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B Oh

Publications and source records attributed to B Oh.

31 records · Page 2Linked to original sources
PubMed1996

Rotavirus stimulates IL-8 secretion from cultured epithelial cells.

Rotavirus is the most important cause of severe gastroenteritis in children worldwide. We have investigated cytokine responses to rotavirus infection of cultured intestinal epithelial cells. Interleukin 8 (IL-8) is a chemotactic and cell-activating cytokine that is synthesized by epithelial cells and induced in response to bacterial enteric pathogens. Rotavirus inoculation increased IL-8 mRNA levels in cultured intestinal epithelial cells within 2 hr of infection. IL-8 secretion increased 10(2)- to 10(3)-fold by 8 hr postinfection. Secretion of TNF alpha or IL-1 beta, cytokines which themselves increase IL-8 secretion, was not induced by rotavirus, nor was that of TNF alpha, IFN alpha, IFN gamma, or IL-6. Neutralizing antibodies to TNF alpha or IL-1 alpha/beta did not affect the IL-8 response. Secretion of IL-8 was dependent on an intact viral capsid, as single-shell particles were inert. Neutralizing monoclonal antibodies (vp7-specific) that do not block cell attachment did block rotavirus stimulation of IL-8 secretion, indicating that attachment to the cell surface is not a sufficient stimulus to induce IL-8. Genetically inactivated rotavirus was also effective for IL-8 induction, indicating that viral replication was not required. These data suggest that epithelial cytokine IL-8 may be an important mediator of the host response to viral gastroenteritis pathogens such as rotavirus.

Animals↗
PubMed1996

Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3.

To study the molecular function of genes expressed during preimplantation development, we isolated a novel maternal transcript SSEC (Stage Specific Embryonic cDNA)-26 from a partial subtraction library of mouse unfertilized eggs and preimplantation embryos. The SSEC-26 transcript is abundant in the unfertilized egg and also actively transcribed from the newly formed zygotic genome. On the basis of its expression in eggs and embryos, this new mouse gene is named Mem (maternal-embryonic) 3. The genomic locus of Mem3 has been mapped to Chromosome (Chr) 8 near the D8Mit78 marker and the glutaryl CoA dehydrogenase (Gcdh) locus. The deduced amino acid sequence of MEM3 resembles that of the yeast VPS (Vacuolar Protein Sorting) 35 in two separate domains. A cDNA sequence of the potential human homolog of Mem3 has been assembled with partial clones from the EST database and assigned to human Chr 16.

Amino Acid Sequence↗
PubMed1990

Alternative splice forms of the murine T-cell receptor beta transcript in a cytotoxic T-cell line.

The processing of the primary transcript of the murine T cell receptor c beta region can result in two distinct forms of protein. The optional exon, C beta O, is appended at the 5' end of the constant region and to date has been observed at low frequencies in cDNA clones from heterogeneous populations of T lymphocyte. Because individual cell lines were not analyzed in those studies, it was not known whether small numbers of T lymphocytes use the C beta O exon exclusively or instead most T-lymphocytes use the C beta O exon but at low levels. We have determined that our CTL clone, B6.cl 4, produces both C beta O+ and C beta O- functional RNA transcripts. To determine the C beta O usage in this CTL clone, we coupled cDNA synthesis with the polymerase chain reaction using oligonucleotides corresponding to sequences at the V beta 14 region and sequences corresponding to C beta O or C beta 1. The data indicate that B6.cl 4 CTL clone is able to use either splice site but uses the C beta O exon at a frequency of approximately 2-3%.

Animals↗