Biomedical subjects
Publications and source records attributed to B Meignier.
The industrial usefulness of the production of foot and mouth disease virus with microcarrier grown cells has been tested at a large scale. The vaccines, intended for pigs, prepared with such virus give a good level of immunity against virulent challenges. They are stable and free from side effects.
The microcarrier culture technique has been applied to a pig kidney cell line. Microcarriers consisted of DEAE Sephadex A 50 beads washed and containing carboxymethyl cellulose. Sifted beads gave better results than unclassified material. Cells for inoculum were prepared in Roux flasks. The two types of fermentors which were used (operating capacity 100 l and 150 l) gave similar results. The growth of the cells can easily be followed by microscopic observation and cell count. The yields of cells per volume of spent medium were three times higher on microcarrier than in Roux bottles; the average doubling time was not modified. Foot-and-mouth disease virus can be produced with cells grown on beads. Vaccines prepared with these viruses induced good protection for pigs.
Two recombinant herpes simplex viruses, of type 1 background, were constructed with two large deletions and duplicate sets (type 1 and type 2) of the genes coding for glycoproteins D, G, and E. One recombinant (R7020) is thymidine kinase-positive, and the other (R7017) is thymidine kinase-negative. Evaluation in rodents indicated that these viruses are genetically stable, capable of establishing latency, protective against severe herpetic diseases, and protective against the establishment of latency. In Aotus monkeys, R7020 replicates at the site of inoculation but does not disseminate in the body. It can reactivate from the latent state but without causing recurrent lesions, even in immunosuppressed monkeys.
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