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Biomedical subjects

B Panaszek

Publications and source records attributed to B Panaszek.

At least 19 recordsLinked to original sources
PubMed1994

Specific pollen allergen activates eosinophils of the patient with chronic allergic contact urticaria.

The aim of the study was to evaluate the activation of eosinophils in an unique case of a young man with atopy manifested as chronic pollen contact urticaria. In order to reveal the role of eosinophils in that case, the study was performed by means of monoclonal antibodies EG2 and chemiluminescence. In addition, comparative electron microscopic study of peripheral blood and skin infiltrating eosinophils were performed for which the name ultrastructural morphometric analysis of intracytoplasmic eosinophil granules has been proposed. The results indicated, that 40% of peripheral blood eosinophils were activated spontaneously and they were more active than those in skin infiltrates. Specific pollen allergen caused activation of 100% of peripheral blood eosinophils. The study suggests presence of a systemic pattern of eosinophil activation in atopy.

Adolescent↗
PubMed1980

Effect of histamine on the spontaneous rosette formation.

Eight persons with clinically diagnosed hay fever were examined. The control group consisted of ten subjects without any signs of allergy. T lymphocyte subpopulation was evaluated by E rosette test. The effect of histamine was studied adding various concentrations of histamine (from 10(-9) to 10(-4) M) to the suspension of lymphocytes. A proportional drop in the percentage of the formed E rosettes could be seen at histamine concentrations ranging between 2 x 10(-9) and 2 x 10(-6) M. At 10(-6) up to 10(-4) M there was both an increase and further reduction in the percentage of the E rosette formation. A question arises whether there exists any correlation between the decreased number of T lymphocytes and elevated content of histamine in atopic persons?

Adult↗
PubMed1980

Effect of histamine on the spontaneous E rosette formation after blockade of histamine H2 receptors with cimetidine.

Thirteen patients with clinical diagnosis of hay fever, were examined. T lymphocytes subpopulation was determined by the E rosette test. The present study is concerned with the effect of histamine on the ability of T lymphocytes for E rosette formation after blockade of the histamine H2 receptor with its inhibitor cimetidine. It has been found that histamine effect is expressed by the histamine H2 receptor, as its blockade with cimetidine leads to the failure of histamine to inhibit rosette formation by T lymphocytes.

Adolescent↗
PubMed1980

T and B lymphocytes in patients with hay fever.

In 20 patients with hay fever and 19 control subjects the rosette formation test was carried out to determine T and B lymphocyte subpopulations. T and B lymphocyte subpopulations are expressed by the percentage of E, EA and EAC rosettes, respectively. Statistical analysis revealed a significant reduction in the number of E rosettes (p < 0.001), and a slight, but statistically significant decrease in the number of EAC rosettes (p < 0.05) in hay fever patients.

Adolescent↗
PubMed1980

Effect of isoproterenol and propranolol on the mast cell degranulation and on the passive cutaneous anaphylaxis in mice.

Effect of isoproterenol and propranolol on the production of hemocytotropic antibodies and degranulation of peritoneal mast cells from sensitized mice was studied. It was demonstrated that stimulation with large doses of the antigen caused degranulation of peritoneal mast cells and that additional administration of a beta antagonist propranolol failed to increase the degranulation phenomenon. Marked inhibition in degranulation of mast cells was seen in mice sensitized with large and small doses of antigen after isoproterenol. In 2h PCA test in animals immunized with large doses of the antigen a higher activity of PCA reaction illustrating the concentration of IgG1 antibodies was noted. With higher doses of the antigen and propranolol the titers of the antibodies were markedly higher. The inhibiting effect of isoprenaline on the passive cutaneous anaphylaxis was observed only in the 72 h-test, mostly in the group of mice immunized with small doses of the antigen.

Animals↗