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Biomedical subjects

B M Barnes

Publications and source records attributed to B M Barnes.

At least 19 recordsLinked to original sources
PubMed2004

Antifreeze proteins in Alaskan insects and spiders.

Prior to this study, antifreeze proteins (AFPs) had not been identified in terrestrial arthropods from the Arctic or anywhere in Alaska. The hemolymph of 75 species of insects and six spiders from interior and arctic Alaska were screened for thermal hysteresis (a difference between the freezing and melting points), characteristic of the presence of AFPs. Eighteen species of insects and three spiders were shown to have AFPs. Ten of the insects with AFPs were beetles including the first species from the families Chrysomelidae, Pythidae, Silphidae and Carabidae. In addition, the first Neuropteran to have AFPs was identified, the lacewing Hemerobius simulans together with the second and third Diptera (the first Tipulids) and the second and third Hemiptera, the stinkbug Elasmostethus interstinctus (the first Pentatomid), and the water strider Limnoporus dissortis (the first Gerrid). Prior to this study, 33 species of insects and three spiders had been reported to have AFPs. Most AFP-producing terrestrial arthropods are freeze avoiding, and the AFPs function to prevent freezing. However, some of the AFP- producing insects identified in this study are known to be freeze tolerant (able to survive freezing) to very low temperatures (-40 to -70 degrees C).

Acclimatization↗
PubMed2002

Body temperature patterns before, during, and after semi-natural hibernation in the European ground squirrel.

Ground squirrels undergo extreme body temperature fluctuations during hibernation. The effect of low body temperatures on the mammalian circadian system is still under debate. Using implanted temperature loggers, we recorded body temperature patterns in European ground squirrels kept in an enclosure under natural conditions. Although hibernation onset was delayed, hibernation end corresponded closely to that measured in a field population. Circadian body temperature fluctuations were not detected during deep torpor, but indications of circadian timing of arousal episodes at higher temperatures were found at the beginning and end of hibernation. One male exhibited synchronised arousals to a relatively constant phase of the day throughout hibernation. All animals first entered torpor in the afternoon. Daily body temperature fluctuations were decreased or distorted during the first days after hibernation. We hypothesise that hibernation may affect the circadian system by either decreasing the expression of the circadian oscillator, or by decreasing the amplitude of the circadian oscillator itself. possibly due to gradual, temperature dependent, internal desynchronisation. The latter mechanism may be beneficial because it might facilitate post-hibernation re-entrainment rates.

Animals↗
PubMed2000

mRNA stability and polysome loss in hibernating Arctic ground squirrels (Spermophilus parryii).

All small mammalian hibernators periodically rewarm from torpor to high, euthermic body temperatures for brief intervals throughout the hibernating season. The functional significance of these arousal episodes is unknown, but one suggestion is that rewarming may be related to replacement of gene products lost during torpor due to degradation of mRNA. To assess the stability of mRNA as a function of the hibernation state, we examined the poly(A) tail lengths of liver mRNA from arctic ground squirrels sacrificed during four hibernation states (early and late during a torpor bout and early and late following arousal from torpor) and from active ground squirrels sacrificed in the summer. Poly(A) tail lengths were not altered during torpor, suggesting either that mRNA is stabilized or that transcription continues during torpor. In mRNA isolated from torpid ground squirrels, we observed a pattern of 12 poly(A) residues at greater densities approximately every 27 nucleotides along the poly(A) tail, which is a pattern consistent with binding of poly(A)-binding protein. The intensity of this pattern was significantly reduced following arousal from torpor and undetectable in mRNA obtained from summer ground squirrels. Analyses of polysome profiles revealed a significant reduction in polyribosomes in torpid animals, indicating that translation is depressed during torpor.

Animals↗
PubMed2000

Effects of ambient temperature on metabolic rate, respiratory quotient, and torpor in an arctic hibernator.

Arctic ground squirrels (Spermophilus parryii) overwinter in hibernaculum conditions that are substantially below freezing. During torpor, captive arctic ground squirrels displayed ambient temperature (T(a))-dependent patterns of core body temperature (T(b)), metabolic rate (TMR), and metabolic fuel use, as determined by respiratory quotient (RQ). At T(a) 0 to -16 degrees C, T(b) remained relatively constant, and TMR rose proportionally with the expanding gradient between T(b) and T(a), increasing >15-fold from a minimum of 0.0115 +/- 0.0012 ml O(2). g(-1). h(-1). At T(a) 0-20 degrees C, T(b) increased with T(a); however, TMR did not change significantly from T(b) 0 to 12 degrees C, indicating temperature-independent inhibition of metabolic rate. The overall change in TMR from T(b) 4 to 20 degrees equates to a Q(10) of 2.4, but within this range of T(b), Q(10) changed from 1.0 to 14.1. During steady-state torpor at T(a) 4 and 8 degrees C, RQ averaged 0.70 +/- 0.013, indicating exclusive lipid catabolism. At T(a) -16 and 20 degrees C, RQ increased significantly to >0.85, consistent with recruitment of nonlipid fuels. RQ was negatively correlated with maximum torpor bout length. For T(a) values <0 degrees C, this relationship supports the hypothesis that availability of nonlipid metabolic fuels limits torpor duration in hibernating mammals; for T(a) values >0 degrees C, hypotheses linked to body temperature are supported. Because anterior body temperatures differ from core, overall, the duration torpor can be extended in hibernating mammals may be dependent on brain temperature.

Animals↗
PubMed1998

Differential regulation of uncoupling protein gene homologues in multiple tissues of hibernating ground squirrels.

Nonshivering thermogenesis in brown adipose tissue (BAT) provides heat through activation of a mitochondrial uncoupling protein (UCP1), which causes futile electron transport cycles without the production of ATP. Recent discovery of two molecular homologues, UCP2, expressed in multiple tissues, and UCP3, expressed in muscle, has resulted in investigation of their roles in thermoregulatory physiology and energy balance. To determine the expression pattern of Ucp homologues in hibernating mammals, we compared relative mRNA levels of Ucp1, -2, and -3 in BAT, white adipose tissue (WAT), and skeletal muscle of arctic ground squirrels (Spermophilus parryii) hibernating at different ambient and body temperatures, with levels determined in tissues from ground squirrels not in hibernation. Here we report significant increases in mRNA levels for Ucp2 in WAT (1. 6-fold) and Ucp3 in skeletal muscle (3-fold) during hibernation. These results indicate the potential for a role of UCP2 and UCP3 in thermal homeostasis during hibernation and indicate that parallel mechanisms and multiple tissues could be important for nonshivering thermoregulation in mammals.

Adipose Tissue↗
PubMed1997

Leptin prevents posthibernation weight gain but does not reduce energy expenditure in arctic ground squirrels.

In mammals, leptin reduces energy intake and may increase energy expenditure as a means to maintain body weight and/or adiposity at an appropriate level. Hibernating mammals seasonally alter body mass, food intake, and body composition and, therefore, represent an attractive model for investigating the physiological regulation of changing body mass and adiposity. Previous experiments in our laboratory demonstrated that administration of mouse recombinant leptin reduces food intake and body weight in arctic ground squirrels during prehibernation fattening. In addition, leptin appeared to reduce metabolic efficiency (weight gain per unit of energy intake). This result suggests that reduced food intake alone may not account for the observed weight loss. Here, we describe the effect of a 3-week constant infusion of leptin given to posthibernation arctic ground squirrels on food consumption and energy expenditure. Mouse recombinant leptin (1 mg/ml) was administered through subcutaneously implanted mini-osmotic pumps (10 microliters/hr flow rate). Resting metabolic rate was monitored before and during the 3-week leptin administration period by indirect calorimetry. Body temperature and locomotory activity were monitored continuously by abdominal radiotransmitters. At the end of the leptin administration period, thermogenic capacity was evaluated by measuring brown fat uncoupling protein-1 mRNA and protein levels. Leptin administration resulted in reduced food intake and prevented posthibernation weight gain, but it did not alter any of the measured parameters of energy expenditure.

Adipose Tissue↗
PubMed1991

Warming up for sleep? Ground squirrels sleep during arousals from hibernation.

Hypothermia during mammalian hibernation is periodically interrupted by arousals to euthermy, the function of which is unknown. We report that arctic ground squirrels (Spermophilus parryii) consistently sleep during these arousals, and that their EEG shows the decrease in slow wave activity (delta power) that is characteristic of a declining requirement for sleep. These results are consistent with the novel hypothesis that the need for sleep slowly accumulates during torpor, and that returning to euthermy is periodically required to allow sleep. Sleep thus seems to be energetically expensive for a hibernating mammal, and cannot be considered solely a strategy for saving energy.

Animals↗
PubMed1990

Effect of winter high temperatures on reproduction and circannual rhythms in hibernating ground squirrels.

We tested whether prevention of hibernation in ground squirrels by midwinter exposure to high ambient temperatures influenced timing of the spring phase of reproductive maturation and the phase and period of subsequent circannual rhythms of reproduction and body mass. Exposing hibernating adult male Spermophilus lateralis to 30 degrees C for 6 weeks beginning December 4 advanced the timing of testicular recrudescence by 4-5 weeks, compared to controls left at 4 degrees C. Males exposed to 30 degrees C for 6 weeks beginning at the average time of spontaneous end of hibernation (January 15) reached reproductive maturation at a time intermediate to those of controls and of the December 4 experimental group. However, neither the date of the subsequent fall's body mass peak, the date of the next year's reproductive maturation, nor the periods of circannual rhythms of body mass and reproduction differed among groups. Premature interruption of hibernation appears to allow early expression of reproduction, but does not affect the underlying timing mechanism.

Animals↗
PubMed1989

Freeze avoidance in a mammal: body temperatures below 0 degree C in an Arctic hibernator.

Hibernating arctic ground squirrels, Spermophilus parryii, were able to adopt and spontaneously arouse from core body temperatures as low as -2.9 degrees C without freezing. Abdominal body temperatures of ground squirrels hibernating in outdoor burrows were recorded with temperature-sensitive radiotransmitter implants. Body temperatures and soil temperatures at hibernaculum depth reached average minima during February of -1.9 degrees and -6 degrees C, respectively. Laboratory-housed ground squirrels hibernating in ambient temperatures of -4.3 degrees C maintained above 0 degree C thoracic temperatures but decreased colonic temperatures to as low as -1.3 degrees C. Plasma sampled from animals with below 0 degree C body temperatures had normal solute concentrations and showed no evidence of containing antifreeze molecules.

Animals↗
PubMed1989

Suprachiasmatic nuclei influence torpor and circadian temperature rhythms in hamsters.

Siberian hamsters were maintained in a short-day photoperiod (8 h light-day) at 15 degrees C; body temperature (Tb) and locomotor activity were telemetrically recorded at 10-min intervals over the course of 5 mo. Animals manifesting repeated torpor bouts (Tb less than 30 degrees C for several hours) were subjected to lesions of the suprachiasmatic nuclei (SCN), pinealectomy, or sham operations. In the 15 wk after surgery, none of the animals with bilateral lesions of the SCN exhibited torpor; circadian Tb and locomotor activity rhythms, as determined by cosinor and power spectral analysis, also were absent in SCN-lesioned hamsters. Pinealectomized animals and brain-lesioned hamsters with intact SCN had normal circadian temperature and activity rhythms and showed torpor for at least 4 wk postsurgically. Expression of torpor and circadian rhythms of Tb and activity are dependent on intact SCN and persist for several weeks in the absence of pineal secretory activity.

Animals↗
PubMed1988

Plasma androgen and gonadotropin levels during hibernation and testicular maturation in golden-mantled ground squirrels.

The 4-5-mo hibernation season of golden-mantled ground squirrels consists of extended torpor bouts interspersed with brief, periodic intervals of normothermic arousal. Plasma levels of testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) and degree of scrotal pigmentation were measured in torpid and aroused male ground squirrels throughout a season of hibernation and in active animals after the termination of torpor. T was basal in torpid animals; beginning 3 weeks before torpor ended, T was elevated in normothermic males during the first half of periodic arousals but returned to basal levels before animals reentered torpor. After the last (terminal) arousal from torpor, T levels were moderately elevated for 4 wk and maximal for the next 6 wk before they returned to basal values. LH patterns were similar to those of T; however, levels of T and LH were positively correlated only in aroused or posthibernation males. FSH levels remained constant and low during most of the heterothermic season but increased in several torpid males within 3 days of terminal arousal. FSH levels peaked 2 wk after the end of heterothermy. Scrotal pigmentation developed over the first 4 wk after terminal arousal. Maturation of reproductive function occurs during the 4 wk after termination of heterothermy, but elevated levels of T during arousals and variable levels of FSH in the last days of torpor suggest that activation or increased sensitivity of the hypothalamic-pituitary-gonadal axis is important in the termination of heterothermy in ground squirrels.

Androgens↗
PubMed1986

Annual cycles of gonadotropins and androgens in the hibernating golden-mantled ground squirrel.

Ground squirrels, captured in the field, were housed at ambient temperatures of 23 degrees (photoperiod = 10L:14D) for 13 months. Plasma was sampled at 3 to 4-week intervals and measured for gonadotropin and androgen levels. Testis size was examined monthly by laparotomy. Male ground squirrels showed clear circannual cycles in body mass, testis size, and levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), and dihydrotestosterone (DHT). During summer and fall, FSH, LH and androgen levels were low, testes were undeveloped, and body mass was increasing. Testes began to rapidly enlarge in January and reached maximum size in February. A rise in FSH preceded gonadal growth but LH remained low until near the time of testis mass peak. LH remained elevated during spring while FSH levels fell and testes regressed. Plasma T and DHT levels generally paralleled LH concentrations; DHT levels were approximately one-fifth those of T levels. During winter animals lost weight but were only occasionally found in a slightly hypothermic condition. Females had elevated plasma LH levels (greater than 1 ng/ml) predominantly in the spring but displayed no cycle in plasma FSH levels. A second group of males held at 4 degrees for 8 months (photoperiod = stimulated natural for 47 degrees N) were regularly torpid during a hibernation season that lasted between November and May. Most (15/21) of these males did not show gonadal development by spring; these non-reproductive males had had restricted body mass gains the previous fall. Plasma FSH was low in both reproductive and non-reproductive males during fall and winter but increased in March while animals were still hibernating. FSH levels continued to increase in April only in reproductive males and reached maximal levels after hibernation was spontaneously terminated. LH titers were elevated in individual males in winter during torpor and were greater in reproductive than in non-reproductive males in May. Androgen levels were undetectable in torpid squirrels, elevated in animals sampled during periodic arousals, and elevated in most males within 3 weeks after terminating hibernation.

Androgens↗
PubMed1986

Fat ablation and food restriction influence reproductive development and hibernation in ground squirrels.

The role of white adipose tissue in development of the reproductive apparatus of male golden-mantled ground squirrels was assessed by surgical removal of fat (lipectomy) immediately prior to onset of hibernation or by manipulation of fat levels through food restriction for 5 wk preceding hibernation. Animals then were maintained without food at 6 degrees C, and they hibernated from November 1983 until April 1984. At that time, blood plasma was assayed for hormone levels, and body mass, body composition, and masses of the testes and seminal vesicle-prostate complex were determined. At autopsy, testes and sexual accessory organs were heavier in Control squirrels than in Food-Restricted or Lipectomized (LIPX) animals. Paired-testes mass was positively correlated with body mass. Testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) levels were uniformly low in animals sampled during torpor, but were elevated in 2 animals that had regained the euthermic state. The Food-Restricted animals spent more time in torpor than did the other two groups, and the percentage of decrease in body mass over the hibernation season was less for Food-Restricted than for LIPX or Control animals. Adequate lipid stores may be essential for the normal development of the reproductive apparatus of male ground squirrels. In the field, animals with insufficient fat stores may forego reproduction and thereby conserve energy for survival.

Adipose Tissue↗
PubMed1986

The influence of hibernation on testis growth and spermatogenesis in the golden-mantled ground squirrel, Spermophilus lateralis.

Testis size and spermatogenesis were monitored serially in individual golden-mantled ground squirrels before, during, and after the hibernation season. During hibernation, animals spent 81% of days in torpor at body temperatures of 3-4 degrees C. Torpor bouts of 6 days duration were interspersed with brief arousals from torpor during which animals were normothermic. In the 5 mo between December (when animals entered hibernation) and April (when torpor was spontaneously terminated), the estimated mass of testes increased gradually from 500 to 1100 mg, but spermatogenesis did not advance beyond pachytene spermatocytes, which were present before hibernation began. In contrast, during the month after torpor was terminated, testes increased rapidly to 3500 mg and after 31 days, spermatozoa were found in the epididymides. We suggest that the limited testis growth that occurred during the hibernation season was restricted to intervals during which squirrels were aroused from torpor. The major portion of gonadal growth and spermatogenesis in the laboratory, and presumably in the field, occurs after ground squirrels have regained the normothermic state. Since males are reproductively mature when first trapped in spring, these findings suggest that males are normothermic for several weeks before they emerge from their hibernacula in the spring.

Aging↗
PubMed1984

Environmental and endogenous control of reproductive function in the Great Basin pocket mouse Perognathus parvus.

Pocket mice captured in the field at various times of year were introduced into laboratory experiments to examine the short-term sensitivity of reproductive function to environmental factors, principally day length, and the tendency of the reproductive system to become active spontaneously over longer durations. Spontaneous enlargement and partial activation of the gonads occurred over the course of 4-5 mo in continuous darkness during the hibernation season. Males held for 13 mo in 12L:12D showed patterns of testicular enlargement, but with only partial regression; the degree of endogeneity in the reproductive control system of P. parvus is therefore considerably below that of the pronounced and persistent "endogenous circannual rhythms" shown by certain rodents of the squirrel family. Responses to day length varied seasonally. The partially activated reproductive system of mice that emerged from hibernation in spring was further stimulated by long days (16L:8D); in summer gonadal growth was insensitive to differences in day length, and in autumn the gonads remained undeveloped in short days (8L:16D) but were sensitive to stimulation by long days. This "photoperiodic" response of P. parvus is based on an endogenous circadian rhythm of photosensitivity as proposed by Bünning (1936). We also found that reproductive function of P. parvus is somewhat retarded by low temperature and reduced availability of water. We discuss the general nature of environmental sensitivity of reproductive function and the ways in which the photoperiodic response and spontaneous pattern of winter gonadal development in P. parvus are likely to interact with environmental factors that lead to fine-tuning the final reproductive response.

Animals↗
PubMed1980

Involuntary commitment and the treatment process: a clinical perspective.

The serious question of involuntary hospitalization at this point in history is properly referred to due process in court. While numerous studies have addressed the decisions, statistics and outcomes in this area, insufficient attention has been paid to how the clinician may productively incorporate the clinical experience mobilized by the procedure into the therapeutic work. The authors suggest that the issues raised include maintenance of the alliance, responsibility, dependence, limit-setting, the subjective experience of the process, and the problem of counter-transference. They indicate how the various aspects of commitment may be explored aso as to yield maximal therapeutic benefit, and maintain the alliance during the conflicts of an adversary procedure.

Adult↗