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Biomedical subjects

B N Prasad

Publications and source records attributed to B N Prasad.

6 recordsLinked to original sources
PubMed1991

Purification and characterization of a neurotoxic phospholipase A2 from Indian cobra (Naja naja naja) venom.

Snake venoms contain multimolecular forms of phospholipase A2 which are diverse with respect to their pharmacological properties. A neurotoxic PLA2 from Naja naja naja venom has been purified in two steps. (1) The whole venom was fractionated on CM-Sephadex C-25 column; 4.6% of the total PLA2 activity recovered was found in the NN-V fraction. (2) The NN-Vb-PLA2 fraction was purified to homogeneity by gel filtration of fraction NN-V on Sephadex G-50. It is a basic protein with a mol. wt between 10,500 and 11,000, and is more toxic than other basic PLA2s purified from Naja naja naja venom. The LD50 of NN-Vb-PLA2 is 0.27 mg/kg body wt. It induced neurotoxic symptoms in experimental mice and is devoid of myotoxic, anticoagulant and edema-inducing activities.

Animals↗
PubMed1986

Isolation of Coxiella burnetii from human sources.

A total of 153 human milk samples were subjected to Capillary Agglutination Test (CAT) for determination of C. burnetii antibodies using phase-I CAT antigen and 22 (14.37%) samples were found to be positive. Twenty two CAT positive milk samples were further processed for the isolation of C. burnetii. Of seven samples positive in first screening, only four revealed presence of C. burnetii by giving specific seroconversion in guineapigs. Similarly, 16 aborted placentas were processed for C. burnetii and three yielded morphological evidence for rickettsial bodies on first screening. However, only one of three samples positive in first screening showed presence of C. burnetii in aborted placentas by specific seroconversion in guineapigs. Isolation of C. burnetii from aborted human placenta is the first report from India.

Agglutination Tests↗
PubMedDate not supplied

Re-expansion pulmonary edema and hypotension.

A case with diagnosis of non-Hodgkin's lymphoma and superior vena cava obstruction on chemotherapy, presented with respiratory distress and massive pleural effusion of right hemithorax. On removal of 3.5 litres of fluid, he developed pulmonary edema of the same side and hypotension.

Adult↗
PubMedDate not supplied

Massive pleural effusion, pseudocysts and pancreatico-pleural fistula with asymptomatic pancreatic disease.

A case of chronic pancreatitis with multiple pseudocysts of the pancreas and a pancreatico-pleural fistula causing massive left sided pleural effusion is reported, being a rare and an unusual cause of pleural effusion. This case illustrates the importance of pleural fluid amylase estimation in the diagnosis and the role of endoscopic retrograde cholangio pancreatography to elucidate the pathogenesis of such an effusion. The role of medical management is also emphasized.

Adult↗
PubMedDate not supplied

Recurrent pneumothorax.

A young man with spontaneous pneumothorax who suffered from fourth recurrence, is being reported. He was treated by surgical pleural abrasion.

Adult↗