Improvement of routine estrogen and progesterone assay: double labeling with 125-I-labeled estradiol and [3H]R5020.
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Breast Neoplasms↗
Biomedical subjects
B Manz
Publications and source records attributed to B Manz.
A comparison of cytoplasmic and nuclear estradiol and progesterone receptors in human fallopian tube and endometrial tissue.
Quantitative and qualitative aspects of the in vitro binding of 3H-estradiol and 3H-progesterone to receptor components from human endometrium and fallopian tube cytoplasmic and nuclear fractions were studied. The steroid binding macromolecules formed in vitro could be extracted from nuclei by 0.4 M KCl and detected by glycerol gradient centrifugation. Both estradiol- and progesterone-binding compounds formed only one peak (under high ionic strength conditions) with a sedimentation coefficient of about 4-5 S. The number of cytoplasmic and nuclear binding sites for both estradiol and R5020 varied dramatically throughout the menstrual cycle: the estradiol and progesterone receptor concentrations were highest during the proliferative phase and were very significantly lower in the second half of the menstrual cycle. Furthermore, measurement of both receptors in the cytosol revealed differences among the anatomic segments of the fallopian tube. The highest estradiol and progesterone binding could be detected in the ampullary region; significantly lower levels of estradiol and progesterone receptors were seen in the infundibulum and the isthmus.
Cell Nucleus↗
Three-step purification of glucocorticoid receptors from rat liver.
The 90 000-Mr glucocorticoid receptor was purified to homogeneity according to sodium dodecylsulfate/polyacrylamide gel electrophoresis. An affinity column containing either dexamethasone-17 beta-carboxylic acid or dexamethasone-21-methanesulfonate bound to the matrix with the help of a disulfide bond is used in this study. Using this affinity matrix, in a single step, 8700-fold purification of glucocorticoid receptor from rat liver cytosol could be achieved. Following the method of activation and DNA-cellulose chromatography the 90 000-Mr receptor subunit was purified to homogeneity.
Animals↗
Synthesis of 21-diazo-9 alpha-fluoro-16 alpha-methyl-21-deoxy-[1,2(n)-3H]prednisolone.
The preparation of 21-diazo-9 alpha-fluoro-16 alpha-methyl-21-deoxy-[1,2(n)-3H]prednisolone is described. [1,2(n)-3H]Dexamethasone 26 Ci/mmol, was oxidized with cupric acetate/air to give the 21-aldehyde. Treatment of the aldehyde with hydroxylamine gave the corresponding 21-oxime which was reacted with chloroamine to give a 25--35% yield of 21-diazo-9 alpha-fluoro-16 alpha-methyl-21-[1,2(n)-3H]deoxyprednisolone with a specific activity of 25--26 Ci/mmol.
Animals↗
[Clinical picture of acute intermittent porphyria with reference to morphological findings].
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Adult↗
Faculty perceptions of teaching on television: one school's experience.
Videoteleconferencing (or teaching on television) represents a means of growing importance for distant learners to gain greater access to nursing education. Research has identified a number of key issues for nurse educators to consider related to videoteleconferencing: teaching/evaluation strategies, faculty workload, rewards and incentives, and support structures. The authors conducted an evaluation survey using a case study approach aimed at describing the perceptions of a convenience sample of faculty members (n = 17) regarding their recent television experiences. The survey, conducted by means of a mailed questionnaire, examined perceptions centering on sources of support, reception site logistics, rewards teaching strategies, and future directions. Key findings included: overall, respondents perceived television teaching positively; reception site, administrative support, and peer support were important to faculty; additional preparation time was required for television teaching; teaching on television required changes in style and pedagogical strategies; and student-teacher interactions were affected by the medium. Findings have implications for the implementation of videoteleconferencing operations, recruitment of faculty for television teaching, and evaluation of videoteleconferencing operations.
Attitude of Health Personnel↗
Characterisation of fluid flow through porous media using three-dimensional microimaging and pulsed gradient stimulated echo NMR.
Pulsed gradient stimulated echo (PGSTE) and microimaging nuclear magnetic resonance (NMR) are used to probe correlations between structure and flow in the void space of a model porous system formed from a packing of 1-mm diameter glass spheres. The pulsed gradient stimulated echo data determine the average propagator and permit the dispersion of the flow to be studied as a function of delay time. Microimaging yields structural information and, specifically, a reduced radial distribution function (rdf) for the structure of the void space. Transition to fully developed dispersive flow is shown to occur on a scale size for which no further correlations in the structure of the void space are observed.
Glass↗