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Biomedical subjects

B Presentey

Publications and source records attributed to B Presentey.

18 recordsLinked to original sources
PubMed1997

Hepatozoon canis infection in a litter of Dalmatian dogs.

Infection with Hepatozoon canis is described in a litter of seven Dalmatians. Four littermates were presented with concurrent hepatozoonosis and parvoviral enteritis and the remaining three puppies were parasitemic with H. canis with no other concurrent disease. Parasitemia ranged between 3% and 67% of the blood neutrophils. The mean number of parasitized neutrophils per microliter among littermates with concurrent hepatozoonosis and parvoviral enteritis was 1139 (+/-447 SD) on the day of admission, compared with 470 (+/-379) among littermates with hepatozoonosis only. Puppies with hepatozoonosis and parvovirus infection at admission differed significantly in their degree of H. canis parasitemia from their littermates which were not infected with parvovirus (P = 0.0286). Concurrent parvoviral enteritis and hepatozoonosis in the dog are reported here for the first time.

Animals↗
PubMed1990

[Alder anomaly accompanied by myeloperoxidase mutation].

A third form of the Alder anomaly is described in which the peroxidase of neutrophilic polymorphonuclear cells (PNC) is resistant to fixation in methanol, a necessary step in the May-Grunwald Giemsa staining procedure. This property of the abnormal cells is similar to that of eosinophils, rather than normal PNC. However, the PNC in this form of the condition also have the abnormal granulation typical of the other forms of the anomaly. It is suggested that in this third form, 2 genetic aberrations are involved, 1 affecting the formation of the PNC granules and the other a gene mutation which encodes for the production of myeloperoxidase in those cells.

Hematologic Diseases↗
PubMed1986

Alder anomaly accompanied by a mutation of the myeloperoxidase structural gene.

In this paper, a third form of Alder anomaly is described. In addition to the morphological changes which are characteristic of this anomaly, the peroxidase of the neutrophil polymorphonuclear leukocytes (NPNL) is abnormal in its properties. It is resistant to the fixation step in the May-Grünwald Giemsa procedure and is similar, therefore, to the eosinophil peroxidase. In the phenotype observed, 2 genetic changes are probably involved: one is a change in the regulation of NPNL granulation, the other is a mutation in the myeloperoxidase structural gene. In the light of these new findings, 3 forms of the anomaly are now known: the original Alder anomaly, in which the abnormal granulation, particularly in the granulocytes, is not linked with any hereditary disorder; the Alder-Reilly form, in which in addition to the abnormal granulation the leukocytes also contain inclusion bodies, the phenomenon sometimes being associated with mucopolysaccharidosis (gargoylism), and the third form of anomaly described here, where-apart from the abnormal granulation-the myeloperoxidase behaves differently, resembling that of eosinophils in its resistance to methanol fixation.

Cytoplasmic Granules↗
PubMed1984

Ultrastructure of human eosinophils genetically lacking peroxidase.

The structure of human eosinophils genetically lacking peroxidase is described. Structural changes were discerned in the specific granules of the deficient eosinophils as compared to normal eosinophils. Apart from the inhibition of peroxidase formation, this mutation also affects the structure of the specific granules - primarily the interrelationship between the matrix and the core.

Cytoplasmic Granules↗
PubMed1980

Genesis, ultrastructure and cytochemical study of the cat eosinophil.

Eosinophils from cat bone marrow and peripheral blood were studied by electron microscopy and cytochemical procedures. The maturation of eosinophils and formation of typical granules were described. Contrary to the accepted opinion that the core of animal's eosinophilic specific granules have a crytal-like structure, our observations revealed that the core has a myelin-like cylindrical appearance, whose layered formation proceeds from the inside outwards. Electron microscopic observations revealed that localization of reaction product to potassium pyroantimonate and phosphotungstic acid and to acid phosphatase activity was similar to that of eosinophils of man and other animals. Antimonate deposits and acid phosphatase activity were detected between the layers of the myelin-like structure of the core. Eosinophil granules failed to yield a positive reaction for peroxidase activity. The secretory activity of the eosinophil is discussed.

Acid Phosphatase↗
PubMed1979

Eosinophilic leukaemia: morphological, cytochemical, and electron microscopic studies.

The eosinophils of a patient with eosinophilic leukaemia were studied with 13 different cytochemical methods using light and electron microscopy. Apart from the 'left shift' of the eosinophils in bone marrow and peripheral blood, the following morphological changes were noted: uncoordinated maturation of the nucleus and cytoplasm, changes in size of the specific granules, and hypogranulation to such an extent that some of the cells bore only very few granules. The cytochemical studies showed a strongly positive periodic acid Schiff reaction in the eosinophils, caused by a high content of glycogen, and a relatively strong positive acid-phosphatase reaction. These cells were also tested for aryl sulphatase and coenzyme Q. Electron microscopy confirmed the presence of a high-content glycogen and a strong acid phosphatase response in the cells. Peroxidase reaction, detected in electron microscopy as well, enabled us to trace the maturation of the eosinophil cell line.

Acid Phosphatase↗