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Biomedical subjects

B Popova

Publications and source records attributed to B Popova.

7 recordsLinked to original sources
PubMed1995

Transcriptional activation by DNA-binding derivatives of HSV-1 VP16 that lack the carboxyl-terminal acidic activation domain.

The herpes simplex virus transactivator VP16 directs the assembly of a multicomponent protein-DNA complex with cellular components Oct-1 and VCAF-1, contributing a potent carboxyl-terminal acidic activation domain that is essential for activation of gene expression in mammalian cells. We show here that VP16, devoid of this acidic activation domain, functions as a strong transcriptional activator in the yeast Saccharomyces cerevisiae when appended onto a heterologous GAL4 DNA binding domain, as determined by measuring activation of a resident GAL1:lacZ reporter gene. Deletion analysis indicated that sequences contained within the amino-terminal 369 amino acids of VP16 were necessary for transactivation by truncated VP16. Activation by truncated VP16 in yeast was comparable to that observed with a hybrid protein consisting of the GAL4 DNA binding domain linked to the VP16 acidic activation domain. Similar GAL4-VP16 hybrid proteins were only marginally active in mammalian cells. Sequence requirements for transactivation by truncated VP16 can be demarcated from domains of VP16 that are required for interaction with VCAF-1 and for protein-DNA complex formation with Oct-1. Our findings indicate that VP16 contains additional sequences upstream of the acidic activation domain that may play a direct role in transactivation.

Animals↗
PubMed1994

Herpes simplex virus VP16 forms a complex with the virion host shutoff protein vhs.

Herpes simplex virus (HSV) virions contain at least two regulatory proteins that modulate gene expression in infected cells: the transcriptional activator VP16 and the virion host shutoff protein vhs. VP16 stimulates transcription of the HSV immediate-early genes, and vhs suppresses host protein synthesis and induces accelerated turnover of cellular and viral mRNAs. We report here that vhs binds directly to VP16: vhs and VP16 were coprecipitated from infected cells by an anti-vhs antiserum, and vhs and VP16 protein A fusions each bound intact versions of the other protein in a solid-phase capture assay. In addition, vhs and VP16 interacted in the two-hybrid activator system when coexpressed in Saccharomyces cerevisiae. vhs residues 238 to 344 were sufficient for the interaction, and the VP16 acidic transcriptional activation domain was not required. vhs blocked the ability of VP16 to enter a multiprotein complex on an immediate-early TAATGARATTC consensus sequence, indicating that vhs interacts with one or more regions of VP16 required for promoter recognition. We suggest that this interaction may play a structural role in the assembly of HSV virions and modulate the activity of vhs during infection.

Animals↗
PubMed1986

[Our results with the secondary prevention of myocardial infarct].

The communication summed up the results from the two prospective follow-up care-prophylactic studies on patients with a past history of acute myocardial infarction (AMI). The first study covers all patients, with a past history of AMI and discharged in 1963, 1964, 1966 and 1967, a total of 378, with a follow-up period from 6 to 11 years. The direct task of the follow up was the prevention of a repeated infarction. The prophylactic measures, carried out with the followed-up patients were: control of body mass by a hypocaloric and antisclerotic diet, sufficient physical activity and medicamentous treatment when needed. Two groups of patients were differentiated: experimental (the patients adhering to the instructions prescribed) an control (patients that did not adhere to the recommendations for correction of body mass with no motor regimen). After the termination of the observation it was established that 12.6% from the experimental group had repeated infarction and 28.3%--in the control group. The lethality in the experimental group was 21.7% and in the control--43.9%. The second prospective observation covered 250 patients (reduced to 241 in time), with a past history of AMI and discharged within the year 1975. The follow up lasted for 5 years under the same conditions of follow-up care. Summing up our 5-year secondary prophylaxis carried out mainly with a diet, physical activity and partially with antiaggregants and medicamentous treatment when needed, we could ascertain and draw the conclusions.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1984

[Work capacity and motor activity of patients with a history of myocardial infarct].

A total of 241 patients with a past history of AMI were followed up with a view to the restoration of their capacity for work and its dependence on their motor activity. All deceased, 6 months after the acute infarction, were also included in the group. Work was resumed by 43,7 per cent from the whole group. But the percentage of the patients with preserved capacity for work to the age of 60 that had outlived the 5-year observation period is 75; 63, out of 84 subjects at that age, began work within an average period of 8 months after the onset of the infarction. The periods till the beginning work of our patients are longer as compared with the periods reported by foreign authors. An obvious correlation was established between the patients' capacity for work, with a past history of AMI, and their motor activity.

Adult↗
PubMed1983

[Survival of patients who have had a myocardial infarct as a function of various risk factors].

Survival was studied among 250 patients with a past history of acute myocardial infarction (AMI), followed up for 5 years. A total survival of 55,6 per cent was established. The factors affecting the survival are: age, arterial hypertension, diabetes mellitus, sex, rhythm and conduction disorders, restricted physical activity, decompensation signs after AMI, increased measurements of the heart, repeated infarction as well as the presence of anginose complaints postinfarction period. The patients, with a past history of HMI should be subjected to effective follow-up care with a view to the campaign against the factors, threatening life.

Adult↗