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B M Doronin

Publications and source records attributed to B M Doronin.

10 recordsLinked to original sources
PubMed2005

[A state of immune system in patients with acute stroke].

Over the last years the data on the changes of immunological parameters in traumatic and pathological lesions of brain hemispheres has been obtained by neuroimmunology, a science investigating mechanisms, principles, regularities as well as interaction and integration of immune and nervous systems. However many aspects of the field remain unclear and need further study. In this context parameters of immune system in patients with acute stroke have been examined. These parameters proved to correlate with the disease severity suggesting that the main parameters of cell and humoral patterns of immune system depend on the structural and functional state of brain hemispheres.

Aged↗
PubMed2004

Characteristics of lipids imbalance in patients with tick-borne encephalitis.

Using a novel approach, we have analyzed 30 parameters characterizing detailed spectrum and fractional content of LPs in plasma of patients with tick-borne encephalitis (TBE). The blood plasma of all TBE patients (30 patients), as compared with that of healthy individuals (120 patients), is characterized by decreased concentrations of many LP subfractions and of the total concentration of all plasma LPs (hypolipoproteinemia). The observed difference in some parameters was statistically significant. Using computer-assisted factor analysis, we have shown that according to these 30 parameters TBE patients are similar to patients with multiple sclerosis and systemic lupus erythematosus. The results provide grounds for using data on blood plasma LPs as additional criteria for diagnosis of TBE.

Encephalitis, Tick-Borne↗
PubMed2004

Comparison of enzymatic properties of DNA-abzymes and human DNAses.

DNA-hydrolyzing antibodies (DNA-abzymes, Abz) were shown to be good biochemical markers of some autoimmune diseases such as systemic lupus erythematosus (SLE) and multiple sclerosis (MS). To better understand mechanisms of abzyme generation, one needs to know optimal conditions for DNA hydrolysis by DNA-abzymes, as well as their enzymatic properties in comparison with those of enzymes possessing the same activity. In contrast to human urine deoxyribonucleases, DNA-hydrolyzing antibodies efficiently digested both single- and double-strand DNA. It was shown that polyclonal antibodies (Abs) in MS may contain up to several types of DNase activities, either activated by metal ions or not.

Antibodies, Catalytic↗
PubMed2003

[Association between DNA antibodies levels in the blood of patients with multiple sclerosis and clinical presentation of the disease].

Multiple sclerosis (MS) as an autoimmune disease is characterized by occurrence of high titres of antibodies (AB) to cell structures and autoantigens. Various AB-mediated effector mechanisms can participate directly in the pathogenesis of demyelinisation damaging myelin, oligodendrocytes and nervous fibres. Antinuclear AB, including anti-DNA AB are an example of activation of humoral immunity in MS. Pathogenetic and clinical value of these AB is investigated insufficiently. The aim of this study was estimation of the AB titers in MS patients from two populations of Russia in relation to clinical features of MS. Results of examination of 83 patients with definite MS from Novosibirsk and Moscow (49 and 34 patients) are analyzed. Groups of comparison consisted of healthy donors and patients with SLE of the same age. Use of identical methods in the analysis of the data received in two various populations made the data objective as much as possible and revealed the strongest clinico-biochemical associations. Levels of AB to both native and denaturated DNA were studied. Comparison of several test-systems showed that the system produced by "Specialized scientific Labs" Company has the best sensitivity. In two populations of MS patients the levels of AB in plasma were similar and associated between each other, higher than in donors, but lower than in SLE patients. In both groups MS patients with secondary progressive MS had higher percent of samples of plasma with average and high levels of AB. In the Novosibirsk group associations of levels of AB with parameters of disease severety (EDSS and a number of FS scales) were seen. In the Moscow group levels of AB to DNA were significantly associated with time from the disease onset to certain level of disability (EDSS 3); both the analysis of average values and the correlation analysis showed a weak association of AB to DNA level and MS duration. The data testify that AB to DNA play a more important role in MS pathogenesis than was considered earlier. Catalytic AB as a part of anti-DNA AB may play a special role.

Adult↗
PubMed2001

Catalytic heterogeneity of polyclonal DNA-hydrolyzing antibodies from the sera of patients with multiple sclerosis.

Various catalytic antibodies or abzymes have been detected recently in the sera of patients with several autoimmune pathologies, where their presence is most probably associated with autoimmunization. Recently we have shown that DNase activity is associated with IgGs from the sera of patients with multiple sclerosis (MS) but not with those from the sera of normal humans. Here we present evidence showing that MS IgG, its F(ab) fragments, and separated L-chains catalyze DNA hydrolysis. The properties of the DNase activity of these polyclonal IgGs distinguish them from other known human DNases. In addition, their specific activities with different oligonucleotide substrates and the range of optimal pHs, apparent K(M) values and substrate specificities varied widely for different patients. The findings speak in favor of the generation by the immune systems of individual patients of a variety of polyclonal catalytic IgG pools, from relatively small to extremely large ones.

Antibodies, Catalytic↗
PubMed2001

Vinpocetine treatment in acute ischaemic stroke: a pilot single-blind randomized clinical trial.

The aim of the study was to assess the safety and feasibility of a clinical trial on the effect of vinpocetine, a synthetic ethyl ester of apovincamine, in acute ischaemic stroke. Thirty consecutive patients with computed tomography verified diagnosis of acute ischaemic stroke, who could receive drug treatment within 72 h of stroke onset, were enrolled. The patients were randomly allocated to receive either low-molecular weight dextran alone or in combination with vinpocetine. Poor outcome was defined as being dead or having a Barthel index of < 70 or a Rankin score of 3--5. Intention-to-treat analysis was applied. One-tenth of all hospitalized patients with acute ischaemic stroke were eligible for the trial. Thirty eligible patients were treated with either low-molecular weight dextran alone (mean age 57.9 +/- 11.6 years, n = 15) or in combination with vinpocetine (mean age 60.8 +/- 6.6 years, n = 15). The two treatment groups were comparable with respect to major prognostic variables. A relative risk (RR) reduction of poor outcome at 3 months follow-up was 30% (RR = 0.7; 95% confidence interval [CI] 0.1--3.4), as defined by the modified Barthel Index, and 60% as defined by the modified Ranking score (RR = 0.4, 95% CI: 0.1--1.7). The National Institute of Health (NIH--NINDS) Stroke Scale score was marginally significantly better in the vinpocetine treated group at 3 months of follow-up (P = 0.05, ANOVA). No significant adverse effects were seen. This pilot study shows that a full-scale randomized double-blind, placebo-controlled trial of vinpocetine treatment in acute ischaemic stroke is feasible and warranted.

Acute Disease↗
PubMed1989

[Clinico-immunologic parallels in multiple sclerosis].

HLA antigens were compared in groups of patients with disseminated sclerosis and healthy donors. In remission group as compared with controls, increased were the rates of A9, B7, B13, B35, A0/B17, A1/B7, A9/B7, A3/B35, A11/B7, A11/B8, B5, B7, 15, and in the progredient group--B7, Bw22, B35, A1/B7, A2/B7, A2/B35, A9/B7, B7, 12. These groups were different only in A11/B7 rates. Remitting and progredient course of the disease in women differed in A3 and A10 rates, in men in A2 and A11. In men with remitting course the A2 antigen rate was substantially lower than in any other group. With respect to the disease duration and the degree of patients's invalidization, the groups of benign, malignant, and intermediate course were singled out differing from each other and controls also in their HLA composition.

Adolescent↗
PubMed1988

[Immunogenetic markers of disseminated sclerosis in western Siberia].

The contents of class 1 HLA antigens was compared in patients with disseminated sclerosis, their first-grade relatives and healthy donors. Patients, as compared to healthy persons, had higher occurrence rate of A1, A9, B7, A0/B0, A9/B7, A1/B7, A1/B12, A2/B7, B7, 27, B5, 0, B7, 12, B7, 15, B5, 7, and lower A28, B13, Bw22, B35, 40, B7, 35, A2, w19, A0/B35, A0/B13, A2/B15, A3/B35. In male patients, as compared to female patients, B40 and A1/B15 were more frequent, and B12 less frequent. The subgroup of patients with the diseases onset before 20 years considerably differed from other patients in their rates of A1, A1, 2, A1w19, A1/B0 increased and A3 and A9/B17 decreased. Compared to donor group, the patients' relatives had differences similar to those between patients and relatives. High relative risk values were characteristic of some of the above associations.

Adult↗
PubMedDate not supplied

[Antibodies to DNA in the blood of patients with tick-borne encephalitis].

A comparison of relative levels of autoantibodies (Abs) to both native (n) and denatured (d) DNA in the blood of 55 patients with tick-borne encephalitis (TBE) was carried out. 31% of patients with TBE was shown to have an increased level Abs to nDNA and 40% of patients demonstrate increased level of Abs to dDNA. The percent of TBE patients with increased concentration of anti-nDNA Abs higher then that in patients with multiple sclerosis (18%) and some other autoimmune diseases (6-18%), but comparable with that for patients with systemic lupus erythematosus (38%) and polymyositis (42%). In contrast to patients with systemic lupus erythematosus and multiple sclerosis, the level of Abs to nDNA in patients with TBE is higher than the level of Abs to dDNA. Correlation coefficients of Ab levels to both n- and dDNA were estimated for group of patients in whole and for separate subgroups with different type of disease (temperature reaction, feverishness and meningitis). Analysis of correlation between titres of anti-DNA Abs and three standard biochemical markers of TBE (activity of aspartate- and alanine-aminotransferases, and concentration of whole bilirubin) for 22 patients with TBE was carried out. Statistically significant correlation was revealed only between the level of Abs to nDNA and activities of aspartate- and alanine-aminotransferases, correlation coefficients are equal to +0.44 and +0.48, respectively.

Antibodies, Viral↗