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Biomedical subjects

B Puppe

Publications and source records attributed to B Puppe.

At least 19 recordsLinked to original sources
PubMed2007

Altered vocalization rate during the estrous cycle in dairy cattle.

Correct detection of estrus is a problem in dairy herds. In practice, several procedures exist for detection of estrus besides conventional visual observation by humans. These procedures deliver very different results regarding detection of estrus. It is known that the calls of female mammals can contain information about reproductive status. It is also suspected that the vocalizations of cattle contain information about age, sex, dominance status, and stage in the estrous cycle. In the present study, a methodology for the continuous automatic recording of vocalization of heifers during the periestrous period is presented. It was shown in 10 tethered heifers that the estrous climax results in an increase in vocalization rate. Vocalization rate of heifers increased approximately 84% from d -2 to 0 (related to observed estrus) and approximately 59% from d -1 to d 0. After d 0, vocalization rate decreased about 79%. Increased vocalization was correlated with the visual observation of estrus by humans. We also found 2 different structures in the vocalization of heifers. The harmonic structure showed regular frequency bands, whereas the nonharmonic structure was noisy. The hypothesis that the disharmonic structure increases near the estrous climax was confirmed. Hence, it seems possible to get information about stage of the estrous cycle of dairy cattle by means of monitoring vocalization. The presented method of automatically detecting the rate of cattle vocalization (patent pending) could be used solely or in combination with other automated systems for detecting estrus and could considerably increase current estrus detection rates once its applicability can be demonstrated in nontethered cattle.

Animals↗
PubMed2006

Effects of attention and rewarded activity on immune parameters and wound healing in pigs.

Challenging animals with a demanding situation they are able to cope with and where they are rewarded may be a source of positive emotions and possibly of increased welfare. In order to test if this results in changes of immunological parameters and wound healing, 56 pigs (7-20 weeks of age), housed in groups of 8 animals each, were successfully trained to recognize and localize an individual acoustic summons and to receive a small portion of feed as a reward. Immune reactions and the development of a standardized biopsy wound were compared to values of conventionally fed control groups of equal size and animal-to-feeding-place ratio (2:1). In the experimental animals a significantly higher concentration of IgG as well as an increased in vitro T-cell proliferation to ConA but a reduced LPS-induced proliferation of B-cells was found, while basal salivary cortisol concentrations were similar. Wound development was better in the experimental animals as measured by the area of the inflammatory corona. It decreased more rapidly in the experimental animals since the 5th day after biopsy and was significantly smaller than in the control groups. We conclude that environmental enrichment by equipment provoking attention and cognitive activity which is rewarded by feed may play a beneficial role for physical welfare of intensively housed pigs.

Animal Feed↗
PubMed2005

[Reaction of muscles to a stimulating environment--effects on the loin muscle (M. longissimus) of Landrace pigs].

The effects of an enriched environment on growth, meat quality, and muscle biological traits were investigated using 64 castrated pigs (age range 7 to 20 weeks). Positive emotional appraisal was induced by a system that acoustically frequently called individuals out of a group (n=32) to a feeding station, where they were rewarded with small portions of feed. The results were compared with traditional fed pigs (Control; n = 32). There was no environmental effect on live weight and lean muscle percentage. However, the stimulating keeping regime reduced the intramuscular fat content of the M. longissimus by 0.2 % and increased the protein content by 0.5 %. Moreover, the drip loss of M. longissimus was 1.1 % lower in the treatment group. The analysis of muscle fibre traits (biopsy samples of M. longissimus) showed a 7 % higher proportion of oxidative muscle fibres in the pigs reared in the enriched environment. This fibre type showed a tendency to increased fibre areas. These findings indicate a higher oxidative capacity of the muscle. However, the activity of the inarker enzyme citrate synthase (CS) was not influenced and the activity of lactate dehydrogenase (LDH) was even increased compared to the control animals.

Adaptation, Physiological↗
PubMed2003

[Effects of muscle needle biopsy on parameters of humoral and cellular immunity in pigs].

The shot biopsy is a common experimental technique for the collection of samples to investigate muscle tissue characteristics or to determine meat quality features in pigs. Its application seems to be also possible in interdisciplinary research projects investigating animal stress, behaviour, and welfare. The present study on 12 group-housed pigs (age: 12 weeks, weight: 29.3 kg) shows the influence of this wound-causing technique on different humoral and cell-mediated parameters of the immune system at 1, 3, 5, and 9 days after biopsy compared to the initially investigated levels before. An enhancement of the blood sedimentation rate and both the IgG and the cellular immune response in vivo (leukocytes, lymphocytes) as well as in vitro (ConA) was observed. Furthermore, there were signs of a secondary wound infection 5 days after biopsies were taken possibly caused by mutual oral manipulation of the animals. We conclude that the muscle shot biopsy technique can be used on group-housed pigs as a method to investigate muscle physiological characteristics. The technique, however, induces immunological reactions which may interfere with stress-induced immune reactions.

Animals↗
PubMed2001

Linear prediction coding analysis and self-organizing feature map as tools to classify stress calls of domestic pigs (Sus scrofa).

It is assumed that calls may give information about the inner (emotional) state of an animal. Hence, in the last years sound analysis has become an increasingly important tool for the interpretation of the behavior, the health condition, and the well-being of animals. A procedure was developed that allows the characterization, classification, and visualization of the cluster structures of stress calls of domestic pigs (Sus scrofa). Based on the acoustic model of the sound production the extraction of features from calls was performed with linear prediction coding (LPC). A vector-based self-organizing neuronal network was trained with the determined LPC coefficients, resulting in a feature map. The cluster structure of the calls was then visualized with a unified matrix and the neurons were labeled for their input origin. The basic applicability of the procedure was tested by using two examples which were of special interest for a possible evaluation of the normal farming practice. The procedure worked well both in discriminating individual piglets by their scream characteristics and in classifying pig stress calls vs other calls and noise occurring under normal farming conditions.

Acoustics↗
PubMed2000

Early identification of neonates at risk: traits of newborn piglets with respect to survival.

Despite technological changes and improved management, piglet mortality remains a problem for both production and welfare. Most preweaning mortality occurs within the first 3 days after birth because of problems with adaptation and development. Thus, the purpose of our study was to determine the physiologic state of newborn pigs with respect to piglet survival. Data were collected from 1024 live-born piglets of 106 primiparous German Landrace sows to analyze relationships between farrowing traits, early postnatal vitality and blood chemistry, including immunity of piglets at birth. Surviving piglets were compared with those that died during the first 10 days of life. The survivors were significantly heavier at birth (P=0.001), were born earlier in the birth order (P=0.04), reached the udder and took in first colostral milk more quickly (P=0.001) and had a smaller drop in rectal temperature I h after birth (P=0.001) than dead. However, dead piglets had significantly higher blood levels of inorganic phosphorus (P=0.0001), calcium (P=0.04) and urea (P=0.05), but a lower concentration of alpha2-macroglobulin and lower lymphocyte proliferation indices in response to pokeweed mitogen (P=0.05). Models fitted for discrimination between survivors and piglets that died included, in addition to birth weight and litter size, the foraging behavior of neonates (time from birth to first suckle) and their thermoregulatory capacity (rectal temperature 1 h after birth) in the first experimental unit, as well as prenursing biochemical measures (inorganic phosphorus, calcium and glucose) in the second experimental unit. These ethophysiological and biochemical traits of early postnatal vitality are important determinants of maturity and development at birth. Hence, breeding programs and perinatal housing and feeding conditions should ensure a high physiological maturity to improve mortality rates of neonates.

Animal Husbandry↗
PubMed1999

Effects of dominance and familiarity on behaviour and plasma stress hormones in growing pigs during social confrontation.

Behavioural patterns and plasma adrenaline, noradrenaline and cortisol responses were studied in domestic pigs with different dominance status during 10-h social confrontation tests with a familiar and an unfamiliar group. Eight trials were carried out, where in each trial two groups of nine growing pigs (12 weeks old) were randomly formed. The pigs with the highest (HR) and lowest (LR) rank from each group were selected as test animals, provided with surgically implanted catheters and kept in single housing for 2 to 3 weeks. After this period, each test animal was introduced once into the familiar and once into the unfamiliar group for 10 h. Introduction of the test animals into the groups caused frequent agonistic interactions during the first 30 min and significantly more agonistic interactions during the confrontation with the unfamiliar group. The agonistic behaviour was accompanied by a rapid increase of plasma catecholamines and cortisol. HR pigs showed significantly higher plasma catecholamine concentrations and more agonistic interactions during the first 30 min compared with the LR pigs. During confrontation with the unfamiliar group, HR pigs experienced more defeats and showed a higher increase of plasma cortisol levels than during the confrontation with the familiar group. No influences of rank or familiarity were found on the other behavioural patterns examined. The results show that agonistic behaviour and activation of the sympatho-adrenomedullary and the hypothalamic-pituitary-adrenal system in pigs during a social confrontation test are determined by the former dominance rank of the animals and the familiarity of the group.

Animals↗
PubMed1999

Monitoring of piglets' open field activity and choice behaviour during the replay of maternal vocalization: a comparison between Observer and PID technique.

The paper presents a new system for the automatic monitoring of open field activity and choice behaviour of medium-sized animals. Passive infrared motion detectors (PID) were linked on-line via a digital I/O interface to a personal computer provided with self-developed analysis software based on LabVIEW (PID technique). The set up was used for testing 18 one-week-old piglets (Sus scrofa) for their approach to their mother's nursing vocalization replayed through loudspeakers. The results were validated by comparison with a conventional Observer technique, a computer-aided direct observation. In most of the cases, no differences were seen between the Observer and PID technique regarding the percentage of stay in previously defined open field segments, the locomotor open field activity, and the choice behaviour. The results revealed that piglets are clearly attracted by their mother's nursing vocalization. The monitoring system presented in this study is thus suitable for detailed behavioural investigations of individual acoustic recognition. In general, the PID technique is a useful tool for research into the behaviour of individual animals in a restricted open field which does not rely on subjective analysis by a human observer.

Animals↗
PubMed1998

Effects of social status after mixing on immune, metabolic, and endocrine responses in pigs.

The effects of social rank on immune, metabolic, and endocrine responses were studied in 10 newly mixed groups of German Landrace pigs (9 individuals each) at an age of 12 weeks. Immediately after mixing, the agonistic interactions (AI) of all group members were continuously recorded over 3 days (10 h daily). An individual dominance value (DV) was calculated by the number of wins minus defeats in relation to all decisive fights (DV < or = 0, subordinate; DV > 0, dominant). Blood samples were taken 24 h before and 3 days after mixing. The data showed that the social status had a significant effect on lymphocyte proliferation in responses to different mitogens: socially dominant pigs had higher proliferative response than subordinate pigs. In addition, during the observation period the lymphocyte activation by mitogens increased in the dominant animals and decreased in the subordinate animals with increasing number of agonistic interactions. The rise in total serum IgG concentration 3 days after mixing was higher in dominant pigs compared with subordinates. The dominance status did not significantly affect plasma metabolic levels nor cortisol concentrations. However, mixing appeared to increase glucose and total protein values and to decrease alkaline phosphatase and cortisol levels in both, dominant and subordinate pigs. In conclusion, mitogen induced cell proliferation seems to be a valuable marker for acute social stress in pigs.

Agonistic Behavior↗
PubMed1995

Evaluating four diagnostic methods with acute abdominal pain cases.

Contemporary work in medical decision support is characterized by a multitude of methods. To investigate their relative strengths and weaknesses, we built four diagnostic expert systems based on different methods (Bayes, case-based classification, heuristic classification) for analysis of the same set of 1254 cases of acute abdominal pain previously documented in a prospective multicenter study. The results of the comparative evaluation indicate that differences in overall performance are relatively small (statistically not significant). The performance depends more on the quality of the knowledge base and the case data than on the inference methods of the expert systems. Methods relying exclusively on empirical knowledge (Bayes, case-based classification) tend to have slightly higher overall performance scores due to a diagnostic bias toward ordinary and common diseases. By contrast, methods operating with expert knowledge (e.g., heuristic classification) perform slightly worse overall, but are more sensitive toward uncommon (serious) diseases.

Abdominal Pain↗
PubMed1995

[Some theoretical aspects of behavior research in domestic animals].

During the past decade, applied farm animal ethology has become of increasing significance within the behavioural sciences. The present paper investigates some of the most important terms and concepts of basic behavioural research in order to relate these approaches to farm animal ethology. It is advocated here that deviations of theoretical frameworks used in basic and applied research should be kept at a minimum, unless necessitated by particular aspects of applied research goals. It is further argued that environmental requirements of the animals and motivational causes of behaviour could be assessed more appropriately when considering behaviour as an interaction between organism and environment shaped by natural and artificial selection, i.e. as a means to ultimately maximize biological fitness. It is concluded that ultimate causation of behaviour should become a major focus in applied ethology in order to reach at valid approaches to animal welfare and improved husbandry systems which are more in line with the actual needs of the animal.

Animal Husbandry↗
PubMed1995

[The suckling behavior and development of biochemical parameters in piglets from conditions of group housing of suckling sows: preliminary results].

The suckling behaviour, the development of daily weight gains and biochemical blood parameters (immunoglobulin G, total protein, glucose, urea) were investigated during the suckling period (35 days) of piglets (Sus scrofa) in a group housing system for nursing sows (group P: 4 primiparous sows, 34 piglets; group M: 4 multiparous sows, 43 piglets). Following an imprinting period of 10 days, piglets were given the opportunity to leave the farrowing pen and to interact with the other sows (variant e: suckling bouts at own mother only, variant f: suckling bouts also at one or more unfamiliar sows). Although a number of piglets suckled at unfamiliar sows (Pf = 73.5%; Mf = 32.5%; p = 0.01), only those piglets that stayed with their own mother after mixing had some "physiological advantages" (Pe: daily weight gain from day 10 to 35, p = 0.05; Me: IgG, p = 0.05). Piglets of primiparous sows had a lower teat pair fidelity within the imprinting period (day 1 to 10). During this time, piglets of both groups suckling at unfamiliar sows showed a lower teat pair fidelity (P: ns; M: p = 0.05), tended to prefer the posterior part of the udder, and their blood glucose and urea contents were higher (P: ns; M: p = 0.05). This was interpreted as an indication of competitive strain which obviously encouraged the piglets to leave their mothers. It is necessary to carry out further systematical studies on sociophysiological mechanisms.

Animals↗
PubMed1995

[Correlations between the vitality of newborn piglets, teat order, mortality, and live weight development up to weaning].

Investigations with 693 piglets have shown, that suckling piglets with preference of cranial teat pairs were significantly superior to their littermates in all parameters of early postnatal vitality: they stood up and took in first colostrum earlier and showed a lower postnatal drop in rectal temperature in comparison with their siblings. Piglets in cranial teat position had the highest daily gain (205 g, p < 0.05) during suckling period. Highly significant influences of vitality of piglets post natum on live weight development and mortality up to 28th day of age were shown. The faster piglets took in colostrum after birth and the lower postnatal drop in rectal temperature was, the higher was the obtained daily gain during suckling period. Piglets which died within the first 7 or 28 days of age had needed a significantly longer time till first postnatal colostrum intake and a more pronounced drop in rectal temperature in comparison with their littermates.

Aging↗
PubMed1994

[The effect of birth weight on the early postnatal vitality of piglets].

Investigations with 1248 newborn piglets in 7 farms showed a high significant influence of birth weight on parameters of early postnatal vitality. The duration between birth and first standing up was by two times, the time between birth and first udder contact by 3.5 times and the duration between birth and first colostrum intake was by 4 times longer in piglets with a low birth weight (< 800 g) in comparison with heavier piglets at birth (> 2200 g). The drop in rectal temperature up to 30 minutes after birth reached 4.5 Kelvin in lightweight piglets, whereas their litter mates with a high body weight at birth had a value of 0.85 K (p < 0.01). The birth weight of piglets belongs to the most important endogenous factors with influence on the vitality of newborn piglets and has a high prognostic value in relation to the risk of losses and the live weight development of neonates.

Animals↗
PubMed1985

MED1: an intelligent computer program for thoracic pain diagnosis.

MED1 is a fully implemented, medical expert system providing assistance in the diagnosis of patients complaining of chest pain. Its reasoning strategy combines efficient mechanisms for hypothesis generation and hypothesis evaluation in a model simulalting the basic features of the hypothesize-and-test approach found to be applied by diagnosing physicians. The knowledge acquisition facility of the program is comfortable enough to allow the expert physician to alter the knowledge base without understanding the basic code (LISP) of the program.

Computers↗