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Biomedical subjects

B M Bas

Publications and source records attributed to B M Bas.

At least 19 recordsLinked to original sources
PubMed1998

[Comparison of two techniques of automated screening in respiratory and food allergy (UniCAP versus IMMULITE)].

Screening tests plays an essential part in primary allergy diagnosis, therefore they have to show the highest level of reliability. In order to assess how UniCAP system fulfill these requirements, in comparison with Immulite, we analysed the results obtained with Phadiatop, (UniCAP) and AlaTOP (IMMULITE) in 110 patients with a clinical positive diagnosis for inhalant allergy. Furthermore we compared the results from food allergy screening tests performed in 103 patients with a clinical positive diagnosis for food allergy and we analysed 110 tests results of controls with negative diagnosis for allergy. We obtained the following results: [table: see text] Samples for which the tests results were not in concordance with the clinical diagnosis were tested with the follow up panel of the different screening tests. We obtained a better concordance with Phadiatop, than with Alatop. Specificity of the inhalant allergy screening tests is the same in the two systems. We also obtained a better concordance with fx5 than with fp5. We conclude on the basis of their respective clinical sensitivity that both Phadiatop and fx5, available on UniCAP, better answer inhalant and food allergy screening needs than Alatop and fp5, available on Immulite.

Adolescent↗
PubMed1997

The first fully automated allergy analyser UniCAP: comparison with IMMULITE for allergy panel testing.

Automated immunoassay systems should be convenient to handle, flexible and give reliable results. To investigate the extent to which the UniCAP System met the above requirements, compared with the IMMULITE System, we compared the Phadiatop (UniCAP) and AlaTOP (IMMULITE) results of 110 patients with positive clinical diagnoses for inhalant allergy. In addition, we compared food screening test results of 103 patients with a clinical positive diagnosis for food, and 110 test results of controls with negative diagnosis for allergy. Phadiatop had a sensitivity of 96% and a specificity of 92%. AlaTOP had a sensitivity of 86% and a specificity of 94%. For food screening the results were: 75% sensitivity and 82% specificity for fx5 (UniCAP) and 63% sensitivity and 71% specificity for fp5 (IMMULITE). Furthermore, those samples for which the test results which were not in concordance with the clinical diagnosis were tested with the follow-up panel of the different screening tests. For the AlaTOP follow-up we had to use the DPC microplate System (Milenia), because single allergen testing is not yet possible on the IMMULITE System. With regard to sensitivity, the UniCAP specific inhalant allergen tests and the original Phadiatop results showed closer agreement with each other than did the Milenia specific allergen results with the AlaTOP. The specificity of the single inhalant allergen tests was the same for both systems. For food allergy testing the UniCAP System shows closer agreement between the screening and the follow-up results than does the IMMULITE. The hands on time for loading 44 samples was practically the same for both systems, but for the follow-up tests the Milenia System is used next to the IMMULITE. Therefore from a logistical point of view the UniCAP System is more convenient. From these results we conclude that both logistically and clinically UniCAP seems to meet our requirements better than the IMMULITE.

Adolescent↗
PubMed1994

Endotoxin binding to platelets in blood from patients with a sepsis syndrome.

Endotoxin, the lipopolysaccharide cell wall constituent of Gram-negative bacteria, produces symptoms of the Gram-negative sepsis syndrome. By measuring endotoxin in blood from septic patients it may be possible to select a subpopulation of patients in which mortality can be prevented by treatment with anti-endotoxin antibodies. We evaluated the performance of an endotoxin-free blood-collection tube. Within-run and between-run CVs of our endotoxin assay were 4-18% and 8-20%, respectively. In endotoxin-positive samples (LPS > or = 6 ng/L), the concentration of endotoxin in platelet-rich plasma was significantly higher (P < 0.001) than in platelet-poor plasma. Apparent binding of endotoxin to platelets ranged from 0% to 92%. The correlation between the apparent percentage binding of LPS to platelets and the platelet count in platelet-rich plasma is linear and positive, but LPS is not bound solely to platelets. We conclude that endotoxin must be measured in platelet-rich plasma.

Bacteremia↗
PubMed1993

A short evaluation of a new haematological analyser: the Cobas Argos 5 Diff.

In order to evaluate the Cobas Argos 5 Diff, a five-part differential haematological analyser, we performed a comparative study using our current analyser, the Toa E-5000, and the manual leukocyte differential. Samples (n = 593) were collected from various departments in our hospital and were tested on both Cobas Argos 5 Diff and Toa E-5000. Regression lines were calculated for all standard haematological quantities. A group of 100 samples, including 50 negative (absence of flags) and 50 positive (presence of flags), on the Cobas Argos 5 Diff were randomly selected from the above-mentioned collection. All these samples had been checked under the microscope by two persons counting 200 cells each. The comparison of standard haematological quantities between Cobas Argos 5 Diff and the Toa E-5000 shows good correlation: Red Blood Cells r = 0.993; White Blood Cells r = 0.998; Haemoglobin r = 0.989; Haematocrit r = 0.982; Thrombocytes r = 0.988; Lymphocytes (%) r = 0.983; Neutrophils (%) r = 0.959. The correlation between Cobas Argos 5 Diff and the manual leukocyte differential shows good correlation for lymphocytes and neutrophils, with acceptable correlation for eosinophils. The correlation for basophils and monocytes was less acceptable, especially for normal samples. We conclude that the efficiency of the Cobas Argos 5 Diff is good (93%) and that it is a suitable haematological cell analyser.

Autoanalysis↗
PubMed1989

Effect of a moderate fish intake on haemostatic parameters in healthy males.

This article describes the results of a dietary intervention study performed in three different centers. In the study the effect of a diet enriched with fish on the coagulation tendency of blood was investigated. Two groups of 40 volunteers were given a dietary supplement consisting of 135 g of canned mackerel or meat paste (control) for a 6 weeks period. Compliance, monitored by measuring the urinary excretion of lithium, added to the supplements, was about 80%. Before, during and at the end of the experimental period a number of hemostatic parameters, reflecting the coagulation tendency of blood and the procoagulant activity of monocytes, were measured. The fish supplement did not cause a significant effect on the prothrombin time and on the levels of factor VII, activated factor VII, antithrombin III, von Willebrand factor, fibrinogen, plasminogen and alpha 2-antiplasmin. A slight but transient prolongation in the activated partial thromboplastin time was observed as well as a significant increase in the factor X level, which became more pronounced with prolongation of the experimental period; no activated factor X was found. A tendency towards a stimulation of monocyte procoagulant activity was noticed.

Adult↗
PubMed1988

Analytical performance of three commercially available nephelometers compared for quantifying proteins in serum and cerebrospinal fluid.

We investigated the analytical performance and imprecision of three commercially available nephelometers for the quantification of various proteins in pooled serum and cerebrospinal fluid. Albumin, transferrin, IgG, IgA, and IgM in serum were determined nephelometrically (BNA, Behring; Array, Beckman) and turbidimetrically (Cobas Bio, Hoffmann-La Roche). All these proteins in cerebrospinal fluid except transferrin were determined nephelometrically with all three systems. CVs and lower limits of detection were compared for all instruments, and stability of calibration curves was evaluated.

Blood Proteins↗
PubMed1987

Faecal lysozyme: determination, reference intervals and some data in gastro-intestinal disease.

After diluting faecal samples with a solution of Brij and saline and subsequently ultrafiltrating the faecal mixtures, lysozyme concentration can be reproducibly measured in the obtained faecal fluids, using a turbidimetric method. Measuring faecal lysozyme concentration enables discrimination normal individuals and patients with irritable bowel syndrome between patients with inflammatory bowel disease and colonic cancer. Lysozyme distribution in stools appears to be homogeneous. Faecal lysozyme concentration is stable when samples are stored during at least 1 wk at 6 degrees C. It appears that the lysozyme activity is directly correlated with the clinical status and severity of the disease. Faecal lysozyme may thus serve as an important tool both in diagnosis and in follow-up in the out-patients clinic for gastroenterology.

Adenocarcinoma↗
PubMed1987

Rapid determination of five coagulation parameters in one sample with a centrifugal analyzer.

Spectrophotometric clotting assays using chromogenic substrates were adapted for use in a routine laboratory batch analyzer. Contrary to another described method the same plasma dilution can be used for each of the different coagulation parameters. Optimal assay conditions were determined. The within- and between-assay variation, the intraindividual variation during 1 day and from month-to-month were determined; the chromogenic assay was compared to a clotting assay. No significant difference (p less than 0.01) between manual and automated methods was found.

Antithrombin III↗
PubMed1985

Short-term and long-term intra-individual variations and critical differences of clinical chemical laboratory parameters.

Twenty eight clinical chemical blood parameters were studied with respect to intra-individual variations. Both short term variations, within one day and during one week, and long term variations, during a six months period, were investigated in groups of 62, 16 and 274 individuals. Moreover, critical differences were calculated from the total variations, i.e. physiological (personal) and analytical variations. The influences of external factors (sex, smoking, and oral contraceptives) were being studied. It was concluded that the use of laboratory data in clinical diagnosis is considerably improved when intra-individual variations and critical differences are used.

Adolescent↗
PubMed1985

Short-term and long-term intra-individual variations and critical differences of haematological laboratory parameters.

The complete blood cell count and the differential leukocyte count have been studied for their variations within-one-day and during one week (short-term variations) and during a period of six months (long-term variations) with an automatic haematology analyser (H-6000, Technicon), in which some new parameters (large unstained cells, high peroxidase cells, red cell distribution width, platelet distribution width, mean platelet volume, plateletcrit) are included. The influence of external factors such as sex-differences, smoking/non-smoking, use of oral contraceptives etc. have also been studied. It appears that the use of critical differences together with haematological data improves the value of these parameters in diagnosis and treatment of patients.

Adolescent↗
PubMed1985

Short-term and long-term intra-individual variations and critical differences of coagulation parameters.

The intra-individual variations of 9 coagulation parameters were studied during short-term (within-one-day) and long-term (six months) periods. Two groups of healthy individuals (viz. 60 and 274 persons) were involved. Moreover, critical differences have been calculated from the total variations, comprising both biological and analytical variations. The influences of external factors such as sex, smoking and the use of oral contraceptives have also been studied. The variability and critical differences found in coagulation parameters in this study appeared to be of the same order as those observed in parameters usually determined in clinical chemistry and haematology. The application of critical differences in the evaluation and use of laboratory data, also in coagulation studies, enhances the objectivity of the judgement. Moreover, in the follow-up of patients the sensitivity of the parameters is increased.

Adolescent↗
PubMed1975

Purification and properties of staphylocoagulase.

Staphylocoagulase, an exoprotein of coagulase-positive Staphylococci, has been purified to a state in which only trace amounts of contaminating proteins are detectable. Aspartic acid was found as a single N-terminal amino acid in this preparation. The molecular weight is 61 000; the isoelectric point lies as pH 4.53. The amino acid composition was determined.

Amino Acids↗
PubMed1975

The influence of cadmium ions on the adsorption of prothrombin onto A1(OH)3 as a means to purify prothrombin.

1. In the presence of CdSO4(1mM),Al(OH)3(1.3% w/v) completely adsorbs the coagulation factors VII, IX, and X from normal plasma, but factor II (prothrombin) is adsorbed for about 50% only.2. A purification procedure for factor II is developed, using Al(OH)3 adsorption in the presence of Cd2+ as a first step and using column chromatography only once. A 750-fold purification is obtained at a 24% yield. 3. Comparison of the prothrombin thus obtained, with prothrombin isolated by the method of Kisiel and Hanahan (Biochim. Biophys. Acta (1973) 304, 103-113) does not show significant differences in amino acid composition, N-terminal amino acid, molecular weight or immunological properties. 4. Comparison of the two prothrombin preparations in a thrombin-generating system shows that although the final yield of thrombin from a given amount of prothrombin in both preparations is the same, the initial velocity of thrombin formation from our preparation is comparable to that of native prothrombin, whereas the other preparation is converted significantly slower.

Adsorption↗
PubMed1975

Activation of a pro-enzyme by a stoichiometric reaction with another protein. The reaction between prothrombin and staphylocoagulase.

The reaction between prothrombin and staphylocoagulase was investigated and the following conclusions were drawn: (a) Optimal amounts of the active reaction product (coagulase-thrombin) are found when equimolar amounts of prothrombin and staphylocoagulase are added together. (b) The molecular weight of coagulase-thrombin equals the sum of the molecular weights of staphylocoagulase and prothrombin when estimated both by gelfiltration and by sodijm dodecylsulphate-polyacrylamide gel electrophoresis. (c) The amino acid composition of coagulase-thrombin cannot be distinguished from the sum of the amino acid compositions of prothrombin and staphylocoagulasd. (d)in a preparation of coagulase-thrombin the N-terminal amino acids are those of prothrombin (alanin) and staphylocoagulase (aspartic acid). (e) An antibody against coagulase-thrombin precipitates prothrombin and staphylocoagulase but not thrombin. (f) We put forward the hypothesis that the thrombin activity in coagulasethrombin is the result of a stoichiometric reaction between one molecule of prothrombin and one molecule of staphylocoagulase, and limited proteolysis does not play a role in this mechanism.

Amino Acids↗