PubMed Health⌕ Search

Biomedical subjects

B Perissel

Publications and source records attributed to B Perissel.

At least 19 recordsLinked to original sources
PubMed1992

Chromosome analysis by image processing in a computerized environment. Clinical applications.

Dealing with a routine regional cytogenetic activity, we have developed and adapted to clinical work a semi automatic karyotyping machine. Attempts for an accurate automated chromosome classification using a neural network have led to partial results. A specific adaptation to cancer cytogenetics is under development (determination of the modal number, translocations analysis with densitometric curves, automatic identification of markers). A specific program allows quantification of chromosome labelling with radioactive probes. Exchanges of digitized karyotypes are feasible with labs using automated karyotyping machines. A local network connects several karyotyping and metaphase finding stations. Guidelines for an international data bank concerning abnormal chromosome images have been elaborated. On the other hand the ISH techniques have been applied to the following topics: identification of human chromosome aberrations in amniotic and chorionic cells, chromosome studies of human gametes and embryos (including sex determination), identification of markers in cancer cells.

Chromosome Aberrations↗
PubMed1989

[Chromosome analysis using image processing. Recent aspects and perspectives].

After a brief description of the automatic metaphase finding and karyotyping systems actually available, the authors describe an interactive method for chromosome analysis. The edges of each chromosome are delineated automatically. The use of 256 grey levels and 512 x 512 pixels allows the accurate classification. The result may be recorded on hard copy, videotape or disk. Present improvements of the Chromoscan include histograms, quantitative studies and the use of an expert system.

Chromosome Aberrations↗
PubMed1987

[A new system of chromosome analysis by image treatment: the chromoscan].

The authors present a new interactive system for chromosome analysis. All the operations are checked by the biologist with the use of a "mouse". This system avoids the photographic work and the manual classification. Its main interest is to carry out quickly the karyotype after the slide preparation.

Animals↗
PubMed1986

Translocation t(3;20) associated with thrombocythemia in Ph-positive CML.

A patient with Philadelphia (Ph) chromosome positive chronic myelocytic leukemia is described who also developed an abnormality of chromosome #3, i.e., t(3;20)(p21;p13), in blast crisis. This abnormality may be connected with the advent thrombocythemia. The disease was a thrombopenia in the initial phase.

Adult↗
PubMed1985

[In vitro study of fibroblasts from patients with Duchenne muscular dystrophy. Evaluation of capping and ultrastructural aspect].

There have been conflicting studies of lymphocyte capping from patients with Duchenne Muscular Dystrophy. We have evaluated the proportion of capped fibroblasts of 10 patients with Duchenne Muscular Dystrophy. The results, compared with 15 normal controls, showed that the reduction of fibroblast capping is correlated to age patients. Additional studies, decreased number of retracted fibroblasts after colchicine incubation and ultrastructural observations, suggest that capping deficit would be the consequence of microtubular system alteration. This late phenomenon may be secondary to the primary membrane defect.

Adolescent↗
PubMed1983

[Trisomy of the short arm of 9 with isochromosome 9p and partial monosomy Yq].

A patient with trisomy 9p in association with monosomy of the heterochromatic distal portion of the Y chromosome is reported. The rearrangement is probably due to malsegregation of a translocation 9p, Y and formation of an iso (9p). The phenotype of the patient is characteristic of trisomy 9p. There is a significant increase of GALT activity.

Abnormalities, Multiple↗
PubMed1979

[Action of erucic and palmitic acids on rat cardiac myoblasts in primary cell culture. An ultrastructural study (author's transl)].

Primary cultures of beating myocardial cells of neonatal rat are taken in order to observe the ultrastructural modifications caused by certain long chain fatty acids (erucic acid C22 : 1 and palmitic acid C16 : 0). Reference cultures are established and observed at the same time as the others. The eurcic acid create an intense steatosis, on the opposite palmitic acid does not. On the contrary the transormations of certain cellular organites such as mitochondria, dictyosomes, rough endoplasmic reticulum and ribosomes are observed in both cases.

Animals↗
PubMed1976

Differentiation of the plasma membrane of hepatic cells in monolayer cultures.

Hepatocytes from rats were isolated by treatment with trypsin and cultured. Plasma membranes at different culture stages were observed by electron microscopy. The activities of 5' nucleotidase and adenosinetriphosphatase on the plasma membranes were examined. The cell coat was also studied by use of the concanavalin A-peroxidase technique. The surfaces of single cells, covered with microvilli, are the site of adenosinetriphosphatase activity only and are devoid of 5'-nucleotidase activity. After a few h of culture, the cells are grouped together in tight clusters or long trails and are separated by an intercellular space of 250 A, partially permeable to lanthanum nitrate. The juxtaposed plasma membranes on which 5'-nucleotidase and adenosinetriphosphatase activities occur also delimit spaces similar to bile canaliculi. The formation of junction complexes and their permeability to lanthanum nitrate was also studied. No enzymatic activity is observed at the junctions. The numerous tight junctions, impervious to the tracer, are always accompanied by a profusion of microfilaments. Mature desmosomes are rare, and are present only in the form of "maculae adhaerentes diminutae." The gap junctions, nearly always permeable to the tracer, form rapidly and assume a variety of shapes (trail, bulge and ring-like), the significance of which is open to discussion. The use of concanavalin A permits localization of the free sugar sites on the surface of the cells, in the pinocytotic vesicles and in the internal space of the gap junctions.

Adenosine Triphosphatases↗