Biomedical subjects
Publications and source records attributed to B M Michov.
A method of electrophoresis in an expanded stationary boundary is described, in which a pH gradient formed in a single buffer is used. The degrees of ionization and the concentrations of the electrolytes have values at which the polyions stack and resolve simultaneously in a polyacrylamide gel. To illustrate the method, the resolving of serum proteins in a pH gradient formed by Tris-glycinate buffer is considered.
The existence of two radii of each charged particle-a geometric and electrokinetic radii, is supposed. The mathematical relationship between them in the four possible combinations of an ion and its counterion is analyzed: (i) at equal geometric radii and, in absolute values, equal valencies; (ii) at equal geometric radii and, in absolute values, different valencies; (iii) at different geometric radii and, in absolute values, equal valencies; (iv) at different geometric radii and, in absolute values, different valencies. One of the equations worked out can be used to define the relationship between the geometric and electrokinetic radii of a polyion. All the equations are used in working out precise calculations.
A theory for discontinuous electrophoresis in a polyacrylamide gel is presented for one buffer at two pH values. It is shown that polyions stack between identical leading and trailing ions, and resolve in a gel of constant polyacrylamide concentration. The theory is illustrated by the separation of serum protein polyions in a Tris-glycinate buffer of pH 8.19 in the well-forming gel, and pH 9.16 in the resolving gel. The selected concentrations and electrolyte ionization degrees of Tris and glycine have values at which the serum protein polyions stack between the resolving and electrode buffers, followed by separation in the resolving gel.
The mobilities of the "threeborate" ion at different temperatures and ionic strengths are calculated using previously published equations for calculating the mobilities of composed ions.
We recently established that every ion has two radii - a geometrical one and an electrokinetic one. Using this principle, and in accordance with Henry's method of determining his function, f(ka), we have discovered that the Henry function can be radically simplified. The graphs of the proposed equation are plotted at different argument values. They are identical with the graphs plotted according to the Henry function.
A quantitative theory of the Stern electric double layer is suggested. It is based on the view that every ion possesses a geometrical and an electrokinetic radius, that the ionic atmosphere begins from the geometrical one, and that the difference between these radii is the Stern quantity delta. The equations of the mentioned radii and the quantity delta are established and the values of the different potentials characterizing an ion and its ionic atmosphere are determined.
Explore the source record for details and available documents.