PubMed Health⌕ Search

Biomedical subjects

B M Wu

Publications and source records attributed to B M Wu.

25 records · Page 2Linked to original sources
PubMed1991

Analysis of the 5' flanking region of the human beta-glucuronidase gene.

We have sequenced 4.2 kb of the 5' flanking region of the human beta-glucuronidase gene, compared this sequence to the 5' upstream sequence reported for the murine gene, determined the transcription start sites of the human gene, and studied expression of human minigene deletion constructs in COS cells. The 200 bp immediately 5' to the translation initiation codon have a high G + C content (72%) and contain no TATA box, two properties commonly associated with "housekeeping genes." The sequence 5' to -200 bp contains seven Alu repetitive elements which account for more than 50% of this flanking sequence. From deletion analysis of minigene constructs, 200 bp of 5' sequence appeared sufficient for maximal expression in transfected COS cells. S1 nuclease protection analysis showed that transcription initiates from a cluster of sites around -30 bp in all tissues examined. In some cases, a low but detectable level of transcription also initiates 126 bp upstream of the ATG. Inspection of the sequence surrounding both start sites revealed some similarity to the recently described "initiator" transcriptional control element (S.T. Smale and D. Baltimore (1989), Cell 57: 103-113). Comparison of the 5'flanking sequence with that available from the murine beta-glucuronidase gene reveals only one 28-bp highly conserved region, which surrounds the -126 start site.

Amino Acid Sequence↗
PubMed1989

[A comparative study of EEG in patients with neurological and myxedematous cretinism].

A comparative study of EEG between neurological and myxedematous cretins was carried out in a total of 61 cases. The results were as follows: (1) The changes of EEG in myxedematous cretins appeared to be more obvious than those in neurological cretins; (2) The degree of mental retardation in cretins was not parallel to the degree of the abnormality of EEG; (3) with thyroid therapy, EEG in cretins was improved. The improvement rate in myxedematous cretins was higher than that in neurological cretins; (4) Brain CT scan was carried out in 50 cases of those cretins. It was found that the abnormality of certain intracerebral structure in myxedematous cretins appeared to be more obvious than that in neurological cretins. The change of EEG appeared in the same way as well. This is in contradiction to the traditional knowledge that the defect of neurological system in neurological cretinism was more severe than that in myxedematous cretinism. The current classification of cretins into neurological and myxedematous cretins does not reflect the changes of pathophysiology properly.

Adolescent↗
PubMed1980

Female cadaver in the Han Tomb No. 1. X-ray diffraction studies on two kinds of fibrous protein.

The molecular structures of both native and false hairs as well as the tendon of the female cadaver buried about 2,100 years ago, which was excavated from the Han Tomb, No. 1 at Mawangdui near Changsha, Human Province, China, have been studied with X-ray fiber diffraction. It has been shown that the conformations of alpha-keratin and collagen and the basic longitudinal period of these fibrous protein molecular chains have been preserved in those specimens respectively, but the aggregations of fibrous molecules have somewhat changed. Some exploration was made on the origin where came the cubic mercury sulphide inside the cadaver's hair.

Cadaver↗