The sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. All strains of Shigella sonnei (17), S. flexneri (17), S. boydii (16), Escherichia coli enteropathogen (40), E. coli enteroinvasive (20), Campylobacter jejuni (50), Vibrio cholerae 01 (5), Vibrio cholerae non 01 (5), V. parahaemolyticus (5), V. alginolyticus (2), Aeromonas hydrophila (5), A. caviae (5), A. sobria (5), and Plesiomonas shigelloides (12) were sensitive (MIC < or = 8 mg/l), except a strains of E. coli enteropathogen (MIC 16 mg/l). The 15.5% of the 51 strains of Salmonella enterica belonging to the type I isolated between 1987 and 1989 were resistant (MIC > or = 16 mg/l). A similar degree of resistance was observed in 20 strains of this agent isolated between 1978 and 1980. The 30 strains of Yersinia (including 15 strains of Y. enterocolitica 03) presented extreme values of MICs of 8 and 16 mg/l.

Dose-Response Relationship, Drug↗

PubMedDate not supplied

[Etiology of enteritis in a university general hospital in Barcelona (1992-1995)].

BACKGROUND: The aim of the study was to describe the etiology of enteropathogenic agents over a four-year period (1992-1995) in a University Hospital in Barcelona. METHODS: We studied 12,793 stool samples, 4519 were obtained from patients under 15 years and 8274 were obtained from patients over 14 years. The specimens were examined for bacteriological, parasitological and virological enteropathogens. RESULTS: In 3380 specimens of 12,793 stool samples studied were identified an enteropathogen (26.4%). Polymicrobial associations were observed in the 6.8% of the cases. Pathogens were identified in 45% of children samples and 16.3% of adults samples. The etiological enteritis agents more frequently detected in the paediatric patients were Campylobacter (13.5%), rotavirus (11.3%) and Salmonella (10.2%); and Salmonella (4.9%), Campylobacter (3.1%) and Giardia intestinalis (2.1%) in adults. Cryptosporidium (13.5%) was the most frequent cause of gastrointestinal tract infections in HIV-infected subjects. In the children with stools positives, the presence of red and white blood cells were more frequent than the adults with stools positives (73% versus 26.6%). CONCLUSIONS: The enteropathogenic agents such as Campylobacter, Salmonella, and Giardia were the most frequent cause of gastroenteritis in our environment. In the children, rotavirus infections predominated during the cold months. The most frequent cause of gastroenteritis in HIV-infected patients was Cryptosporidium followed by Campylobacter.

Adolescent↗

PubMedDate not supplied

[Detection of pathogenicity factors in strains of classical enteropathogenic Escherichia coli].

OBJECTIVE: To determine the number of strains of classic enteropathogenic E. coli (EPEC) that have the eae gene, that is considered a pathogenicity factor. MATERIAL AND METHODS: The presence of the eae gene has been evaluated on 62 EPEC strains of ten different serogroups, isolated from children with gastroenteritis. RESULTS: Amplification of the eae gene was positive in 10 out of 62 EPEC strains analyzed (16%) corresponding to seven different serogroups. DISCUSSION: The low frequency of the detection of the eae gene on EPEC strains shows the limited correlation between the pathogenicity and the serogroup of the strains and would corroborate the need to reexamine this subject prospectively in our country.

Adhesins, Bacterial↗