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Biomedical subjects

B M Vose

Publications and source records attributed to B M Vose.

At least 55 records · Page 3Linked to original sources
PubMed1977

Leukocyte migration inhibition in human pulmonary neoplasia.

Peripheral blood leukocytes from 65 lung cancer patients, 69 healthy donors, 33 patients with malignant disease outside the lung, and 24 patients with nonmalignant pulmonary conditions were examined for immune reactivity, as measured by the leukocyte migration inhibition assay, to antigens in homogenates of lung tumor tissue and material derived from tumor-free lung areas and a nonmalignant lung lesion. A low level of reactivity with apparent specificity was detected in the lung cancer patient group, with 17/52 showing migration inhibition with extracts of lung tumor tissue. Reactivity was also detectable in this group against homogenates of tumor-free lung and nonmalignant lung lesion. The data supported the conclusion that sensitization at least in some patients with lung cancer might be directed not only against tumor-specific antigens but also against antigens of less confined disease association.

Adult↗
PubMed1977

The effects of halothane anaesthesia on antibody-dependent cellular cytotoxicity in rats.

Lymphocytes from rats killed while anaesthetized with 1-5 per cent halothane showed a significantly reduced capacity to induce lysis of antibody-coated target cells compared with those from untreated rats. This effect was short-lived however, being no longer apparent in lymphoid cells taken from rats 2 h after their recovery from such anaesthesia. Surgical procedures were not effective in extending the duration of reduced ADCC activity. The implication of these findings is that the post-operative depression of this in vitro assay of immunocompetence results from the influence of the anaesthetic agent per se or its metabolities with surgical trauma having little role.

Anesthesia, General↗
PubMed1976

Post-operative depression of antibody-dependent lymphocyte cytotoxicity following minor surgery and anaesthesia.

Leucocytes taken 1 day post-operatively from patients who had undergone surgery under general anaesthesia for benign breast disease showed a significantly diminished capacity to induce lysis of antibody-coated target cells compared with those taken pre-operatively from the same patients. No significant fall in PHA responsiveness was observed in these leucocytes in the post-operative period. This indicates a high sensitivity of the cell types involved in the antibody-dependent cell-mediated cytotoxicity reaction to the suppressive effect of surgery and anaesthesia. Plasmas taken post-operatively from these patients were effective in diminishing the capacity of leucocytes from healthy untreated donors to initiate antibody-coated target cell lysis compared with pre-operative plasmas although the plasma cortisol levels in these samples did not differ significantly. Possible mediators of this suppressive effect and its significance are discussed.

Anesthesia, General↗
PubMed1975

Cell-mediated cytotoxicity to human pulmonary neoplasms.

Peripheral blood leukocytes from patients with confirmed pulmonary neoplasia were tested for cytotoxicity against cultured cells derived from lung tumours of various histological types, foetal and normal adult lung tissue and tumours arising in organs other than the lung. Leukocytes from 73 percent of patients were cytotoxic for lung-tumour derived cells compared with age- and sex-matched normal donors, while the frequencies of reactivity against normal adult lung-derived cells and cells from unrelated tumours (e.g. bladder, colon, breast) were 42 percent and 18 percent respectively. Leukocytes from lung cancer patients were also cytotoxic for cells derived from foetal lung but susceptibility to cytolysis was variable, cells from 13- and 14-week embryos revealing greatest reactivity (88 percent). Leukocytes from patients with a variety of tumours of non-pulmonary origin or with non-malignant conditions (including respiratory disorders) were also reactive with lung-tumour-derived target cells but with a lower overall frequency (35 percent) than those from lung-cancer patients. The significance of these cytotoxicity data for the existence of tumour-specific host immunoreactivity in lung neoplasia is discussed.

Adenoma↗
PubMed1975

Effect of surgery on tumour-directed leucocyte responses.

Leucocytes from 22 out of 26 patients with mammary carcinoma were significantly cytotoxic in vitro for cells cultured from mammary tumours though only two out of 17 of these preparations were cytotoxic for cells cultured from tumours arising at other sites. In the immediate postoperative period reactivity of patients' leucocytes with mammary tumour cells was undetectable but returned within one week of surgery. Leucocyte cytotoxicity may therefore offer a model in which the mechanism of postoperative depression of immunological competence may be investigated.

Antibodies, Neoplasm↗
PubMed1975

Leucocytotoxicity in malignant and non-malignant colonic diseases.

The survival of cells from five different cultures of allogeneic malignant colonic carcinoma, two from normal adult colonic epithelium and eight from foetal colonic epithelium in the presence of leucocytes from patients with neoplastic and inflammatory disorders of the colon has been compared. Cytotoxicity assessed by the reduction of the number of adherent target cells in microplate wells compared with those surviving in wells treated with tissue culture medium alone was observed with leucocytes from donors in all categories examined including those from individuals without any known abnormality. Patients with ulcerative colitis were the only group to reveal consistent reactivity against cultures derived from all three sources, an observation which may reflect sensitization to organ-related antigens in this disease. In contrast, leucocytes from patients with bowel neoplasia showed reactivity for cells derived from colon carcinoma tissue, which was comparable to that of healthy donors. Evidence for tumour-specific cytotoxicity was therefore lacking in this study. It is suggested that the detection of tumour-associated antigens on cultured cells may be limited by a number of factors of which the wide variation in reactivity among controls and unspecified nature of the target cells are likely to be of greatest importance.

Antigens, Neoplasm↗
PubMed1974

Differentiation between the embryonic and tumour specific antigens on chemically induced rat tumours.

The tumour specific antigens and tumour associated embryonic antigens expressed on chemically induced rat hepatomata and sarcomata have been shown to be different by means of blocking antibody studies. Serum from multiparous female rats contained blocking antibody which protected both tumour and embryo cells from the in vitro cytotoxic effect of lymph node cells from multiparous donors. These sera did not, however, block the cytotoxicity of lymph node cells from tumour immune rats for cells of the immunizing tumour. In addition, the tumour specific rejection antigens and embryonic antigens have been shown to have dissimilar locations in tumour cells.

Animals↗
PubMed1974

Embryonic antigen expression on 2-acetylaminofluorene induced and spontaneously arising rat tumours.

2-Acetylaminofluorene induced mammary and ear duct carcinomata and spontaneously arising mammary carcinomata and sarcomata were shown to express embryonic antigens at the cell surface by their reaction with serum from multiparous female rats. These observations with essentially non-immunogenic tumours are comparable with early findings showing that embryonic antigens are also expressed on aminoazo dye induced rat hepatomata, and sarcomata induced with 3-methylcholanthrene. Re-expression of embryonal components, therefore, may be a concomitant of neoplastic transformation.

Acetamides↗