intensive Care au SAH by ruptured a vascular malformation. This complication is unusual (1.9%) and has been observed in comatose patients. For 3 patients, NEP resorption was rapid, from 12 to 72 hours with a treatment by CCPV with a P.E.E.P. and with restoring the hemodynamical parameter. The drug must be discussed according to eventual deleterous side effects on cardiac output and systemic resistances. The early hemodynamical study argues for an essentially hemodynamical mechanism due to the brutal symphatic discharge created by cerebral lesions and increasing. ICP, more than a toxic lesionnal edema, as the Weidner's study shows it in ultrastructural analysis of sheep lungs.

Adolescent↗
PubMed1990

[Blood coagulation disorders in intracerebral hematoma caused by rupture of intracranial angioma. Incidences on hemorrhagic recurrence].

In 34 patients admitted in Neurological Intensive Care Unit, for a cerebral hematoma by ruptured arterio-venous malformation, are present in 26 patients, on the first day. In many cases, the APTT is increased and this can be respected. These disorders do not seem to modify the incidence of delayed hemorrhage, except for the severe perturbances, related to a DIC or an hepatic deficiency. In these cases, the delayed hemorrhage must be prevented by transfusions of platelets and/or freeze fresh plasma.

Adolescent↗
PubMed1990

[Intracerebral hematoma caused by rupture of intracranial angioma. Analysis, prognosis and clinical course of 35 comatose patients admitted to intensive care units].

The outcome of 35 patients in deep coma (GSS less than 7) due to an intracerebral hematoma following ruptured arteriovenous malformation (AVM) and admitted to a neurosurgical intensive care unit is reviewed. Eighty percent of these patients had a cerebral AVM and 20% had an AVM in the posterior fossa. All had a medical treatment of increase intracranial pressure (ICP). Twenty-four patients or 68.6% had an early surgical treatment or some days further and 13 had a good outcome, six had a persistent coma or a residual neurological deficit and five died. Four patients or 11.4% had an endovascular embolisation therapy and three had no satisfactory results and one died; all had a new intracranial hemorrhage with hematoma. Seven patients were not treated: four died before 48 hours of hospitalisation, one is in persistent coma and two are waiting an endovascular embolisation, alive but with a neurological deficit. The results suggest that neurosurgical treatment are a safe and effective means for treatment of these AVM with compressive intracranial hematoma.

Adolescent↗
PubMed1988

[Uses of flow cytometry in hematology].

Flow Cytometry, an analytical cytology technic which allows several parameters assessment in thousands cells per seconds is frequently used in hematology. The oldest application is the leucocyte count but several news applications have been developed for the analysis of cytomorphological and functional parameters of normal cells and for detection, classification of tumor cells. Most frequent applications are: The phenotyping of cells and the evaluation of relation between antigens and pathology. The cell cycle analysis which can be different in leukemic cells or which can be modified with chemotherapy or other agents. (Radiotherapy ...) Flow Karyotyping and in situ hybridization will extend applications of Flow Cytometry in hematology.

Blood Cells↗
PubMed1988

A liposomal contrast agent. Preliminary communication.

Iopamidol-carrying liposomes were studied as potential hepatosplenographic contrast agents. Large unilamellar vesicles (0.3-1 mu) prepared from phosphatidylcholine:Dipalmotylphosphatidic acid (PC:DPPA) 9:1 and 300 MgI/mL iopamidol solution showed favorable entrapment measured as mg entrapped iodine/mg lipids (I/L). The effect of extrusion through polycarbonate membranes on liposome characteristics and in vivo distribution was investigated. Extrusion above the transition temperature of lipids reduced the average size and size distribution and increased the I/L ratio. Distribution studies of extruded and nonextruded liposomes in rats demonstrated different behavior of the preparations; extruded liposomes showed higher spleen uptake than did unextruded, while liver uptake was comparable; lung entrapment, observed with unextruded particles, was almost eliminated with extruded liposomes. Preliminary imaging studies in rats were carried out at a dose of 250 mgI/kg; typical computed tomography (CT) scans of the liver demonstrated contrast enhancement of greater than or equal to 60 HU from 90' up to 240' after injection.

Animals↗
PubMed1988

[Flow cytometry in the detection of residual disease].

Flow cytometry is a biological analysis technics whose applications may be used for minimal residual disease evaluation. From these applications we retained the DNA content measurement (with DNA index), surface markers analysis, and flow karyotyping. The threshold cell aneuploidy detection depend of the DNA index of leukemia cell population. The establishment of a relation between cell surface markers and malignant character of the cell is difficult to obtain. Study of CALLA antigen in leukemic patients have shown variations of antigenic density with different categories of studied patients. Malignant cells studied at the diagnosis or at the relapse of leukemia have a mean antigenic density higher than normal cells. This fact could be used to evaluate minimal residual disease as shown with recent clinical observations.

Antigens, Neoplasm↗
PubMed1988

[Culture of leukemic progenitor cells in acute non-T lymphoid leukemia].

A preliminary study of the culture of leukemic progenitor cells (CFU-L) from acute non-T lymphoblastic leukemia was undertaken for 25 patients (19 were considered in complete remission and 6 in the acute phase of the disease). Two culture systems were tested; a double layer agar-liquid phase and a single layer of methylcellulose. The major problem was the characterization of the colonies: immunological labelling coupled with cytofluorometry, as well as cytomorphology, cytogenetic and more recently molecular biology may allow the characterization of the CFU-L. The culture of CFU-L appears to be an efficient method for detecting residual leukemic cells which can be used to evaluate the quality of both the remission obtained and that of autologous bone marrow after purging.

Agar↗
PubMed1987

Preclinical studies of a panel of 12 monoclonal antibodies in view of bone marrow purging in acute lymphoblastic leukemia.

We analysed the optimal conditions for autologous bone marrow purging using complement binding monoclonal antibodies (mAbs). Twelve mAbs belonging to four clusters (CD9, CD10, CD19, CD24), alone or combined were evaluated by using direct cytotoxicity and clonogenic assays. We observed the following data: (1) optimal cytotoxicity was reached with doses of 1-10 micrograms mAbs for 10(7) cells, (2) the concentration of the cell suspension had to be below 3 X 10(7)/ml, (3) combinations of mAbs were more effective than a single mAb treatment, (4) in the case of an IgM isotype, there seems to be a clear dissociation between the amount needed for optimal toxicity and that for antigenic saturation measured by cytofluorometry.

Antibodies, Monoclonal↗
PubMed1986

[Brain toxoplasmosis: from a case disclosing AIDS. Recall of diagnostic, developmental and therapeutic elements].

The authors are studying from a personal case revealing an AIDS, and from bibliographical cases, the treated evolution of toxoplasmic encephalitis in immuno-depressed patients and the elements of the diagnosis. They are insisting on the interest of the brain biopsy, and on the importance of the inflammatory brain oedema. The therapy consists on sulfamides and anti-oedematous drugs. They are thinking that the corticosteroids must be avoided. The evolution of cerebral lesions, seen on the repeated computed tomographies is often good but the prognosis remains bad according to the immuno-deficiency.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Removal of marrow T cells with OKT3-OKT11 monoclonal antibodies and complement to prevent acute graft-versus-host disease. A pilot study in ten patients.

We studied the feasibility of T cell depletion of bone marrow for transplantation in preventing acute graft-versus-host disease GVHD in patients having a high risk of acute GVHD. We report our preliminary clinical experience of the ex-vivo treatment of allogeneic marrow using a pan-T monoclonal antibody combination (OKT3-OKT11) plus baby rabbit complement. Ten patients received allografts from HLA-identical sibling donors. All patients had malignant hematological disease with poor prognosis (6 acute leukemia, 3 chronic granulocytic leukemia, and 1 multiple myeloma). Nine patients were at high risk of acute GVHD (older than 30 years for 4 patients, chronic granulocytic leukemia in acute or accelerated phase for 3, and acute leukemia in relapse for 2). Posttransplant management with methotrexate was maintained until day 100 in the first four patients and stopped at day 11 for the six others. The ex-vivo treatment with the OKT combination and complement removed 88.3% +/- 11.8 of the T lymphocytes. The myeloid progenitors recovered at the end of the procedure showed a moderate effect of monoclonal antibodies and complement (75.8% +/- 12.2). Engraftment was achieved in all patients: 23.3 days +/- 5.10 to reach 0.5 X 10(9) granulocytes per liter and 31.5 days +/- 12.2 to reach 50 X 10(9) platelets per liter. No acute GVHD was observed in this group of patients. Of the 10 patients, 7 are alive and well and have been in continuous remission from 2-10 months. Three patients have relapsed.

Adolescent↗