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Biomedical subjects

B Musiatowicz

Publications and source records attributed to B Musiatowicz.

At least 19 recordsLinked to original sources
PubMed1999

Study of borderline and invasive mucinous ovarian tumors using Ki-67 (MIB 1) antibodies and nucleolar organizer region (NOR) staining.

Extensive application of new methods in histopathology has resulted in large quantities of data on molecular markers in different types of human tumors. The main purpose has been to obtain additional parameters for the characterization of various types of malignancy to give more precise information on their biological behavior. In this study we tested AgNOR and Ki-67 (MIB 1) staining in 39 ovarian mucinous tumors to evaluate its diagnostic potential in distinguishing between borderline tumors and well-differentiated carcinomas at stage I of FIGO classification. In general, there was an increase in AgNOR median values from benign (1.83 +/- 0.8) to well-differentiated carcinomas (2.91 +/- 1.3) with an intermediate value in borderline (2.3 +/- 1.6). The lowest Ki-67 labeling index (LI) was found in benign cystadenomas (5.7% +/- 3.4%) and papillary mucinous cystadenomas (7.9 +/- 5.8%). In borderline tumors mean LI was 12.2 +/- 10.9% whereas well-differentiated carcinomas had higher LI (16.8 +/- 10.2%). There were no statistically significant differences between the evaluated tumors and techniques applied. In our opinion the AgNOR count and Ki-67 LI do not appear useful for assessing differences between borderline and well-differentiated mucinous ovarian tumors at stage I of clinical advancement.

Adenocarcinoma, Mucinous↗
PubMed1999

[Tumor DNA content as a prognostic indicator in squamous cell carcinoma of the larynx].

Celluar DNA content has been found to be an important prognostic factor in many head and neck tumours, yet within the larynx few studies have examined its effect on survival. 31 patients with squamous carcinoma of the larynx treated in years 1983-85 by total laryngectomy were studied. The tumours were classified according to the TNM classification 1987 and graded histopathologically according to Jaccobson's classification. Quantitative DNA content was determined with the use of image cytometry performed by an image analyzer (CAS-200). Quantitative DNA analysis correlated with tumor grade and patient outcome within 6 years of follow-up.

Carcinoma, Squamous Cell↗
PubMed1999

[Second primary neoplasms in laryngeal cancer patients].

In the period of 1990-1992, 238 patients underwent surgical treatment for laryngeal carcinoma in ORL Department of Medical Academy in Białystok. Until now 27 cases of second primary malignances (SPM) have been found in this group (11.3% of the screened population). Upper aerodigestive tract was the most frequent location, i.e. it was observed in 18 cases (66.6%). There was 100% mortality among patients with SPM of stomach, oesophagus and lung estabishing 13% deaths in the whole group. There was no correlation between the stage and the location of primary laryngeal cancer and SPM. Carcinogenic influence of smoking and alcohol on primary malignancies and SPM within upper aerodigestive tract development has been confirmed.

Adult↗
PubMed1999

[The use of exfoliative cytology in the detection of dysplastic changes in the laryngeal mucosa of postop cancer patients].

Second primary neoplasms are the cause of failure in treatment of head and neck carcinoma in 1-34% of cases. The majority of SPM are located within mouth, pharynx, lung or oesophagus. This study was designed to evaluate to effectiveness of exfoliative cytology in postoperative follow-up of patients with carcinoma of the larynx treated with surgery. The group consisted of 81 patients who had cytological evaluation between 1994 and 1996. Cytological smears were taken from six different places in mouth, pharynx and tracheostoma. If cytomorphotic changes, parakeratosis or inflammation was found, the examination was repeated every 6 months. In the first study among 548 smears cytomorphotic changes were found in 16 (2.91%) taken from 8 patients (9.87%). 9 smears presented small or medium degree of dysplasia, 4-severe degree of dysplasia, and 3 smears demonstrated neoplastic cells. Upon completing second and third cytologic examination full remission was found in cases of small and medium dysplasia as in parakeratosis and inflammation. Stagnation was detected in cases of severe dysplasia. In the patient whose smear revealed neoplastic cells SPN of the oesophagus was diagnosed. It seems that exfoliative cytology might be a useful method for follow-up and early diagnosis of SPN in patient after laryngectomy.

Cell Transformation, Neoplastic↗
PubMed1999

[Surgical management in the non-neoplastic and neoplastic thyroid goiter with Hürthla cells].

The routine preoperative cytological evaluation of pathological changes of the thyroid more and more frequently reveals single or multiple Hürthl cells in the material obtained by the method of fine-needle aspiration biopsy (FNA). Hürthl cells are a degenerative form of epithelial follicular cells of the thyroid and cytological examination is insufficient for unequivocal differentiation between adenoma and carcinoma. Management of patients with FNA-diagnosed Hürthl cells arouses a number of controversies. Therefore, the authors of the present study subjected the thyroids of patients with cytologically diagnosed Hürthl cells to postoperative histopathological analysis. Appropriate surgical management was indicated.

Adenoma, Oxyphilic↗
PubMed1999

[Analysis of the frequency of prevalence of endometrial hyperplasia and endometrial adenocarcinoma based on own material in the year 1975, 1985 and 1995].

We analysed the frequency of prevalence of endometrial hyperplasia and endometrial adenocarcinoma in the years 1975, 1985 and 1995. Significant differences between endometrial simple hyperplasia and complex hyperplasia with atypia was found. There were no statistic differences in the frequency of the prevalence of endometrial adenocarcinoma between analysed years.

Adenocarcinoma↗
PubMed1999

[Immunohistochemically expression of the protein p53 evaluated in invasive squamous cell carcinoma of the cervix].

The p53 protein expression in the invasive squamous cell carcinoma of the cervix was evaluated immunohistochemically. Its predictive role in the correlation with histological grading and staging was analyzed. 13.7% of examined cases (10/73) presented positive protein expression. There was no correlation between the intensified protein p53 expression and particular prognostic histological factors.

Adult↗
PubMed1998

The effects of cyclophosphamide on pulmonary thrombopoiesis in rats.

Cyclophosphamide (CP), an antineoplastic and immunosuppressive agent, even when administered in large doses, slightly affects the quantity of blood platelets. The aim of the present study was to analyse the effect of single intraperitoneal administration of CP (150 mg/kg b.w.) on the quantitative changes in platelets obtained from the left and right ventricle of the heart, as well as to evaluate the occurrence of megakaryocytes in lung tissue depending on the period of time that passed from CP administration. In control subgroups, fewer platelets were found in the blood collected from the RV compared with the left ventricle at all time intervals. After 1 and 3 days following i.p. administration of CP, a decrease was observed in the number of platelets both in the blood from the right ventricle and left ventricle when compared with control. However, after 14 days, the number of platelets in the blood from the left ventricle was higher, compared with the left ventricle and right ventricle of control animals, and significantly higher (p < 0.001747), compared with their number obtained from the right ventricle of CP-receiving animals. Simultaneous ultrastructural examinations with transmission electron microscopy revealed the increased number of platelets in the lung vascular bed of CP-receiving rats at all time intervals. However, megakaryocytes were found 7 and 14 days after administration of CP. The findings clearly indicate that the lungs could be a major place of thrombopoiesis following therapy with a single large dose of CP.

Animals↗
PubMed1998

[Chronic cor pulmonale--a disease of the right or both ventricle(s) of the heart?].

Some aspects of the development of chronic cor pulmonale have been presented. Special attention has been paid to the possibility of simultaneous formation of changes in the muscle of both ventricles of the heart and to an increase in the number of capillaries in the myocardium in the early stage of this disease. Angiogenesis, the role of hypoxia and endothelins in the pathogenesis of these changes have been discussed.

Chronic Disease↗
PubMed1998

[Diagnostic value of nucleolus organizer regions (NORs) in premalignant and malignant lesions of the cervix].

The proliferative activity of CIN and SCC lesion was examined using silver-binding argyrophilic nucleolar organizer region (AgNOR) staining. AgNORs have been counted in sections of routinely processed paraffin embedded tissue blocks. The specimens consisted punch biopsies, cones or surgery. The number of black dots (AgNORs) appearing on the nuclei were counted. Significant variation in AgNOR counts were observed between CIN III and SCC compared with the other study groups (p < 0.001). These results suggest that AgNOR counts may be of significance in the evaluation of cervical lesions and could elaborate histopathological diagnosis.

Female↗
PubMed1998

Overexpression of the nucleolar organizer regions (NORs) in the thyroid follicular tumours. Comparison of the cytological and histopathological examinations.

The aim of the study was to determine the usefulness of the expression of argyrophilic nucleolar organizer region proteins (AgNORs) as a marker of malignancy degree in thyroid follicular tumours. The study used the postoperative material of thyroid glands and cytologic material obtained with fine needle aspiration biopsy. Follicular adenoma, carcinoma and struma nodosa hyperplastica-type changes were analysed. The comparison of the AgNOR parameters examined revealed statistically significant differences between the groups of follicular carcinoma and follicular adenoma, follicular carcinoma and nodular goitre, and between adenoma and nodular goitre. However, the "overlapping" of value ranges was observed between the groups of follicular carcinoma and adenoma, as well as follicular adenoma and nodular goitre. This referred particularly to atypical adenoma parameters found within a non-diagnostic range. No differences were observed in the counts of AgNOR dots between histologic and cytologic material obtained with fine needle aspiration biopsy.

Adenocarcinoma, Follicular↗
PubMed1997

Evaluation of CA 15-3 serum levels in breast cancer patients.

The usefulness of post operative serum CA 15-3 determination was evaluated in a group of 57 breast cancer patients. CA 15-3 was assayed at baseline and every 3 months for a median follow-up of 34 months. Recurrence-free survival probability for patients with elevated serum CA 15-3 levels before surgery was 53.5% versus 88% for patients with normal serum CA 15-3 levels (p < 0.201). In 12 of the 15 patients with increased trend for CA 15-3 during follow-up, elevated CA 15-3 levels preceded (10 patients) coincided (2 patients) with the clinical detection of tumour recurrence, providing a predictive value of an increased trend of 80%. In the group of 10 patients who showed elevated serum CA 15-3 levels prior to the detection of recurrence, the median load-time was 5 months/(range 3-11 months). We concluded that CA 15-3 had prognostic value in breast cancer. Serial determinations of CA 15-3 was consistent with the treatment results.

Adult↗
PubMed1997

Contribution of capillary endothelium to cor pulmonale development in experimental lung emphysema.

The aim of this study was the ultrastructural analysis of the changes in the endothelium of myocardial capillaries and the processes observed in their vicinity in consecutive stages of experimental lung emphysema. Emphysematous changes in the lungs of Wistar rats were induced with a single intraperitoneal infusion of proteolytic enzyme solution, papain, in a dose of 20 mg/kg b.w./0.5 ml PBS. Ultrastructural analysis of the changes within the right ventricle and the subendocardial part of the left ventricle of the heart was made by a transmission electron microscope after 1, 3 and 6 months following papain administration. Special attention was paid to the analysis of changes found in the vicinity of newly formed myocardial capillaries. Animals killed after 1 and 3 months following papain infusion showed blurred borderline between the endothelium of the newly formed myocardial capillaries and perivascular connective matrix. The new vessels had poorly developed basement membrane. Fibrillary structures and/or collagen fibrils were found instead. The latter frequently demonstrated features of disorders in the structure and spatial distribution. Neither fibroblasts nor their processes were observed in the vicinity of the new collagen fibrils. Pericytes were occasionally present. In the later period (6 months from intratracheal papain infusion) well formed collagen fibres dominated in the vicinity of myocardial capillaries. The results of the ultrastructural analysis suggest the possibility of active contribution of endothelial cells to the processes of perivascular fibrosis within the myocardium in the course of experimental lung emphysema.

Animals↗
PubMed1997

Fine needle aspiration biopsy cytology of pulmonary tumors.

Transthoracic fine needle aspiration specimens of the pulmonary tumors were obtained from 144 patients. Fine needle aspiration biopsy were performed using local anaesthesia and ultrasonographic or scopie control. The 20-gauge needles were used to obtain the specimens. 93 of patients have been diagnosed as squamous cell carcinomas, 28 as adenoid carcinomas, 18 as anaplastic carcinomas and 5 as a non neoplastic lesions (tuberculoma and abscessus). The tumors were typed according to the second WHO histological classification. Analysis of the date indicated that malignant neoplasms were identified correctly with an accuracy of 92.0%. There were not false positive diagnoses. There were two false negative diagnoses (squamous cell carcinoma) and in three cases the diagnosis were as suspected for malignancy. The results confirmed the value of fine needle aspiration cytopathology for the diagnosis of pulmonary tumors.

Adenocarcinoma↗
PubMed1997

Contribution of capillary endothelium to the processes of pulmonary tissue rebuilding in the course of acute enzymatic lung injury.

Ultrastructural analysis was made of the pulmonary capillary endothelial cells, with special regard paid to their possible contribution to the processes of pulmonary tissue rebuilding initiated by intratracheal infusion of a proteolytic enzyme-papain. Experimental animals (male Wistar rats) were additionally given BCG vaccine in doses activating the alveolar macrophage system. The study was performed after 1 day, and 1, 4 and 12 weeks following papain administration. In the animals given BCG vaccine, ultrastructural exponents of endothelial cell activation were found. Cumulation of inflammatory cells, mainly monocytes, was observed in vascular lumen. Application of papain solution to animals caused a number of damaging and exudative changes, being most pronounced within vascular endothelium in animals simultaneously given BCG vaccine. In later periods, rebuilding and productive processes, accompanied by collagen cumulation in the interstitium of interalveolar septa, dominated. In the animals receiving BCG vaccine and papain, ultrastructural pictures indicated active contribution of endothelial cells to the processes of pulmonary tissue rebuilding initiated by intratracheal infusion of the proteolytic enzyme.

Animals↗
PubMed1997

Cyclophosphamide in diffuse lung damage.

Some cyclophosphamide toxic effects on lung tissue are presented. Cyclophosphamide metabolism, pathogenesis of lung damage and morphological lung tissue changes caused by that agent were characterized. Attention was focused on BAL evaluation as a useful method in the monitoring of lung tissue damage degree.

Animals↗
PubMed1997

Effects of pentoxifylline on some peripheral blood parameters and haemostasis in acute pulmonary tissue injury after cyclophosphamide.

Cyclophosphamide (CP) is one of the widely used cytostatic drugs, with a strong toxic influence on pulmonary tissue. Experimental works have shown that a single high dose of CP causes injury to all elements of the interalveolar septum, especially to the alveolar endothelial and epithelial cells. The aim of this work was to evaluate the influence of pentoxifylline (PTXF) (30 mg/kg b.w.) on the ultrastructure of lungs capillaries and blood cell count as well as plasma fibrinogen levels in Wistar rats after intraperitoneal injection of 150 mg/b.w. CP. We established that in the doses applied PTXF had no statistically significant influence on the number of leukocytes and erythrocytes determined in the left ventricular blood of rats receiving CP, while the number of white cells from animals given PTXF only was higher than in controls. There was a smaller decrease in the number of platelets (p < 0.05) and smaller reduction in fibrinogen level (p < 0.01) in the serum of PTXF-CP animals than in the CP group. The results obtained suggest a protective effect of PTXF on CP induced changes, which have been evidenced in some of the parameters examined. Ultrastructural examinations found the lungs to be the organ of extramedullary thrombocytopoiesis in CP-treated animals and revealed that platelet accumulation in the system of lung capillaries was a potential cause of the decrease observed in the number of blood platelets following CP administration.

Animals↗