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Biomedical subjects

B R Masters

Publications and source records attributed to B R Masters.

42 records · Page 3Linked to original sources
PubMedDate not supplied

Transformation of a set of slices rotated on a common axis to a set of Z-slices: application to three-dimensional visualization of the in vivo human lens.

A technique that transforms a set of images acquired as a rotating frame about an axis (Z axis) into a set of images along the Z axis in presented. This technique is applied to the three-dimensional visualization of the in vivo human lens. A Scheimpfling slit camera acquired 60 optical images through the in vivo human lens. Between each image acquisition the plane containing the slit beam of light was sequentially rotated. This set of 60 images was transformed into a new stack of images on the Z axis. The transformed stack of Z images was visualized with volume rendering software.

Aged↗
PubMedDate not supplied

Three-dimensional confocal microscopy of the human cornea in vivo.

This paper reviews the literature from 1990 to 2000 and evaluates the seminal investigations performed with the confocal microscope on the in vivo human cornea. Our pedagogical technique is to illustrate both the advantages and the problems associated with occular confocal microscopy by way of annotated examples. Confocal microscopy offers improved resolution and has resulted in new discoveries of corneal pathology at the cellular level. The ability to provide high-resolution, real-time images of the full thickness of the cornea gives the clinician and the researcher an important new tool for the investigation of the cornea.

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PubMedDate not supplied

In vivo flavoprotein redox measurements of rabbit corneal normoxic-anoxic transitions.

Reversible normoxic-anoxic transitions from in vivo rabbit corneas were measured and displayed as mitochondrial flavoprotein fluorescence histograms. The flavoproteins were excited with a Helium-Cadmium laser at 441.6 nm, and the fluorescence was detected in the region of 550 nm. The laser excitation light was incident at the apex of the cornea perpendicular to the optic axis, and the emission was detected along the optic axis. Artifacts due to the motion of the globe and fluorescence from the iris and the lens were not significant. These studies demonstrate the feasibility of non-invasive in vivo monitoring of corneal anoxia-normoxia, and suggest its future applications to studies with contact lens and corneal wound healing.

Animals↗
PubMedDate not supplied

Rabbit cornea stromal hydration measured with proton NMR spectroscopy.

The suitability of water proton spin-lattice relaxation time T1 as a measure of corneal dysfunction is investigated in this paper. Proton nuclear magnetic resonance measurements were performed on excised rabbit corneas using the saturation recovery method to determine the proton spin-lattice relaxation time T1. Both freshly excised and progressively swollen rabbit corneas were studied. The experimental results are consistent with a two compartment model of "bound" water and "free" water. This model results in a linear correlation between 1 / T1 (measured) and 1/corneal hydration. This correlation suggests that water proton T1 values can be used as an index of corneal dysfunction.

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