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B Lenkiewicz

Publications and source records attributed to B Lenkiewicz.

15 recordsLinked to original sources
PubMed1991

[Evaluation of the test using polybrene for the detection of anti-erythrocyte antibodies].

The polybren test (P) was used parallelly with the antiglobulin test (PTA-LISS) and enzymatic test (LEN) for detection of HIV-1 antibodies. The analysis of the results of testing of 646 sera showed that the P test is a simple method making possible detection of most antibodies of the IgG class without using antiglobulin serum within up to 3 minutes. The sensitivity of the P test was high. The sera with antibodies to the antigens of the systems Rh, Kidd, Duffy, MNSs and other less frequent antibodies such as anti-Vel, anti-Coa anti-Co(b), anti-Dia and anti-Ge showed the highest activity in the P test. In one serum anti-D antibodies, in three sera anti-C and in one serum anti-Ge antibodies were detected only in the P test, while no reactions were obtained in the PTA-LISS and LEN tests. Among the immune antibodies the exception was the antibody to Kell antigens which weakly reacted in the P test and in most cases an additional phase of testing with antiglobulin PTA-P was necessary for its demonstration. The P test was of low usefulness in the detection of cold IgM antibodies such as anti-Le, anti-Fe1, anti-I and anti-HI.

Blood Group Antigens↗
PubMed1989

Serological and immunological characteristics of maternal anti-Rh(D) antibodies in predicting the severity of haemolytic disease of the newborn.

A number of factors were analyzed for their predictive value in indicating the severity of haemolytic disease of the newborn (HDN) in 72 infants. The factors investigated were: maternal antibody titre in the indirect antiglobulin test, the number of antibody molecules on sensitized standard red cells evaluated by a radiometric antiglobulin test, the IgG subclass specificity and the reactivity in monocyte-monolayer assay (MMA) and in the rosette assays with lymphocytes and granulocytes from healthy individuals. The results of the MMA correlate much better with the severity of HDN than the antibody titre. In clinically unaffected infants the reactivity in the MMA never exceeded 20%, while in the severe/very severe group it was always greater than 20% (in 95% of very severe cases even above 50%). The number of IgG-bound molecules was also shown to closely correlate with the clinical severity and there was a much greater proportion of severe/very severe cases exhibiting combined IgG1 and IgG3 specificity. Of all the evaluations performed the rosette assays with lymphocytes and granulocytes were found to be less useful in predicting the severity of HDN.

Erythroblastosis, Fetal↗
PubMed1986

Weak A phenotypes possibly caused by mutation.

A family is described in which an apparent Ay phenotype was transmitted through 2 generations. We favor a mutation of the A allele as the most likely cause of the phenotype. Activities of the serum A-gene-specified transferase were not detected in any of the 3 family members with the Ay phenotypes.

ABO Blood-Group System↗
PubMedDate not supplied

[Intrauterine blood transfusions in the treatment of severe serologic conflict].

Efficacy of the intraperitoneal and intravenous blood transfusions guided ultrasonographically was evaluated in severe cases of fetal hemolytic disease due to Rh conflict. It was shown, that survival rate of fetuses without generalized edema is two-fold higher in the group treated with intraperitoneal blood transfusions in comparison with the control group. It was found, that the proportion of erythrocytes containing HbA in umbilical blood of newborn is related to the number of intraperitoneal transfusions. One has to underline the failure of such a treatment in features with generalized edema. In such case intravenous blood transfusion is a method of choice.

Blood Transfusion, Intrauterine↗
PubMedDate not supplied

First example of By phenotype.

In ABO blood grouping red cells of a young healthy woman behaved like O, but in her serum anti-B was lacking. By absorption-elution technique the very weak B activity was revealed. The saliva contained B and H substances, although the B to H ratio was significantly reduced, when compared to the normal B and Bm secretors. In the serum B transferase activity was not demonstrated. The presented results were comparable to those described in persons with Ay phenotype. Therefore, this weak B variant can be classified into By category.

ABO Blood-Group System↗
PubMedDate not supplied

[Prediction of the severity of fetal erythroblastosis using the erythrocyte phagocytosis test and evaluating anti-Rh antibody titers and their levels by autoanalysis].

Erythrophagocytosis++ test was performed in 45 pregnant Rh-negative women together with routine anti-Rh antibodies assay with indirect antiglobulin test and their concentration determined in an autoanalyser. It was found that the degree of ++phagocytosis of the standard erythrocytes immunized with maternal anti-Rh antibodies by the monocytes from healthy subjects is an important predictive factor enabling to foresee a severity of hemolytic disease in newborn babies. ++Phagocytosis over 50% predicts a severe course of the disease whereas under 20%--a mild form of the serologic conflict.

Autoanalysis↗
PubMedDate not supplied

[Remarks on routine use of the enzyme test LEN (LISS Enzyme) in immunohematological studies].

The authors report one-year experience with the enzymatic test LEN (LISS-Enzym) replacing the one-step papain test in the compatibility testing, and in place of the two-step papain test in the detection and identification of red cells allo-antibodies. A wide range of investigations confirmed the usefulness of this test in the detection and antibodies specificity, mainly from the Rh system which are often undetectable by means of the indirect antiglobulin tests. It is concluded that in compatibility tests the LEN test should replace the low-sensitivity one-step papain test used as yet. It is stressed that in case of positive reactions only in the LEN test suggesting presence of autoantibodies the test should be repeated bringing to 37 degrees C the reacting components before their mixing. This makes possible elimination of clinically not significant cold autoantibodies.

Diagnostic Tests, Routine↗
PubMedDate not supplied

[Anti-M antibodies in the pathogenesis of hemolytic disease of the newborn].

Two cases are presented of serological fetomaternal incompatibility in the MNSs group system diagnosed for the first time in Poland. Anti-M antibodies of the mother caused in one newborn haemolytic disease with acute anaemia requiring blood transfusions. In the other newborn clinical signs of the disease failed to appear despite a positive direct antiglobulin test. The results are presented of immunohematological studies calling attention to difficulties connected the diagnosis of haemolytic disease of the newborn caused by anti-M antibodies. This problem is discussed more extensively in the light of available literature.

Erythroblastosis, Fetal↗