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Biomedical subjects

B Machnicka

Publications and source records attributed to B Machnicka.

At least 37 records · Page 2Linked to original sources
PubMed1985

Treatment with praziquantel the Taenia saginata infection in men.

Praziquantel given in single doses of 12, 15 and 20 mg/kg body weight was applied in treatment of Taenia saginata infection (61 persons) and in four doses against Hymenolepis nana infection (1 person). Only one person not being cured shows that the compound has given a very high cure rate in clinical trial. 10% of patients treated complained of subjective mild side effects, which disappeared during 24 h after drug administration.

Humans↗
PubMed1983

Immune response and immunodepression in Hymenolepis diminuta infection in rats.

Maximum size range of Hymenolepis diminuta was found in rats 22 days postinfection (p.i.) with single or several (3-6) tapeworms. Immunologic reactions of the host were connected with the growth and development of parasites. Positive results of macrophage migration inhibition test (MMI) were recorded from 7 until 22 days p.i. in infection with a single and to 16 days p.i. in those with 3-6 H. diminuta. The negative results of the MMI test observed at later postinfection times are considered as a manifestatioin of immunosuppression caused by the presence of H. diminuta. The expulsion of tapeworm by anthelmintic treatment with Yomesan restored for a short time cell-mediated immunity, as demonstrated by MMI test. The level of antibodies, as determined by passive hemagglutination test (HA), varied during the infection; it was highest at 22 days p.i. The removal of tapeworms as a result of drug treatment caused a rise in titers.

Animals↗
PubMed1981

Intradermal test in the diagnosis of bovine cysticercosis (Cysticercus bovis).

The skin test was performed in cattle infected with C. bovis. The examination were carried out on naturally (23 head) and artificially infected animals (20 head) at different times after infection. Antigens prepared from T. saginata (B1, N0.1, C, Calk) and antigens extracted from activated oncospheres of T. saginata (OI and OII) were inoculated intradermally. The most active were the antigens B1, N0.1, and C prepared from T. saginata. In the majority of infected animals the antigens produced the immediate type of reaction developing the maximum intensity during 2 h post injection. It is worth mentioning that Arthus-type responses have often been observed. Uninfected animals when tested with the same antigens developed mild reaction, not exceeding 4 mm skin thickness. The second testing of the same animals after one or five months resulted in a more intensive reaction slightly resembling that one observed in infected animals. There is no correlation between titre of passive haemagglutination test and the skin test reaction in naturally infected animals. Neither the number of cysticerci with which the animal is infected has an influence on the degree of the skin edema.

Animals↗
PubMed1981

Development of oncospheres of Taenia saginata after a concomitant infection by oral and subcutaneous routes.

Two experimental bulls (weight 200 kg each) were infected with activated oncospheres of Taenia saginata. The infective dose (160,000 oncospheres) was administered twice to each animal, once by oral, once by subcutaneous routes. At 6 hr p.i., a boil appeared at the site of puncture which attained a diameter of 5.5 cm and 6.2 cm respectively within 3 days, and a thickness of 1.8 cm and 2.2 cm. The postmortem disclosed an extremely weak total infestation of the muscles (approximately 30 cysticeri). The inflammatory tissue of subcutaneous foci contained eggs with an embryophore. Part of the eggs were enclosed in multinucleate giant cells. The tissue reaction differed in dependence on the viability of the oncosphere in the egg. Either giant cells were produced as in the presence of a foreign body, or there was a local accumulation of eosinophiles which after their destruction, were replaced by a crystalline conglomerate containing an egg in its centre. In addition, we found young stages of cysticerci affected by dystrophy. Remarkable eosinophile substances observed on the surface both of some eggs and of developing cysticerci (an analogy of the Splendone-Hoepli phenomenon) apparently indicated a binding of antibodies to the antigenic structure.

Animals↗
PubMed1979

Tissue reaction in the heart of cattle with a spontaneous and artificial Cysticercus bovis infection.

Results of detailed studies on tissue reactions to Cysticercus bovis in the heart of cattle, together with a comparison of findings in animals with spontaneous and experimental infection, and an evaluation of tissue reactions in relation to the location, morphology and morphogenesis of C. bovis provided evidence for the fact that in general, the response of the heart to the presence of C. bovis was an inflammatory reaction characterized by the origin of a pseudoepithelial border and a zone of granulation tissue. Later, when necrotic changes started to affect the cysticercus, the inflammatory reaction started to develop anew. It was accompanied by an exudation and a subsequent resorption. Characteristic features were focal necroses both of the exudate and the inflammatory border followed by a dystrophic calcification, focal necroses and a dystrophic calcification of individual collagenous fibres and groups of these fibres in addition to a regular appearance of necrotic-like foci typical of a reaction to C. bovis. The location of the cysticercus in a certain part of the lymphatic system of the heart and in skeletal muscles was shown to have a considerable effect on the course of the inflammatory reaction. Differences in the development of the inflammatory reaction explained concomitant findings of dead and live cysticerci at the same time after an experimental infection.

Animals↗
PubMed1979

Tissue reaction to Cysticercus bovis in the lung of artificially infected cattle.

The tissue reaction to Cysticercus bovis in the lung of cattle with an experimental infection was an inflammatory rim originating in the immediate vicinity of the cysts. The cysts recovered at days 83 and 102 p.i. contained living cysticerci. The rim was composed either of a layer of high histiocytes organized in palisades (at day 83 p.i.), or a lyer of flat histiocytes (at day 102 p.i.). The outer layer of the rim consisted of fibroblasts, reticular cells and a different number of eosinophil- and neutrophil luekocytes. On the periphery, the rim was formed by granulation tissue infiltrated with lymphoplasmocytes. At the border between the layers of the inflammatory rim there were conspicuous foci of a necrotic appearance typical of a tissue reaction to C. bovis.

Animals↗
PubMed1978

Histochemistry of tissue reaction in skeletal muscles of cattle experimentally infected with Cysticercus bovis.

A close correlation between the intensity of tissue reaction in skeletal muscles and the localization of some enzymes in the bladder of C. bovis was demonstrated by histochemical methods. The most intensive tissue reaction was observed around the portion of bladder surrounding the opening of spiral canal, the tegument and subtegumental cells of which exhibit a high activity of alkaline phosphatase and acid phosphatase. Around this portion of bladder the tissue reaction is very strong, whereas around the remaining portion of the bladder, without any activity of these enzymes, the reaction is weak. The basic type of the reaction around the portion with alkaline and acid phosphatase activity is the formation of a pseudoepithelial rim, in which occur secondary changes leading to histochemical changes inside and around this rim. The cells of the unchanged pseudoepithelial rim contain proteins with tyrosine, tryptophan and cysteine. Among the cells is a large number of reticular fibres. Flat foci localized directly in this rim contain mostly fibrilar structures rich in acid mucosubstances with carboxyl and sulphate groups which are labile to testicular hyaluronidase and neuraminidase. They contain also a small amount of neutral mucosubstances and give negative reactions for tyrosine, tryptophan and cysteine. Fibrilar structure in these foci undergo dystrophic calcification. A conspicuous accumulation of mast cells is visible in the layers under the pseudoepithelial rim and clusters of cells containing lipopigment are present at the periphery of the connective tissue layer.

Animals↗