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Biomedical subjects

B Nilsen

Publications and source records attributed to B Nilsen.

9 recordsLinked to original sources
PubMed2000

New methods to use fish cytochrome P4501A to assess marine organic pollutants.

A new methodology has been developed to assess cytochrome P4501A expression in two South Atlantic Spanish fish, guilthead seabream (Sparus aurata) and grey mullet (Liza aurata), used as pollution bioindicators. Degenerate oligos were used to amplify by reverse transcription and PCR (RT-PCR) specific cyp1A cDNA sequences, used subsequently to design specific primers to get the full cDNA by rapid amplification of cDNA ends. A new assay has been developed to quantitate cyp1A expression by RT-PCR in an automated DNA sequencer. The effect of beta-naphthoflavone inducing biotransformation has been used to compare three distinct pollution biomarkers: EROD activity, ELISA determination of CYP1A, and 2-aminoanthracene (2-AA) activation. Immunodetection by ELISA or Western blot was inconsistent in S. aurata and L. aurata. EROD activity yielded satisfactory results; the higher induction was observed by bioactivation of 2-AA to mutagens detected with strain BA149 of Salmonella typhimurium, in agreement with the high sensitivity previously described for this biomarker. The present paper summarizes the current status of our research.

Amino Acid Sequence↗
PubMed1996

[The recurrence frequency in inguinal hernia. A 10-year survival material].

Over a period of ten years, from 1980 to 1989, 701 hernioplasties were performed in 592 patients. 582 of these patients were available for follow-up examination (83%). The average follow-up was 10.3 years, median 10.2 years. The recurrence rate was 12%, 8% of the recurrences occurred after hernioplasties performed by specialists, and 13% after operations performed by surgeons in training. The results are discussed. The authors briefly review recent developments in hernia surgery.

Adolescent↗
PubMed1992

[Ogilvie syndrome].

Ogilvie's syndrome, or acute colonic pseudo-obstruction, is clinically impossible to differentiate from mechanical colonic ileus. Ogilvie's syndrome is associated with several conditions but the most common associated factor is surgery. Earlier, coecostomi or colostomy was the treatment of choice but most experts will agree that, although several methods of treatment have been proposed, the one now to be preferred is deflation by a colonoscope.

Aged↗
PubMedDate not supplied

A prospective comparison of duplex sonography vs angiography of the vertebral arteries.

A prospective blind comparison was completed between duplex sonography and angiography of the vertebral arteries. Thirty-two vertebral arteries were studied for direction of flow, degree of origin plaque or stenosis, Doppler characteristics, and vessel size. The vertebral arteries were reliably identified by imaging their course from the subclavian artery into the transverse foramina and by identifying a Doppler signal similar in waveform to the internal carotid artery. With Doppler, a 90% accuracy was obtained for direction of blood flow. Nonvisualization of origins was primarily due to vessel depth and/or tortuosity. In 12 vessel origins that were well seen with both techniques, angiography and sonography agreed in two-thirds of the cases. In four cases, origin plaque was underestimated with duplex imaging. Interestingly, no Doppler frequency or velocity elevation was identified distal to significant stenoses. One false-positive diagnosis of occlusion occurred with sonography, in which a 99% origin stenosis resulted in no detectable Doppler signal. By comparing sonography with angiography, sonography was shown to be 80% accurate in determining vertebral artery size. Our preliminary results indicate that duplex scanning is a reasonably accurate screening technique for size, patency, and direction of blood flow in the vertebral arteries. Duplex evaluation of the vertebral artery origin was limited by vessel depth, tortuosity, and calcifications.

Adult↗