Purification of Clostridium botulinum type A toxin.
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Biomedical subjects
Publications and source records attributed to B R Dasgupta.
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The toxic and hemagglutinating components of the crystalline toxin of Clostridium botulinum were analyzed for their amino acid content and were proven to be completely different proteins.
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Gerwing et al. described the isolation and purification from culture filtrates of the toxin of Clostridium botulinum type B and characterized it as a homogeneous protein of less than 10,000 molecular weight. Analysis by various methods of samples of this toxin obtained from Gerwing et al., and preparations produced by their methods in our laboratories, furnished convincing evidence that neither her preparation nor ours was homogeneous. The molecular weight of the toxic component isolated from either of the preparations was 100,000 or greater and resembled, in a number of respects, the alpha component isolated by us from the crystalline toxin of C. botulinum type A.
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Explore the source record for details and available documents.